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Nitrite and Nitrate Levels in Follicular Fluid From Human Oocyte Donors Are Related to Ovarian Response and Embryo Quality

Nitric oxide, a key regulatory molecule in the follicular fluid, has been suggested as a possible biomarker to predict ovarian response in stimulated cycles and the potential of the retrieved oocytes for developing high-quality embryos. Nevertheless, a consensus on whether or not nitric oxide can he...

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Autores principales: Staicu, Florentin-Daniel, Canha-Gouveia, Analuce, Soriano-Úbeda, Cristina, Martínez-Soto, Juan Carlos, Adoamnei, Evdochia, Chavarro, Jorge E., Matás, Carmen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079729/
https://www.ncbi.nlm.nih.gov/pubmed/33937241
http://dx.doi.org/10.3389/fcell.2021.647002
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author Staicu, Florentin-Daniel
Canha-Gouveia, Analuce
Soriano-Úbeda, Cristina
Martínez-Soto, Juan Carlos
Adoamnei, Evdochia
Chavarro, Jorge E.
Matás, Carmen
author_facet Staicu, Florentin-Daniel
Canha-Gouveia, Analuce
Soriano-Úbeda, Cristina
Martínez-Soto, Juan Carlos
Adoamnei, Evdochia
Chavarro, Jorge E.
Matás, Carmen
author_sort Staicu, Florentin-Daniel
collection PubMed
description Nitric oxide, a key regulatory molecule in the follicular fluid, has been suggested as a possible biomarker to predict ovarian response in stimulated cycles and the potential of the retrieved oocytes for developing high-quality embryos. Nevertheless, a consensus on whether or not nitric oxide can help in this context has not been reached. We simultaneously measured the oxidation products of nitric oxide, nitrite, and nitrate, via high-performance liquid chromatography (HPLC)-UV in follicular fluid samples from 72 oocyte donors. We found no associations of follicular fluid nitrite, nitrate, total nitric oxide, or nitrate/nitrite ratio with total or metaphase II (MII) oocyte yield. However, nitrite and nitrate levels were related to the yield of MII oocytes when this outcome was expressed as a proportion of all oocytes retrieved. The adjusted MII proportion in the lowest and highest nitrite levels were 68% (58–77%) and 79% (70–85%), respectively (p, linear trend = 0.02), whereas the adjusted MII proportion in extreme tertiles of nitrate levels were 79% (70–85%) and 68% (57–77%) (p, linear trend = 0.03). In addition, nitrate levels showed a suggestive inverse correlation with embryos with maximum or high potential of implantation (p = 0.07). These results suggest that the follicular fluid concentrations of nitrite and nitrate may be a useful tool in predicting how healthy oocyte donors respond to superovulation and the implantation potential of the embryos produced from their oocytes.
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spelling pubmed-80797292021-04-29 Nitrite and Nitrate Levels in Follicular Fluid From Human Oocyte Donors Are Related to Ovarian Response and Embryo Quality Staicu, Florentin-Daniel Canha-Gouveia, Analuce Soriano-Úbeda, Cristina Martínez-Soto, Juan Carlos Adoamnei, Evdochia Chavarro, Jorge E. Matás, Carmen Front Cell Dev Biol Cell and Developmental Biology Nitric oxide, a key regulatory molecule in the follicular fluid, has been suggested as a possible biomarker to predict ovarian response in stimulated cycles and the potential of the retrieved oocytes for developing high-quality embryos. Nevertheless, a consensus on whether or not nitric oxide can help in this context has not been reached. We simultaneously measured the oxidation products of nitric oxide, nitrite, and nitrate, via high-performance liquid chromatography (HPLC)-UV in follicular fluid samples from 72 oocyte donors. We found no associations of follicular fluid nitrite, nitrate, total nitric oxide, or nitrate/nitrite ratio with total or metaphase II (MII) oocyte yield. However, nitrite and nitrate levels were related to the yield of MII oocytes when this outcome was expressed as a proportion of all oocytes retrieved. The adjusted MII proportion in the lowest and highest nitrite levels were 68% (58–77%) and 79% (70–85%), respectively (p, linear trend = 0.02), whereas the adjusted MII proportion in extreme tertiles of nitrate levels were 79% (70–85%) and 68% (57–77%) (p, linear trend = 0.03). In addition, nitrate levels showed a suggestive inverse correlation with embryos with maximum or high potential of implantation (p = 0.07). These results suggest that the follicular fluid concentrations of nitrite and nitrate may be a useful tool in predicting how healthy oocyte donors respond to superovulation and the implantation potential of the embryos produced from their oocytes. Frontiers Media S.A. 2021-04-14 /pmc/articles/PMC8079729/ /pubmed/33937241 http://dx.doi.org/10.3389/fcell.2021.647002 Text en Copyright © 2021 Staicu, Canha-Gouveia, Soriano-Úbeda, Martínez-Soto, Adoamnei, Chavarro and Matás. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Staicu, Florentin-Daniel
Canha-Gouveia, Analuce
Soriano-Úbeda, Cristina
Martínez-Soto, Juan Carlos
Adoamnei, Evdochia
Chavarro, Jorge E.
Matás, Carmen
Nitrite and Nitrate Levels in Follicular Fluid From Human Oocyte Donors Are Related to Ovarian Response and Embryo Quality
title Nitrite and Nitrate Levels in Follicular Fluid From Human Oocyte Donors Are Related to Ovarian Response and Embryo Quality
title_full Nitrite and Nitrate Levels in Follicular Fluid From Human Oocyte Donors Are Related to Ovarian Response and Embryo Quality
title_fullStr Nitrite and Nitrate Levels in Follicular Fluid From Human Oocyte Donors Are Related to Ovarian Response and Embryo Quality
title_full_unstemmed Nitrite and Nitrate Levels in Follicular Fluid From Human Oocyte Donors Are Related to Ovarian Response and Embryo Quality
title_short Nitrite and Nitrate Levels in Follicular Fluid From Human Oocyte Donors Are Related to Ovarian Response and Embryo Quality
title_sort nitrite and nitrate levels in follicular fluid from human oocyte donors are related to ovarian response and embryo quality
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079729/
https://www.ncbi.nlm.nih.gov/pubmed/33937241
http://dx.doi.org/10.3389/fcell.2021.647002
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