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Detection of EGFR Mutations From Plasma of NSCLC Patients Using an Automatic Cartridge-Based PCR System

The introduction of liquid biopsies for the detection of EGFR mutations in non-small cell lung cancer patients (NSCLC) has revolutionized the clinical care. However, liquid biopsies are technically challenging and require specifically trained personnel. To facilitate the implementation of liquid bio...

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Autores principales: Heeke, Simon, Hofman, Véronique, Benzaquen, Jonathan, Otto, Josiane, Tanga, Virginie, Zahaf, Katia, Allegra, Maryline, Long-Mira, Elodie, Lassalle, Sandra, Marquette, Charles-Hugo, Ilie, Marius, Hofman, Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079969/
https://www.ncbi.nlm.nih.gov/pubmed/33935776
http://dx.doi.org/10.3389/fphar.2021.657743
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author Heeke, Simon
Hofman, Véronique
Benzaquen, Jonathan
Otto, Josiane
Tanga, Virginie
Zahaf, Katia
Allegra, Maryline
Long-Mira, Elodie
Lassalle, Sandra
Marquette, Charles-Hugo
Ilie, Marius
Hofman, Paul
author_facet Heeke, Simon
Hofman, Véronique
Benzaquen, Jonathan
Otto, Josiane
Tanga, Virginie
Zahaf, Katia
Allegra, Maryline
Long-Mira, Elodie
Lassalle, Sandra
Marquette, Charles-Hugo
Ilie, Marius
Hofman, Paul
author_sort Heeke, Simon
collection PubMed
description The introduction of liquid biopsies for the detection of EGFR mutations in non-small cell lung cancer patients (NSCLC) has revolutionized the clinical care. However, liquid biopsies are technically challenging and require specifically trained personnel. To facilitate the implementation of liquid biopsies for the detection of EGFR mutations from plasma, we have assessed a fully automated cartridge-based qPCR test that allows the automatic detection of EGFR mutations directly from plasma. We have analyzed 54 NSCLC patients and compared the results of the cartridge-base device to an FDA-approved assay. Detection of EGFR mutations was comparable but slightly lower in the cartridge-based device for L858R mutations (14/15 detected, 93%) and exon 19 deletions (18/20 detected, 90%). Unfortunately, 8/54 (15%) tests failed but increasing the proteinase K volume helped to recover 3/4 (75%) unsuccessful samples. In summary, the fully automated cartridge-based device allowed the detection of EGFR mutations directly from plasma in NSCLC patients with promising accuracy. However, protocol adjustments are necessary to reduce a high test failure rate.
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spelling pubmed-80799692021-04-29 Detection of EGFR Mutations From Plasma of NSCLC Patients Using an Automatic Cartridge-Based PCR System Heeke, Simon Hofman, Véronique Benzaquen, Jonathan Otto, Josiane Tanga, Virginie Zahaf, Katia Allegra, Maryline Long-Mira, Elodie Lassalle, Sandra Marquette, Charles-Hugo Ilie, Marius Hofman, Paul Front Pharmacol Pharmacology The introduction of liquid biopsies for the detection of EGFR mutations in non-small cell lung cancer patients (NSCLC) has revolutionized the clinical care. However, liquid biopsies are technically challenging and require specifically trained personnel. To facilitate the implementation of liquid biopsies for the detection of EGFR mutations from plasma, we have assessed a fully automated cartridge-based qPCR test that allows the automatic detection of EGFR mutations directly from plasma. We have analyzed 54 NSCLC patients and compared the results of the cartridge-base device to an FDA-approved assay. Detection of EGFR mutations was comparable but slightly lower in the cartridge-based device for L858R mutations (14/15 detected, 93%) and exon 19 deletions (18/20 detected, 90%). Unfortunately, 8/54 (15%) tests failed but increasing the proteinase K volume helped to recover 3/4 (75%) unsuccessful samples. In summary, the fully automated cartridge-based device allowed the detection of EGFR mutations directly from plasma in NSCLC patients with promising accuracy. However, protocol adjustments are necessary to reduce a high test failure rate. Frontiers Media S.A. 2021-04-14 /pmc/articles/PMC8079969/ /pubmed/33935776 http://dx.doi.org/10.3389/fphar.2021.657743 Text en Copyright © 2021 Heeke, Hofman, Benzaquen, Otto, Tanga, Zahaf, Allegra, Long-Mira, Lassalle, Marquette, Ilie and Hofman. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Heeke, Simon
Hofman, Véronique
Benzaquen, Jonathan
Otto, Josiane
Tanga, Virginie
Zahaf, Katia
Allegra, Maryline
Long-Mira, Elodie
Lassalle, Sandra
Marquette, Charles-Hugo
Ilie, Marius
Hofman, Paul
Detection of EGFR Mutations From Plasma of NSCLC Patients Using an Automatic Cartridge-Based PCR System
title Detection of EGFR Mutations From Plasma of NSCLC Patients Using an Automatic Cartridge-Based PCR System
title_full Detection of EGFR Mutations From Plasma of NSCLC Patients Using an Automatic Cartridge-Based PCR System
title_fullStr Detection of EGFR Mutations From Plasma of NSCLC Patients Using an Automatic Cartridge-Based PCR System
title_full_unstemmed Detection of EGFR Mutations From Plasma of NSCLC Patients Using an Automatic Cartridge-Based PCR System
title_short Detection of EGFR Mutations From Plasma of NSCLC Patients Using an Automatic Cartridge-Based PCR System
title_sort detection of egfr mutations from plasma of nsclc patients using an automatic cartridge-based pcr system
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079969/
https://www.ncbi.nlm.nih.gov/pubmed/33935776
http://dx.doi.org/10.3389/fphar.2021.657743
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