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Detect and destroy: CRISPR-based technologies for the response against viruses

Despite numerous viral outbreaks in the last decade, including a devastating global pandemic, diagnostic and therapeutic technologies remain severely lacking. CRISPR-Cas systems have the potential to address these critical needs in the response against infectious disease. Initially discovered as the...

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Detalles Bibliográficos
Autores principales: Freije, Catherine A., Sabeti, Pardis C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Authors. Published by Elsevier Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8080417/
https://www.ncbi.nlm.nih.gov/pubmed/33915112
http://dx.doi.org/10.1016/j.chom.2021.04.003
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author Freije, Catherine A.
Sabeti, Pardis C.
author_facet Freije, Catherine A.
Sabeti, Pardis C.
author_sort Freije, Catherine A.
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description Despite numerous viral outbreaks in the last decade, including a devastating global pandemic, diagnostic and therapeutic technologies remain severely lacking. CRISPR-Cas systems have the potential to address these critical needs in the response against infectious disease. Initially discovered as the bacterial adaptive immune system, these systems provide a unique opportunity to create programmable, sequence-specific technologies for detection of viral nucleic acids and inhibition of viral replication. This review summarizes how CRISPR-Cas systems—in particular the recently discovered DNA-targeting Cas12 and RNA-targeting Cas13, both possessing a unique trans-cleavage activity—are being harnessed for viral diagnostics and therapies. We further highlight the numerous technologies whose development has accelerated in response to the COVID-19 pandemic.
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spelling pubmed-80804172021-04-28 Detect and destroy: CRISPR-based technologies for the response against viruses Freije, Catherine A. Sabeti, Pardis C. Cell Host Microbe Review Despite numerous viral outbreaks in the last decade, including a devastating global pandemic, diagnostic and therapeutic technologies remain severely lacking. CRISPR-Cas systems have the potential to address these critical needs in the response against infectious disease. Initially discovered as the bacterial adaptive immune system, these systems provide a unique opportunity to create programmable, sequence-specific technologies for detection of viral nucleic acids and inhibition of viral replication. This review summarizes how CRISPR-Cas systems—in particular the recently discovered DNA-targeting Cas12 and RNA-targeting Cas13, both possessing a unique trans-cleavage activity—are being harnessed for viral diagnostics and therapies. We further highlight the numerous technologies whose development has accelerated in response to the COVID-19 pandemic. The Authors. Published by Elsevier Inc. 2021-05-12 2021-04-28 /pmc/articles/PMC8080417/ /pubmed/33915112 http://dx.doi.org/10.1016/j.chom.2021.04.003 Text en © 2021 The Authors Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Review
Freije, Catherine A.
Sabeti, Pardis C.
Detect and destroy: CRISPR-based technologies for the response against viruses
title Detect and destroy: CRISPR-based technologies for the response against viruses
title_full Detect and destroy: CRISPR-based technologies for the response against viruses
title_fullStr Detect and destroy: CRISPR-based technologies for the response against viruses
title_full_unstemmed Detect and destroy: CRISPR-based technologies for the response against viruses
title_short Detect and destroy: CRISPR-based technologies for the response against viruses
title_sort detect and destroy: crispr-based technologies for the response against viruses
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8080417/
https://www.ncbi.nlm.nih.gov/pubmed/33915112
http://dx.doi.org/10.1016/j.chom.2021.04.003
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