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Identification of antigenic linear peptides in the soil-transmitted helminth and Schistosoma mansoni proteome
The scientific community identified non stool-based biomarkers as the way forward to support soil-transmitted helminth (STH; Ascaris lumbricoides, Trichuris trichiura and the hookworms Ancylostoma duodenale and Necator americanus) and schistosome (S. mansoni and S. haematobium) deworming programs. T...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081252/ https://www.ncbi.nlm.nih.gov/pubmed/33909616 http://dx.doi.org/10.1371/journal.pntd.0009369 |
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author | Vlaminck, Johnny Lagatie, Ole Dana, Daniel Mekonnen, Zeleke Geldhof, Peter Levecke, Bruno Stuyver, Lieven J. |
author_facet | Vlaminck, Johnny Lagatie, Ole Dana, Daniel Mekonnen, Zeleke Geldhof, Peter Levecke, Bruno Stuyver, Lieven J. |
author_sort | Vlaminck, Johnny |
collection | PubMed |
description | The scientific community identified non stool-based biomarkers as the way forward to support soil-transmitted helminth (STH; Ascaris lumbricoides, Trichuris trichiura and the hookworms Ancylostoma duodenale and Necator americanus) and schistosome (S. mansoni and S. haematobium) deworming programs. This support is needed in making the decision of whether or not to stop preventive chemotherapy intervention efforts and to ultimately transition towards a post-intervention surveillance phase. We applied a two-step micro-array approach to identify antigenic linear epitopes in the STH and S. mansoni proteomes. In a first experiment, we identified antigenic peptides by applying sera from 24 STH and/or S. mansoni infected Ethiopian children on a high-density peptide microarray containing 3.3 million peptides derived from the complete STH and S. mansoni proteomes. A second array experiment with 170,185 peptides that were recognized in the first array was designed to identify non-specific antibody reactivity by applying sera from 24 healthy individuals from Belgium (a non-endemic country). From this array testing cascade, several peptides were identified for STH but none of them appeared to be unique for one species. We therefore concluded that for STH, none of the peptides revealed to be sufficiently sensitive or species specific. For S. mansoni, some promising peptides were identified prompting future investigation. Based on these results, it is unlikely that linear epitopes would be highly useful in detecting species-specific antibody responses to STH in endemic communities. For S. mansoni, one particular peptide of the micro-exon gene 12 (MEG-12) protein deserves further research. In addition, this study emphasizes the need of well-characterized biobanks for biomarker discovery, particularly when the integration of multiple disease programs is envisioned. |
format | Online Article Text |
id | pubmed-8081252 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-80812522021-05-06 Identification of antigenic linear peptides in the soil-transmitted helminth and Schistosoma mansoni proteome Vlaminck, Johnny Lagatie, Ole Dana, Daniel Mekonnen, Zeleke Geldhof, Peter Levecke, Bruno Stuyver, Lieven J. PLoS Negl Trop Dis Research Article The scientific community identified non stool-based biomarkers as the way forward to support soil-transmitted helminth (STH; Ascaris lumbricoides, Trichuris trichiura and the hookworms Ancylostoma duodenale and Necator americanus) and schistosome (S. mansoni and S. haematobium) deworming programs. This support is needed in making the decision of whether or not to stop preventive chemotherapy intervention efforts and to ultimately transition towards a post-intervention surveillance phase. We applied a two-step micro-array approach to identify antigenic linear epitopes in the STH and S. mansoni proteomes. In a first experiment, we identified antigenic peptides by applying sera from 24 STH and/or S. mansoni infected Ethiopian children on a high-density peptide microarray containing 3.3 million peptides derived from the complete STH and S. mansoni proteomes. A second array experiment with 170,185 peptides that were recognized in the first array was designed to identify non-specific antibody reactivity by applying sera from 24 healthy individuals from Belgium (a non-endemic country). From this array testing cascade, several peptides were identified for STH but none of them appeared to be unique for one species. We therefore concluded that for STH, none of the peptides revealed to be sufficiently sensitive or species specific. For S. mansoni, some promising peptides were identified prompting future investigation. Based on these results, it is unlikely that linear epitopes would be highly useful in detecting species-specific antibody responses to STH in endemic communities. For S. mansoni, one particular peptide of the micro-exon gene 12 (MEG-12) protein deserves further research. In addition, this study emphasizes the need of well-characterized biobanks for biomarker discovery, particularly when the integration of multiple disease programs is envisioned. Public Library of Science 2021-04-28 /pmc/articles/PMC8081252/ /pubmed/33909616 http://dx.doi.org/10.1371/journal.pntd.0009369 Text en © 2021 Vlaminck et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Vlaminck, Johnny Lagatie, Ole Dana, Daniel Mekonnen, Zeleke Geldhof, Peter Levecke, Bruno Stuyver, Lieven J. Identification of antigenic linear peptides in the soil-transmitted helminth and Schistosoma mansoni proteome |
title | Identification of antigenic linear peptides in the soil-transmitted helminth and Schistosoma mansoni proteome |
title_full | Identification of antigenic linear peptides in the soil-transmitted helminth and Schistosoma mansoni proteome |
title_fullStr | Identification of antigenic linear peptides in the soil-transmitted helminth and Schistosoma mansoni proteome |
title_full_unstemmed | Identification of antigenic linear peptides in the soil-transmitted helminth and Schistosoma mansoni proteome |
title_short | Identification of antigenic linear peptides in the soil-transmitted helminth and Schistosoma mansoni proteome |
title_sort | identification of antigenic linear peptides in the soil-transmitted helminth and schistosoma mansoni proteome |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081252/ https://www.ncbi.nlm.nih.gov/pubmed/33909616 http://dx.doi.org/10.1371/journal.pntd.0009369 |
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