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Identification of antigenic linear peptides in the soil-transmitted helminth and Schistosoma mansoni proteome

The scientific community identified non stool-based biomarkers as the way forward to support soil-transmitted helminth (STH; Ascaris lumbricoides, Trichuris trichiura and the hookworms Ancylostoma duodenale and Necator americanus) and schistosome (S. mansoni and S. haematobium) deworming programs. T...

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Autores principales: Vlaminck, Johnny, Lagatie, Ole, Dana, Daniel, Mekonnen, Zeleke, Geldhof, Peter, Levecke, Bruno, Stuyver, Lieven J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081252/
https://www.ncbi.nlm.nih.gov/pubmed/33909616
http://dx.doi.org/10.1371/journal.pntd.0009369
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author Vlaminck, Johnny
Lagatie, Ole
Dana, Daniel
Mekonnen, Zeleke
Geldhof, Peter
Levecke, Bruno
Stuyver, Lieven J.
author_facet Vlaminck, Johnny
Lagatie, Ole
Dana, Daniel
Mekonnen, Zeleke
Geldhof, Peter
Levecke, Bruno
Stuyver, Lieven J.
author_sort Vlaminck, Johnny
collection PubMed
description The scientific community identified non stool-based biomarkers as the way forward to support soil-transmitted helminth (STH; Ascaris lumbricoides, Trichuris trichiura and the hookworms Ancylostoma duodenale and Necator americanus) and schistosome (S. mansoni and S. haematobium) deworming programs. This support is needed in making the decision of whether or not to stop preventive chemotherapy intervention efforts and to ultimately transition towards a post-intervention surveillance phase. We applied a two-step micro-array approach to identify antigenic linear epitopes in the STH and S. mansoni proteomes. In a first experiment, we identified antigenic peptides by applying sera from 24 STH and/or S. mansoni infected Ethiopian children on a high-density peptide microarray containing 3.3 million peptides derived from the complete STH and S. mansoni proteomes. A second array experiment with 170,185 peptides that were recognized in the first array was designed to identify non-specific antibody reactivity by applying sera from 24 healthy individuals from Belgium (a non-endemic country). From this array testing cascade, several peptides were identified for STH but none of them appeared to be unique for one species. We therefore concluded that for STH, none of the peptides revealed to be sufficiently sensitive or species specific. For S. mansoni, some promising peptides were identified prompting future investigation. Based on these results, it is unlikely that linear epitopes would be highly useful in detecting species-specific antibody responses to STH in endemic communities. For S. mansoni, one particular peptide of the micro-exon gene 12 (MEG-12) protein deserves further research. In addition, this study emphasizes the need of well-characterized biobanks for biomarker discovery, particularly when the integration of multiple disease programs is envisioned.
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spelling pubmed-80812522021-05-06 Identification of antigenic linear peptides in the soil-transmitted helminth and Schistosoma mansoni proteome Vlaminck, Johnny Lagatie, Ole Dana, Daniel Mekonnen, Zeleke Geldhof, Peter Levecke, Bruno Stuyver, Lieven J. PLoS Negl Trop Dis Research Article The scientific community identified non stool-based biomarkers as the way forward to support soil-transmitted helminth (STH; Ascaris lumbricoides, Trichuris trichiura and the hookworms Ancylostoma duodenale and Necator americanus) and schistosome (S. mansoni and S. haematobium) deworming programs. This support is needed in making the decision of whether or not to stop preventive chemotherapy intervention efforts and to ultimately transition towards a post-intervention surveillance phase. We applied a two-step micro-array approach to identify antigenic linear epitopes in the STH and S. mansoni proteomes. In a first experiment, we identified antigenic peptides by applying sera from 24 STH and/or S. mansoni infected Ethiopian children on a high-density peptide microarray containing 3.3 million peptides derived from the complete STH and S. mansoni proteomes. A second array experiment with 170,185 peptides that were recognized in the first array was designed to identify non-specific antibody reactivity by applying sera from 24 healthy individuals from Belgium (a non-endemic country). From this array testing cascade, several peptides were identified for STH but none of them appeared to be unique for one species. We therefore concluded that for STH, none of the peptides revealed to be sufficiently sensitive or species specific. For S. mansoni, some promising peptides were identified prompting future investigation. Based on these results, it is unlikely that linear epitopes would be highly useful in detecting species-specific antibody responses to STH in endemic communities. For S. mansoni, one particular peptide of the micro-exon gene 12 (MEG-12) protein deserves further research. In addition, this study emphasizes the need of well-characterized biobanks for biomarker discovery, particularly when the integration of multiple disease programs is envisioned. Public Library of Science 2021-04-28 /pmc/articles/PMC8081252/ /pubmed/33909616 http://dx.doi.org/10.1371/journal.pntd.0009369 Text en © 2021 Vlaminck et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Vlaminck, Johnny
Lagatie, Ole
Dana, Daniel
Mekonnen, Zeleke
Geldhof, Peter
Levecke, Bruno
Stuyver, Lieven J.
Identification of antigenic linear peptides in the soil-transmitted helminth and Schistosoma mansoni proteome
title Identification of antigenic linear peptides in the soil-transmitted helminth and Schistosoma mansoni proteome
title_full Identification of antigenic linear peptides in the soil-transmitted helminth and Schistosoma mansoni proteome
title_fullStr Identification of antigenic linear peptides in the soil-transmitted helminth and Schistosoma mansoni proteome
title_full_unstemmed Identification of antigenic linear peptides in the soil-transmitted helminth and Schistosoma mansoni proteome
title_short Identification of antigenic linear peptides in the soil-transmitted helminth and Schistosoma mansoni proteome
title_sort identification of antigenic linear peptides in the soil-transmitted helminth and schistosoma mansoni proteome
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081252/
https://www.ncbi.nlm.nih.gov/pubmed/33909616
http://dx.doi.org/10.1371/journal.pntd.0009369
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