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Micro-biopsy for detection of gene expression changes in ischemic swine myocardium: A pilot study

Micro-endomyocardial biopsy (micro-EMB) is a novel catheter-based biopsy technique, aiming to increase flexibility and safety compared to conventional EMB. The technique was developed and evaluated in healthy swine. Therefore, the ability to detect disease related tissue changes could not be evaluat...

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Autores principales: Chireh, Arvin, Grankvist, Rikard, Sandell, Mikael, Mukarram, Abdul Kadir, Arnberg, Fabian, Lundberg, Johan, Daub, Carsten O., Holmin, Staffan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081259/
https://www.ncbi.nlm.nih.gov/pubmed/33909677
http://dx.doi.org/10.1371/journal.pone.0250582
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author Chireh, Arvin
Grankvist, Rikard
Sandell, Mikael
Mukarram, Abdul Kadir
Arnberg, Fabian
Lundberg, Johan
Daub, Carsten O.
Holmin, Staffan
author_facet Chireh, Arvin
Grankvist, Rikard
Sandell, Mikael
Mukarram, Abdul Kadir
Arnberg, Fabian
Lundberg, Johan
Daub, Carsten O.
Holmin, Staffan
author_sort Chireh, Arvin
collection PubMed
description Micro-endomyocardial biopsy (micro-EMB) is a novel catheter-based biopsy technique, aiming to increase flexibility and safety compared to conventional EMB. The technique was developed and evaluated in healthy swine. Therefore, the ability to detect disease related tissue changes could not be evaluated. The aim of the present pilot study was to investigate the ability to detect disease related gene expression changes using micro-EMB. Myocardial infarction was induced in three swine by coronary artery balloon occlusion. Micro-EMB samples (n = 164) were collected before, during, and after occlusion. RNA-sequencing was performed on 85 samples, and 53 of these were selected for bioinformatic analysis. A large number of responding genes was detected from the infarcted area (n = 1911). The early responding genes (n = 1268) were mostly related to apoptosis and inflammation. There were fewer responding genes two days after infarction (n = 6), which were related to extra-cellular matrix changes, and none after 14 days. In contrast to the infarcted area, samples harvested from a non-infarcted myocardial region showed considerably fewer regulated genes (n = 33). Deconvolution analysis, to estimate the proportion of different cell types, revealed a higher proportion of fibroblasts and a reduced proportion of cardiomyocytes two days after occlusion compared to baseline (p < 0.02 and p < 0.01, respectively. S5 File). In conclusion, this pilot study demonstrates the capabilities of micro-EMB to detect local gene expression responses at an early stage after ischemia, but not at later timepoints.
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spelling pubmed-80812592021-05-06 Micro-biopsy for detection of gene expression changes in ischemic swine myocardium: A pilot study Chireh, Arvin Grankvist, Rikard Sandell, Mikael Mukarram, Abdul Kadir Arnberg, Fabian Lundberg, Johan Daub, Carsten O. Holmin, Staffan PLoS One Research Article Micro-endomyocardial biopsy (micro-EMB) is a novel catheter-based biopsy technique, aiming to increase flexibility and safety compared to conventional EMB. The technique was developed and evaluated in healthy swine. Therefore, the ability to detect disease related tissue changes could not be evaluated. The aim of the present pilot study was to investigate the ability to detect disease related gene expression changes using micro-EMB. Myocardial infarction was induced in three swine by coronary artery balloon occlusion. Micro-EMB samples (n = 164) were collected before, during, and after occlusion. RNA-sequencing was performed on 85 samples, and 53 of these were selected for bioinformatic analysis. A large number of responding genes was detected from the infarcted area (n = 1911). The early responding genes (n = 1268) were mostly related to apoptosis and inflammation. There were fewer responding genes two days after infarction (n = 6), which were related to extra-cellular matrix changes, and none after 14 days. In contrast to the infarcted area, samples harvested from a non-infarcted myocardial region showed considerably fewer regulated genes (n = 33). Deconvolution analysis, to estimate the proportion of different cell types, revealed a higher proportion of fibroblasts and a reduced proportion of cardiomyocytes two days after occlusion compared to baseline (p < 0.02 and p < 0.01, respectively. S5 File). In conclusion, this pilot study demonstrates the capabilities of micro-EMB to detect local gene expression responses at an early stage after ischemia, but not at later timepoints. Public Library of Science 2021-04-28 /pmc/articles/PMC8081259/ /pubmed/33909677 http://dx.doi.org/10.1371/journal.pone.0250582 Text en © 2021 Chireh et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Chireh, Arvin
Grankvist, Rikard
Sandell, Mikael
Mukarram, Abdul Kadir
Arnberg, Fabian
Lundberg, Johan
Daub, Carsten O.
Holmin, Staffan
Micro-biopsy for detection of gene expression changes in ischemic swine myocardium: A pilot study
title Micro-biopsy for detection of gene expression changes in ischemic swine myocardium: A pilot study
title_full Micro-biopsy for detection of gene expression changes in ischemic swine myocardium: A pilot study
title_fullStr Micro-biopsy for detection of gene expression changes in ischemic swine myocardium: A pilot study
title_full_unstemmed Micro-biopsy for detection of gene expression changes in ischemic swine myocardium: A pilot study
title_short Micro-biopsy for detection of gene expression changes in ischemic swine myocardium: A pilot study
title_sort micro-biopsy for detection of gene expression changes in ischemic swine myocardium: a pilot study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081259/
https://www.ncbi.nlm.nih.gov/pubmed/33909677
http://dx.doi.org/10.1371/journal.pone.0250582
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