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Timed Electrodeposition of PEDOT:Nafion onto Carbon Fiber-Microelectrodes Enhances Dopamine Detection in Zebrafish Retina
Carbon fiber-microelectrodes (CFMEs) are one of the standards for the detection of neurotransmitters such as dopamine (DA). In this study, we demonstrate that CFMEs electrodeposited with poly (3,4-ethylenedioxythiophene) (PEDOT) in the presence of Nafion exhibit enhanced sensitivity for DA detection...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081298/ https://www.ncbi.nlm.nih.gov/pubmed/33927449 http://dx.doi.org/10.1149/1945-7111/aba33d |
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author | Cho, Whirang Liu, Favian Hendrix, Aaron McCray, Brazil Asrat, Thomas Connaughton, Victoria Zestos, Alexander G. |
author_facet | Cho, Whirang Liu, Favian Hendrix, Aaron McCray, Brazil Asrat, Thomas Connaughton, Victoria Zestos, Alexander G. |
author_sort | Cho, Whirang |
collection | PubMed |
description | Carbon fiber-microelectrodes (CFMEs) are one of the standards for the detection of neurotransmitters such as dopamine (DA). In this study, we demonstrate that CFMEs electrodeposited with poly (3,4-ethylenedioxythiophene) (PEDOT) in the presence of Nafion exhibit enhanced sensitivity for DA detection. Scanning electron microscopy (SEM) revealed the smooth outer surface morphologies of polymer coatings, which filled in the ridges and grooves of the bare unmodified carbon electrode and energy-dispersive X-ray spectroscopy (EDX) confirmed PEDOT:Nafion incorporation. PEDOT:Nafion coated CMFEs exhibited a statistically enhanced two-fold increase in DA sensitivity compared to unmodified microelectrodes, with stability and integrity of the coated microelectrodes maintained for at least 4 h. A scan rate test revealed a linear relationship with peak DA oxidative current (5 μM), indicating adsorption control of DA to the surface of the PEDOT:Nafion electrode. As proof of principle, PEDOT:Nafion coated electrodes were used to detect potassium chloride (KCl)-induced DA release in zebrafish (Danio rerio) retinal tissue ex vivo, thus illustrating their applicability as biosensors. |
format | Online Article Text |
id | pubmed-8081298 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
record_format | MEDLINE/PubMed |
spelling | pubmed-80812982021-04-28 Timed Electrodeposition of PEDOT:Nafion onto Carbon Fiber-Microelectrodes Enhances Dopamine Detection in Zebrafish Retina Cho, Whirang Liu, Favian Hendrix, Aaron McCray, Brazil Asrat, Thomas Connaughton, Victoria Zestos, Alexander G. J Electrochem Soc Article Carbon fiber-microelectrodes (CFMEs) are one of the standards for the detection of neurotransmitters such as dopamine (DA). In this study, we demonstrate that CFMEs electrodeposited with poly (3,4-ethylenedioxythiophene) (PEDOT) in the presence of Nafion exhibit enhanced sensitivity for DA detection. Scanning electron microscopy (SEM) revealed the smooth outer surface morphologies of polymer coatings, which filled in the ridges and grooves of the bare unmodified carbon electrode and energy-dispersive X-ray spectroscopy (EDX) confirmed PEDOT:Nafion incorporation. PEDOT:Nafion coated CMFEs exhibited a statistically enhanced two-fold increase in DA sensitivity compared to unmodified microelectrodes, with stability and integrity of the coated microelectrodes maintained for at least 4 h. A scan rate test revealed a linear relationship with peak DA oxidative current (5 μM), indicating adsorption control of DA to the surface of the PEDOT:Nafion electrode. As proof of principle, PEDOT:Nafion coated electrodes were used to detect potassium chloride (KCl)-induced DA release in zebrafish (Danio rerio) retinal tissue ex vivo, thus illustrating their applicability as biosensors. 2020-07-14 2020-08 /pmc/articles/PMC8081298/ /pubmed/33927449 http://dx.doi.org/10.1149/1945-7111/aba33d Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives 4.0 License (CC BY-NC-ND, http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is not changed in any way and is properly cited. For permission for commercial reuse, permissions@ioppublishing.org. |
spellingShingle | Article Cho, Whirang Liu, Favian Hendrix, Aaron McCray, Brazil Asrat, Thomas Connaughton, Victoria Zestos, Alexander G. Timed Electrodeposition of PEDOT:Nafion onto Carbon Fiber-Microelectrodes Enhances Dopamine Detection in Zebrafish Retina |
title | Timed Electrodeposition of PEDOT:Nafion onto Carbon Fiber-Microelectrodes Enhances Dopamine Detection in Zebrafish Retina |
title_full | Timed Electrodeposition of PEDOT:Nafion onto Carbon Fiber-Microelectrodes Enhances Dopamine Detection in Zebrafish Retina |
title_fullStr | Timed Electrodeposition of PEDOT:Nafion onto Carbon Fiber-Microelectrodes Enhances Dopamine Detection in Zebrafish Retina |
title_full_unstemmed | Timed Electrodeposition of PEDOT:Nafion onto Carbon Fiber-Microelectrodes Enhances Dopamine Detection in Zebrafish Retina |
title_short | Timed Electrodeposition of PEDOT:Nafion onto Carbon Fiber-Microelectrodes Enhances Dopamine Detection in Zebrafish Retina |
title_sort | timed electrodeposition of pedot:nafion onto carbon fiber-microelectrodes enhances dopamine detection in zebrafish retina |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081298/ https://www.ncbi.nlm.nih.gov/pubmed/33927449 http://dx.doi.org/10.1149/1945-7111/aba33d |
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