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Timed Electrodeposition of PEDOT:Nafion onto Carbon Fiber-Microelectrodes Enhances Dopamine Detection in Zebrafish Retina

Carbon fiber-microelectrodes (CFMEs) are one of the standards for the detection of neurotransmitters such as dopamine (DA). In this study, we demonstrate that CFMEs electrodeposited with poly (3,4-ethylenedioxythiophene) (PEDOT) in the presence of Nafion exhibit enhanced sensitivity for DA detection...

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Autores principales: Cho, Whirang, Liu, Favian, Hendrix, Aaron, McCray, Brazil, Asrat, Thomas, Connaughton, Victoria, Zestos, Alexander G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081298/
https://www.ncbi.nlm.nih.gov/pubmed/33927449
http://dx.doi.org/10.1149/1945-7111/aba33d
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author Cho, Whirang
Liu, Favian
Hendrix, Aaron
McCray, Brazil
Asrat, Thomas
Connaughton, Victoria
Zestos, Alexander G.
author_facet Cho, Whirang
Liu, Favian
Hendrix, Aaron
McCray, Brazil
Asrat, Thomas
Connaughton, Victoria
Zestos, Alexander G.
author_sort Cho, Whirang
collection PubMed
description Carbon fiber-microelectrodes (CFMEs) are one of the standards for the detection of neurotransmitters such as dopamine (DA). In this study, we demonstrate that CFMEs electrodeposited with poly (3,4-ethylenedioxythiophene) (PEDOT) in the presence of Nafion exhibit enhanced sensitivity for DA detection. Scanning electron microscopy (SEM) revealed the smooth outer surface morphologies of polymer coatings, which filled in the ridges and grooves of the bare unmodified carbon electrode and energy-dispersive X-ray spectroscopy (EDX) confirmed PEDOT:Nafion incorporation. PEDOT:Nafion coated CMFEs exhibited a statistically enhanced two-fold increase in DA sensitivity compared to unmodified microelectrodes, with stability and integrity of the coated microelectrodes maintained for at least 4 h. A scan rate test revealed a linear relationship with peak DA oxidative current (5 μM), indicating adsorption control of DA to the surface of the PEDOT:Nafion electrode. As proof of principle, PEDOT:Nafion coated electrodes were used to detect potassium chloride (KCl)-induced DA release in zebrafish (Danio rerio) retinal tissue ex vivo, thus illustrating their applicability as biosensors.
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spelling pubmed-80812982021-04-28 Timed Electrodeposition of PEDOT:Nafion onto Carbon Fiber-Microelectrodes Enhances Dopamine Detection in Zebrafish Retina Cho, Whirang Liu, Favian Hendrix, Aaron McCray, Brazil Asrat, Thomas Connaughton, Victoria Zestos, Alexander G. J Electrochem Soc Article Carbon fiber-microelectrodes (CFMEs) are one of the standards for the detection of neurotransmitters such as dopamine (DA). In this study, we demonstrate that CFMEs electrodeposited with poly (3,4-ethylenedioxythiophene) (PEDOT) in the presence of Nafion exhibit enhanced sensitivity for DA detection. Scanning electron microscopy (SEM) revealed the smooth outer surface morphologies of polymer coatings, which filled in the ridges and grooves of the bare unmodified carbon electrode and energy-dispersive X-ray spectroscopy (EDX) confirmed PEDOT:Nafion incorporation. PEDOT:Nafion coated CMFEs exhibited a statistically enhanced two-fold increase in DA sensitivity compared to unmodified microelectrodes, with stability and integrity of the coated microelectrodes maintained for at least 4 h. A scan rate test revealed a linear relationship with peak DA oxidative current (5 μM), indicating adsorption control of DA to the surface of the PEDOT:Nafion electrode. As proof of principle, PEDOT:Nafion coated electrodes were used to detect potassium chloride (KCl)-induced DA release in zebrafish (Danio rerio) retinal tissue ex vivo, thus illustrating their applicability as biosensors. 2020-07-14 2020-08 /pmc/articles/PMC8081298/ /pubmed/33927449 http://dx.doi.org/10.1149/1945-7111/aba33d Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives 4.0 License (CC BY-NC-ND, http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is not changed in any way and is properly cited. For permission for commercial reuse, permissions@ioppublishing.org.
spellingShingle Article
Cho, Whirang
Liu, Favian
Hendrix, Aaron
McCray, Brazil
Asrat, Thomas
Connaughton, Victoria
Zestos, Alexander G.
Timed Electrodeposition of PEDOT:Nafion onto Carbon Fiber-Microelectrodes Enhances Dopamine Detection in Zebrafish Retina
title Timed Electrodeposition of PEDOT:Nafion onto Carbon Fiber-Microelectrodes Enhances Dopamine Detection in Zebrafish Retina
title_full Timed Electrodeposition of PEDOT:Nafion onto Carbon Fiber-Microelectrodes Enhances Dopamine Detection in Zebrafish Retina
title_fullStr Timed Electrodeposition of PEDOT:Nafion onto Carbon Fiber-Microelectrodes Enhances Dopamine Detection in Zebrafish Retina
title_full_unstemmed Timed Electrodeposition of PEDOT:Nafion onto Carbon Fiber-Microelectrodes Enhances Dopamine Detection in Zebrafish Retina
title_short Timed Electrodeposition of PEDOT:Nafion onto Carbon Fiber-Microelectrodes Enhances Dopamine Detection in Zebrafish Retina
title_sort timed electrodeposition of pedot:nafion onto carbon fiber-microelectrodes enhances dopamine detection in zebrafish retina
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081298/
https://www.ncbi.nlm.nih.gov/pubmed/33927449
http://dx.doi.org/10.1149/1945-7111/aba33d
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