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Ultrafast pulse shaping modulates perceived visual brightness in living animals

Vision is usually assumed to be sensitive to the light intensity and spectrum but not to its spectral phase. However, experiments performed on retinal proteins in solution showed that the first step of vision consists in an ultrafast photoisomerization that can be coherently controlled by shaping th...

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Autores principales: Gaulier, Geoffrey, Dietschi, Quentin, Bhattacharyya, Swarnendu, Schmidt, Cédric, Montagnese, Matteo, Chauvet, Adrien, Hermelin, Sylvain, Chiodini, Florence, Bonacina, Luigi, Herrera, Pedro L., Rothlisberger, Ursula, Rodriguez, Ivan, Wolf, Jean-Pierre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081367/
https://www.ncbi.nlm.nih.gov/pubmed/33910906
http://dx.doi.org/10.1126/sciadv.abe1911
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author Gaulier, Geoffrey
Dietschi, Quentin
Bhattacharyya, Swarnendu
Schmidt, Cédric
Montagnese, Matteo
Chauvet, Adrien
Hermelin, Sylvain
Chiodini, Florence
Bonacina, Luigi
Herrera, Pedro L.
Rothlisberger, Ursula
Rodriguez, Ivan
Wolf, Jean-Pierre
author_facet Gaulier, Geoffrey
Dietschi, Quentin
Bhattacharyya, Swarnendu
Schmidt, Cédric
Montagnese, Matteo
Chauvet, Adrien
Hermelin, Sylvain
Chiodini, Florence
Bonacina, Luigi
Herrera, Pedro L.
Rothlisberger, Ursula
Rodriguez, Ivan
Wolf, Jean-Pierre
author_sort Gaulier, Geoffrey
collection PubMed
description Vision is usually assumed to be sensitive to the light intensity and spectrum but not to its spectral phase. However, experiments performed on retinal proteins in solution showed that the first step of vision consists in an ultrafast photoisomerization that can be coherently controlled by shaping the phase of femtosecond laser pulses, especially in the multiphoton interaction regime. The link between these experiments in solution and the biological process allowing vision was not demonstrated. Here, we measure the electric signals fired from the retina of living mice upon femtosecond multipulse and single-pulse light stimulation. Our results show that the electrophysiological signaling is sensitive to the manipulation of the light excitation on a femtosecond time scale. The mechanism relies on multiple interactions with the light pulses close to the conical intersection, like pump-dump (photoisomerization interruption) and pump-repump (reverse isomerization) processes. This interpretation is supported both experimentally and by dynamics simulations.
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spelling pubmed-80813672021-05-13 Ultrafast pulse shaping modulates perceived visual brightness in living animals Gaulier, Geoffrey Dietschi, Quentin Bhattacharyya, Swarnendu Schmidt, Cédric Montagnese, Matteo Chauvet, Adrien Hermelin, Sylvain Chiodini, Florence Bonacina, Luigi Herrera, Pedro L. Rothlisberger, Ursula Rodriguez, Ivan Wolf, Jean-Pierre Sci Adv Research Articles Vision is usually assumed to be sensitive to the light intensity and spectrum but not to its spectral phase. However, experiments performed on retinal proteins in solution showed that the first step of vision consists in an ultrafast photoisomerization that can be coherently controlled by shaping the phase of femtosecond laser pulses, especially in the multiphoton interaction regime. The link between these experiments in solution and the biological process allowing vision was not demonstrated. Here, we measure the electric signals fired from the retina of living mice upon femtosecond multipulse and single-pulse light stimulation. Our results show that the electrophysiological signaling is sensitive to the manipulation of the light excitation on a femtosecond time scale. The mechanism relies on multiple interactions with the light pulses close to the conical intersection, like pump-dump (photoisomerization interruption) and pump-repump (reverse isomerization) processes. This interpretation is supported both experimentally and by dynamics simulations. American Association for the Advancement of Science 2021-04-28 /pmc/articles/PMC8081367/ /pubmed/33910906 http://dx.doi.org/10.1126/sciadv.abe1911 Text en Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Research Articles
Gaulier, Geoffrey
Dietschi, Quentin
Bhattacharyya, Swarnendu
Schmidt, Cédric
Montagnese, Matteo
Chauvet, Adrien
Hermelin, Sylvain
Chiodini, Florence
Bonacina, Luigi
Herrera, Pedro L.
Rothlisberger, Ursula
Rodriguez, Ivan
Wolf, Jean-Pierre
Ultrafast pulse shaping modulates perceived visual brightness in living animals
title Ultrafast pulse shaping modulates perceived visual brightness in living animals
title_full Ultrafast pulse shaping modulates perceived visual brightness in living animals
title_fullStr Ultrafast pulse shaping modulates perceived visual brightness in living animals
title_full_unstemmed Ultrafast pulse shaping modulates perceived visual brightness in living animals
title_short Ultrafast pulse shaping modulates perceived visual brightness in living animals
title_sort ultrafast pulse shaping modulates perceived visual brightness in living animals
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081367/
https://www.ncbi.nlm.nih.gov/pubmed/33910906
http://dx.doi.org/10.1126/sciadv.abe1911
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