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Sensitivity of ID NOW and RT–PCR for detection of SARS-CoV-2 in an ambulatory population

Diagnosis of SARS-CoV-2 (COVID-19) requires confirmation by reverse transcription–polymerase chain reaction (RT–PCR). Abbott ID NOW provides fast results but has been criticized for low sensitivity. Here we determine the sensitivity of ID NOW in an ambulatory population presented for testing. The st...

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Autores principales: Tu, Yuan-Po, Iqbal, Jameel, O'Leary, Timothy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081522/
https://www.ncbi.nlm.nih.gov/pubmed/33876726
http://dx.doi.org/10.7554/eLife.65726
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author Tu, Yuan-Po
Iqbal, Jameel
O'Leary, Timothy
author_facet Tu, Yuan-Po
Iqbal, Jameel
O'Leary, Timothy
author_sort Tu, Yuan-Po
collection PubMed
description Diagnosis of SARS-CoV-2 (COVID-19) requires confirmation by reverse transcription–polymerase chain reaction (RT–PCR). Abbott ID NOW provides fast results but has been criticized for low sensitivity. Here we determine the sensitivity of ID NOW in an ambulatory population presented for testing. The study enrolled 785 symptomatic patients, of whom 21 were positive by both ID NOW and RT–PCR, and 2 only by RT–PCR. All 189 asymptomatic patients tested negative. The positive percent agreement between the ID NOW assay and the RT–PCR assay was 91.3%, and negative percent agreement was 100%. The results from the current study were included into a larger systematic review of literature where at least 20 subjects were simultaneously tested using ID NOW and RT–PCR. The overall sensitivity for ID NOW assay was calculated at 84% (95% confidence interval 55–96%) and had the highest correlation to RT–PCR at viral loads most likely to be associated with transmissible infections.
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spelling pubmed-80815222021-04-30 Sensitivity of ID NOW and RT–PCR for detection of SARS-CoV-2 in an ambulatory population Tu, Yuan-Po Iqbal, Jameel O'Leary, Timothy eLife Medicine Diagnosis of SARS-CoV-2 (COVID-19) requires confirmation by reverse transcription–polymerase chain reaction (RT–PCR). Abbott ID NOW provides fast results but has been criticized for low sensitivity. Here we determine the sensitivity of ID NOW in an ambulatory population presented for testing. The study enrolled 785 symptomatic patients, of whom 21 were positive by both ID NOW and RT–PCR, and 2 only by RT–PCR. All 189 asymptomatic patients tested negative. The positive percent agreement between the ID NOW assay and the RT–PCR assay was 91.3%, and negative percent agreement was 100%. The results from the current study were included into a larger systematic review of literature where at least 20 subjects were simultaneously tested using ID NOW and RT–PCR. The overall sensitivity for ID NOW assay was calculated at 84% (95% confidence interval 55–96%) and had the highest correlation to RT–PCR at viral loads most likely to be associated with transmissible infections. eLife Sciences Publications, Ltd 2021-04-20 /pmc/articles/PMC8081522/ /pubmed/33876726 http://dx.doi.org/10.7554/eLife.65726 Text en https://creativecommons.org/publicdomain/zero/1.0/This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication (https://creativecommons.org/publicdomain/zero/1.0/) .
spellingShingle Medicine
Tu, Yuan-Po
Iqbal, Jameel
O'Leary, Timothy
Sensitivity of ID NOW and RT–PCR for detection of SARS-CoV-2 in an ambulatory population
title Sensitivity of ID NOW and RT–PCR for detection of SARS-CoV-2 in an ambulatory population
title_full Sensitivity of ID NOW and RT–PCR for detection of SARS-CoV-2 in an ambulatory population
title_fullStr Sensitivity of ID NOW and RT–PCR for detection of SARS-CoV-2 in an ambulatory population
title_full_unstemmed Sensitivity of ID NOW and RT–PCR for detection of SARS-CoV-2 in an ambulatory population
title_short Sensitivity of ID NOW and RT–PCR for detection of SARS-CoV-2 in an ambulatory population
title_sort sensitivity of id now and rt–pcr for detection of sars-cov-2 in an ambulatory population
topic Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081522/
https://www.ncbi.nlm.nih.gov/pubmed/33876726
http://dx.doi.org/10.7554/eLife.65726
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