Cargando…

Symbiotic Interaction Enhances the Recovery of Endangered Tree Species in the Fragmented Maulino Forest

Beneficial plant-associated microorganisms, such as fungal endophytes, are key partners that normally improve plant survival under different environmental stresses. It has been shown that microorganisms from extreme environments, like those associated with the roots of Antarctica plants, can be good...

Descripción completa

Detalles Bibliográficos
Autores principales: Torres-Díaz, Cristian, Valladares, Moisés A., Acuña-Rodríguez, Ian S., Ballesteros, Gabriel I., Barrera, Andrea, Atala, Cristian, Molina-Montenegro, Marco A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081837/
https://www.ncbi.nlm.nih.gov/pubmed/33936153
http://dx.doi.org/10.3389/fpls.2021.663017
_version_ 1783685715819757568
author Torres-Díaz, Cristian
Valladares, Moisés A.
Acuña-Rodríguez, Ian S.
Ballesteros, Gabriel I.
Barrera, Andrea
Atala, Cristian
Molina-Montenegro, Marco A.
author_facet Torres-Díaz, Cristian
Valladares, Moisés A.
Acuña-Rodríguez, Ian S.
Ballesteros, Gabriel I.
Barrera, Andrea
Atala, Cristian
Molina-Montenegro, Marco A.
author_sort Torres-Díaz, Cristian
collection PubMed
description Beneficial plant-associated microorganisms, such as fungal endophytes, are key partners that normally improve plant survival under different environmental stresses. It has been shown that microorganisms from extreme environments, like those associated with the roots of Antarctica plants, can be good partners to increase the performance of crop plants and to restore endangered native plants. Nothofagus alessandrii and N. glauca, are among the most endangered species of Chile, restricted to a narrow and/or limited distributional range associated mainly to the Maulino forest in Chile. Here we evaluated the effect of the inoculation with a fungal consortium of root endophytes isolated from the Antarctic host plant Colobanthus quitensis on the ecophysiological performance [photosynthesis, water use efficiency (WUE), and growth] of both endangered tree species. We also, tested how Antarctic root-fungal endophytes could affect the potential distribution of N. alessandrii through niche modeling. Additionally, we conducted a transplant experiment recording plant survival on 2 years in order to validate the model. Lastly, to evaluate if inoculation with Antarctic endophytes has negative impacts on native soil microorganisms, we compared the biodiversity of fungi and bacterial in the rhizospheric soil of transplanted individuals of N. alessandrii inoculated and non-inoculated with fungal endophytes. We found that inoculation with root-endophytes produced significant increases in N. alessandrii and N. glauca photosynthetic rates, water use efficiencies and cumulative growth. In N. alessandrii, seedling survival was significantly greater on inoculated plants compared with non-inoculated individuals. For this species, a spatial distribution modeling revealed that, inoculation with root-fungal endophytes could potentially increase the current distributional range by almost threefold. Inoculation with root-fungal endophytes, did not reduce native rhizospheric microbiome diversity. Our results suggest that the studied consortium of Antarctic root-fungal endophytes improve the ecophysiological performance as well as the survival of inoculated trees and can be used as a biotechnological tool for the restoration of endangered tree species.
format Online
Article
Text
id pubmed-8081837
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-80818372021-04-30 Symbiotic Interaction Enhances the Recovery of Endangered Tree Species in the Fragmented Maulino Forest Torres-Díaz, Cristian Valladares, Moisés A. Acuña-Rodríguez, Ian S. Ballesteros, Gabriel I. Barrera, Andrea Atala, Cristian Molina-Montenegro, Marco A. Front Plant Sci Plant Science Beneficial plant-associated microorganisms, such as fungal endophytes, are key partners that normally improve plant survival under different environmental stresses. It has been shown that microorganisms from extreme environments, like those associated with the roots of Antarctica plants, can be good partners to increase the performance of crop plants and to restore endangered native plants. Nothofagus alessandrii and N. glauca, are among the most endangered species of Chile, restricted to a narrow and/or limited distributional range associated mainly to the Maulino forest in Chile. Here we evaluated the effect of the inoculation with a fungal consortium of root endophytes isolated from the Antarctic host plant Colobanthus quitensis on the ecophysiological performance [photosynthesis, water use efficiency (WUE), and growth] of both endangered tree species. We also, tested how Antarctic root-fungal endophytes could affect the potential distribution of N. alessandrii through niche modeling. Additionally, we conducted a transplant experiment recording plant survival on 2 years in order to validate the model. Lastly, to evaluate if inoculation with Antarctic endophytes has negative impacts on native soil microorganisms, we compared the biodiversity of fungi and bacterial in the rhizospheric soil of transplanted individuals of N. alessandrii inoculated and non-inoculated with fungal endophytes. We found that inoculation with root-endophytes produced significant increases in N. alessandrii and N. glauca photosynthetic rates, water use efficiencies and cumulative growth. In N. alessandrii, seedling survival was significantly greater on inoculated plants compared with non-inoculated individuals. For this species, a spatial distribution modeling revealed that, inoculation with root-fungal endophytes could potentially increase the current distributional range by almost threefold. Inoculation with root-fungal endophytes, did not reduce native rhizospheric microbiome diversity. Our results suggest that the studied consortium of Antarctic root-fungal endophytes improve the ecophysiological performance as well as the survival of inoculated trees and can be used as a biotechnological tool for the restoration of endangered tree species. Frontiers Media S.A. 2021-04-15 /pmc/articles/PMC8081837/ /pubmed/33936153 http://dx.doi.org/10.3389/fpls.2021.663017 Text en Copyright © 2021 Torres-Díaz, Valladares, Acuña-Rodríguez, Ballesteros, Barrera, Atala and Molina-Montenegro. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Torres-Díaz, Cristian
Valladares, Moisés A.
Acuña-Rodríguez, Ian S.
Ballesteros, Gabriel I.
Barrera, Andrea
Atala, Cristian
Molina-Montenegro, Marco A.
Symbiotic Interaction Enhances the Recovery of Endangered Tree Species in the Fragmented Maulino Forest
title Symbiotic Interaction Enhances the Recovery of Endangered Tree Species in the Fragmented Maulino Forest
title_full Symbiotic Interaction Enhances the Recovery of Endangered Tree Species in the Fragmented Maulino Forest
title_fullStr Symbiotic Interaction Enhances the Recovery of Endangered Tree Species in the Fragmented Maulino Forest
title_full_unstemmed Symbiotic Interaction Enhances the Recovery of Endangered Tree Species in the Fragmented Maulino Forest
title_short Symbiotic Interaction Enhances the Recovery of Endangered Tree Species in the Fragmented Maulino Forest
title_sort symbiotic interaction enhances the recovery of endangered tree species in the fragmented maulino forest
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081837/
https://www.ncbi.nlm.nih.gov/pubmed/33936153
http://dx.doi.org/10.3389/fpls.2021.663017
work_keys_str_mv AT torresdiazcristian symbioticinteractionenhancestherecoveryofendangeredtreespeciesinthefragmentedmaulinoforest
AT valladaresmoisesa symbioticinteractionenhancestherecoveryofendangeredtreespeciesinthefragmentedmaulinoforest
AT acunarodriguezians symbioticinteractionenhancestherecoveryofendangeredtreespeciesinthefragmentedmaulinoforest
AT ballesterosgabrieli symbioticinteractionenhancestherecoveryofendangeredtreespeciesinthefragmentedmaulinoforest
AT barreraandrea symbioticinteractionenhancestherecoveryofendangeredtreespeciesinthefragmentedmaulinoforest
AT atalacristian symbioticinteractionenhancestherecoveryofendangeredtreespeciesinthefragmentedmaulinoforest
AT molinamontenegromarcoa symbioticinteractionenhancestherecoveryofendangeredtreespeciesinthefragmentedmaulinoforest