Cargando…

外周血样本的存储和运输对BCR-ABL(P210)转录本水平定量检测结果的影响

OBJECTIVE: To explore the influence of storage and delivery conditions of the peripheral blood samples from patients with chronic myeloid leukemia(CML)on the real-time quantitative PCR(RQ-PCR)detection of the BCR-ABL(P210)transcript levels. METHODS: The peripheral blood samples of 84 CML patients we...

Descripción completa

Detalles Bibliográficos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Editorial office of Chinese Journal of Hematology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081946/
https://www.ncbi.nlm.nih.gov/pubmed/33910308
http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2021.03.008
_version_ 1783685743547252736
collection PubMed
description OBJECTIVE: To explore the influence of storage and delivery conditions of the peripheral blood samples from patients with chronic myeloid leukemia(CML)on the real-time quantitative PCR(RQ-PCR)detection of the BCR-ABL(P210)transcript levels. METHODS: The peripheral blood samples of 84 CML patients were collected. The same sample was divided into different groups according to storage time(0, 6, 12, 24, 48, and 72 h), temperature(room temperature, 18–24 °C; low temperature, 2–8 °C), and vibration conditions(3, 6, and 12 h). RQ-PCR was used to detect BCR-ABL(P210)transcript levels of the different groups. This study logarithmically transformed(log(10)(N))the original data[BCR-ABL copy number, ABL copy number, and BCR-ABL(P210)transcript levels]. RESULTS: ① Agarose gel electrophoresis showed significant RNA degradation of samples after storage for 48 and 72 h at room temperature. ②Among the overall samples, the BCR-ABL copy number of the samples stored at room temperature for 48 and 72 h was significantly lower than that of the samples stored at low temperature(P<0.05). However, the BCR-ABL(P210)transcript levels had no significant difference between samples stored at low temperature and room temperature. ③No significant changes were noted in the BCR-ABL(P210)transcript levels at different storage times(6, 12, 24, 48, and 72 h)regardless of storage temperature(P>0.05)compared with that at baseline(0 h, −0.56±1.51). ④ The BCR-ABL copy number of the overall sample only decreased significantly(P<0.05)at 48 h(2.93±1.59)and 72 h(2.79±1.42)compared with that at baseline(0 h, 3.35±1.60)when stored at room temperature. The ABL copy number in the overall sample decreased significantly at 48 and 72 h(whether low and room temperature; P<0.05). However, no significant changes were noted in the BCR-ABL(P210)transcript levels after vibration for 3 h(−1.29±1.81), 6 h(−1.24±1.72), and 12 h(−1.18±1.68; P>0.05)compared with that at baseline(0 h, −0.60±1.37). CONCLUSION: Sample storage time, storage temperature, and vibration can interfere with the results of BCR-ABL and ABL copy number but have no significant effect on the quantitative determination of BCR-ABL(P210)transcript levels. This study provides strong support for the feasibility of transregional transportation of peripheral blood samples from patients with CML.
format Online
Article
Text
id pubmed-8081946
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Editorial office of Chinese Journal of Hematology
record_format MEDLINE/PubMed
spelling pubmed-80819462021-04-29 外周血样本的存储和运输对BCR-ABL(P210)转录本水平定量检测结果的影响 Zhonghua Xue Ye Xue Za Zhi 论著 OBJECTIVE: To explore the influence of storage and delivery conditions of the peripheral blood samples from patients with chronic myeloid leukemia(CML)on the real-time quantitative PCR(RQ-PCR)detection of the BCR-ABL(P210)transcript levels. METHODS: The peripheral blood samples of 84 CML patients were collected. The same sample was divided into different groups according to storage time(0, 6, 12, 24, 48, and 72 h), temperature(room temperature, 18–24 °C; low temperature, 2–8 °C), and vibration conditions(3, 6, and 12 h). RQ-PCR was used to detect BCR-ABL(P210)transcript levels of the different groups. This study logarithmically transformed(log(10)(N))the original data[BCR-ABL copy number, ABL copy number, and BCR-ABL(P210)transcript levels]. RESULTS: ① Agarose gel electrophoresis showed significant RNA degradation of samples after storage for 48 and 72 h at room temperature. ②Among the overall samples, the BCR-ABL copy number of the samples stored at room temperature for 48 and 72 h was significantly lower than that of the samples stored at low temperature(P<0.05). However, the BCR-ABL(P210)transcript levels had no significant difference between samples stored at low temperature and room temperature. ③No significant changes were noted in the BCR-ABL(P210)transcript levels at different storage times(6, 12, 24, 48, and 72 h)regardless of storage temperature(P>0.05)compared with that at baseline(0 h, −0.56±1.51). ④ The BCR-ABL copy number of the overall sample only decreased significantly(P<0.05)at 48 h(2.93±1.59)and 72 h(2.79±1.42)compared with that at baseline(0 h, 3.35±1.60)when stored at room temperature. The ABL copy number in the overall sample decreased significantly at 48 and 72 h(whether low and room temperature; P<0.05). However, no significant changes were noted in the BCR-ABL(P210)transcript levels after vibration for 3 h(−1.29±1.81), 6 h(−1.24±1.72), and 12 h(−1.18±1.68; P>0.05)compared with that at baseline(0 h, −0.60±1.37). CONCLUSION: Sample storage time, storage temperature, and vibration can interfere with the results of BCR-ABL and ABL copy number but have no significant effect on the quantitative determination of BCR-ABL(P210)transcript levels. This study provides strong support for the feasibility of transregional transportation of peripheral blood samples from patients with CML. Editorial office of Chinese Journal of Hematology 2021-03 /pmc/articles/PMC8081946/ /pubmed/33910308 http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2021.03.008 Text en 2021年版权归中华医学会所有 https://creativecommons.org/licenses/by-nc-sa/3.0/This work is licensed under a Creative Commons Attribution 3.0 License (CC-BY-NC). The Copyright own by Publisher. Without authorization, shall not reprint, except this publication article, shall not use this publication format design. Unless otherwise stated, all articles published in this journal do not represent the views of the Chinese Medical Association or the editorial board of this journal.
spellingShingle 论著
外周血样本的存储和运输对BCR-ABL(P210)转录本水平定量检测结果的影响
title 外周血样本的存储和运输对BCR-ABL(P210)转录本水平定量检测结果的影响
title_full 外周血样本的存储和运输对BCR-ABL(P210)转录本水平定量检测结果的影响
title_fullStr 外周血样本的存储和运输对BCR-ABL(P210)转录本水平定量检测结果的影响
title_full_unstemmed 外周血样本的存储和运输对BCR-ABL(P210)转录本水平定量检测结果的影响
title_short 外周血样本的存储和运输对BCR-ABL(P210)转录本水平定量检测结果的影响
title_sort 外周血样本的存储和运输对bcr-abl(p210)转录本水平定量检测结果的影响
topic 论著
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081946/
https://www.ncbi.nlm.nih.gov/pubmed/33910308
http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2021.03.008
work_keys_str_mv AT wàizhōuxuèyàngběndecúnchǔhéyùnshūduìbcrablp210zhuǎnlùběnshuǐpíngdìngliàngjiǎncèjiéguǒdeyǐngxiǎng
AT wàizhōuxuèyàngběndecúnchǔhéyùnshūduìbcrablp210zhuǎnlùběnshuǐpíngdìngliàngjiǎncèjiéguǒdeyǐngxiǎng
AT wàizhōuxuèyàngběndecúnchǔhéyùnshūduìbcrablp210zhuǎnlùběnshuǐpíngdìngliàngjiǎncèjiéguǒdeyǐngxiǎng
AT wàizhōuxuèyàngběndecúnchǔhéyùnshūduìbcrablp210zhuǎnlùběnshuǐpíngdìngliàngjiǎncèjiéguǒdeyǐngxiǎng
AT wàizhōuxuèyàngběndecúnchǔhéyùnshūduìbcrablp210zhuǎnlùběnshuǐpíngdìngliàngjiǎncèjiéguǒdeyǐngxiǎng
AT wàizhōuxuèyàngběndecúnchǔhéyùnshūduìbcrablp210zhuǎnlùběnshuǐpíngdìngliàngjiǎncèjiéguǒdeyǐngxiǎng
AT wàizhōuxuèyàngběndecúnchǔhéyùnshūduìbcrablp210zhuǎnlùběnshuǐpíngdìngliàngjiǎncèjiéguǒdeyǐngxiǎng
AT wàizhōuxuèyàngběndecúnchǔhéyùnshūduìbcrablp210zhuǎnlùběnshuǐpíngdìngliàngjiǎncèjiéguǒdeyǐngxiǎng
AT wàizhōuxuèyàngběndecúnchǔhéyùnshūduìbcrablp210zhuǎnlùběnshuǐpíngdìngliàngjiǎncèjiéguǒdeyǐngxiǎng