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外周血样本的存储和运输对BCR-ABL(P210)转录本水平定量检测结果的影响
OBJECTIVE: To explore the influence of storage and delivery conditions of the peripheral blood samples from patients with chronic myeloid leukemia(CML)on the real-time quantitative PCR(RQ-PCR)detection of the BCR-ABL(P210)transcript levels. METHODS: The peripheral blood samples of 84 CML patients we...
Formato: | Online Artículo Texto |
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Lenguaje: | English |
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Editorial office of Chinese Journal of Hematology
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081946/ https://www.ncbi.nlm.nih.gov/pubmed/33910308 http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2021.03.008 |
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collection | PubMed |
description | OBJECTIVE: To explore the influence of storage and delivery conditions of the peripheral blood samples from patients with chronic myeloid leukemia(CML)on the real-time quantitative PCR(RQ-PCR)detection of the BCR-ABL(P210)transcript levels. METHODS: The peripheral blood samples of 84 CML patients were collected. The same sample was divided into different groups according to storage time(0, 6, 12, 24, 48, and 72 h), temperature(room temperature, 18–24 °C; low temperature, 2–8 °C), and vibration conditions(3, 6, and 12 h). RQ-PCR was used to detect BCR-ABL(P210)transcript levels of the different groups. This study logarithmically transformed(log(10)(N))the original data[BCR-ABL copy number, ABL copy number, and BCR-ABL(P210)transcript levels]. RESULTS: ① Agarose gel electrophoresis showed significant RNA degradation of samples after storage for 48 and 72 h at room temperature. ②Among the overall samples, the BCR-ABL copy number of the samples stored at room temperature for 48 and 72 h was significantly lower than that of the samples stored at low temperature(P<0.05). However, the BCR-ABL(P210)transcript levels had no significant difference between samples stored at low temperature and room temperature. ③No significant changes were noted in the BCR-ABL(P210)transcript levels at different storage times(6, 12, 24, 48, and 72 h)regardless of storage temperature(P>0.05)compared with that at baseline(0 h, −0.56±1.51). ④ The BCR-ABL copy number of the overall sample only decreased significantly(P<0.05)at 48 h(2.93±1.59)and 72 h(2.79±1.42)compared with that at baseline(0 h, 3.35±1.60)when stored at room temperature. The ABL copy number in the overall sample decreased significantly at 48 and 72 h(whether low and room temperature; P<0.05). However, no significant changes were noted in the BCR-ABL(P210)transcript levels after vibration for 3 h(−1.29±1.81), 6 h(−1.24±1.72), and 12 h(−1.18±1.68; P>0.05)compared with that at baseline(0 h, −0.60±1.37). CONCLUSION: Sample storage time, storage temperature, and vibration can interfere with the results of BCR-ABL and ABL copy number but have no significant effect on the quantitative determination of BCR-ABL(P210)transcript levels. This study provides strong support for the feasibility of transregional transportation of peripheral blood samples from patients with CML. |
format | Online Article Text |
id | pubmed-8081946 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Editorial office of Chinese Journal of Hematology |
record_format | MEDLINE/PubMed |
spelling | pubmed-80819462021-04-29 外周血样本的存储和运输对BCR-ABL(P210)转录本水平定量检测结果的影响 Zhonghua Xue Ye Xue Za Zhi 论著 OBJECTIVE: To explore the influence of storage and delivery conditions of the peripheral blood samples from patients with chronic myeloid leukemia(CML)on the real-time quantitative PCR(RQ-PCR)detection of the BCR-ABL(P210)transcript levels. METHODS: The peripheral blood samples of 84 CML patients were collected. The same sample was divided into different groups according to storage time(0, 6, 12, 24, 48, and 72 h), temperature(room temperature, 18–24 °C; low temperature, 2–8 °C), and vibration conditions(3, 6, and 12 h). RQ-PCR was used to detect BCR-ABL(P210)transcript levels of the different groups. This study logarithmically transformed(log(10)(N))the original data[BCR-ABL copy number, ABL copy number, and BCR-ABL(P210)transcript levels]. RESULTS: ① Agarose gel electrophoresis showed significant RNA degradation of samples after storage for 48 and 72 h at room temperature. ②Among the overall samples, the BCR-ABL copy number of the samples stored at room temperature for 48 and 72 h was significantly lower than that of the samples stored at low temperature(P<0.05). However, the BCR-ABL(P210)transcript levels had no significant difference between samples stored at low temperature and room temperature. ③No significant changes were noted in the BCR-ABL(P210)transcript levels at different storage times(6, 12, 24, 48, and 72 h)regardless of storage temperature(P>0.05)compared with that at baseline(0 h, −0.56±1.51). ④ The BCR-ABL copy number of the overall sample only decreased significantly(P<0.05)at 48 h(2.93±1.59)and 72 h(2.79±1.42)compared with that at baseline(0 h, 3.35±1.60)when stored at room temperature. The ABL copy number in the overall sample decreased significantly at 48 and 72 h(whether low and room temperature; P<0.05). However, no significant changes were noted in the BCR-ABL(P210)transcript levels after vibration for 3 h(−1.29±1.81), 6 h(−1.24±1.72), and 12 h(−1.18±1.68; P>0.05)compared with that at baseline(0 h, −0.60±1.37). CONCLUSION: Sample storage time, storage temperature, and vibration can interfere with the results of BCR-ABL and ABL copy number but have no significant effect on the quantitative determination of BCR-ABL(P210)transcript levels. This study provides strong support for the feasibility of transregional transportation of peripheral blood samples from patients with CML. Editorial office of Chinese Journal of Hematology 2021-03 /pmc/articles/PMC8081946/ /pubmed/33910308 http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2021.03.008 Text en 2021年版权归中华医学会所有 https://creativecommons.org/licenses/by-nc-sa/3.0/This work is licensed under a Creative Commons Attribution 3.0 License (CC-BY-NC). The Copyright own by Publisher. Without authorization, shall not reprint, except this publication article, shall not use this publication format design. Unless otherwise stated, all articles published in this journal do not represent the views of the Chinese Medical Association or the editorial board of this journal. |
spellingShingle | 论著 外周血样本的存储和运输对BCR-ABL(P210)转录本水平定量检测结果的影响 |
title | 外周血样本的存储和运输对BCR-ABL(P210)转录本水平定量检测结果的影响 |
title_full | 外周血样本的存储和运输对BCR-ABL(P210)转录本水平定量检测结果的影响 |
title_fullStr | 外周血样本的存储和运输对BCR-ABL(P210)转录本水平定量检测结果的影响 |
title_full_unstemmed | 外周血样本的存储和运输对BCR-ABL(P210)转录本水平定量检测结果的影响 |
title_short | 外周血样本的存储和运输对BCR-ABL(P210)转录本水平定量检测结果的影响 |
title_sort | 外周血样本的存储和运输对bcr-abl(p210)转录本水平定量检测结果的影响 |
topic | 论著 |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081946/ https://www.ncbi.nlm.nih.gov/pubmed/33910308 http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2021.03.008 |
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