Cargando…
Two Small Extracellular Vesicle sRNAs Derived From Mycobacterium tuberculosis Serve as Diagnostic Biomarkers for Active Pulmonary Tuberculosis
The rapid diagnosis of tuberculosis (TB) is of great significance for the control and treatment of TB. However, TB remains a major healthy, social, and economic burden worldwide because of the lack of ideal diagnostic biomarkers. Mycobacterium tuberculosis (M. tuberculosis)-encoded small RNA (sRNA)...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082067/ https://www.ncbi.nlm.nih.gov/pubmed/33936000 http://dx.doi.org/10.3389/fmicb.2021.642559 |
_version_ | 1783685772218466304 |
---|---|
author | Lu, Geng Jiang, XinRui Wu, Anni Zhou, Jiawei Liu, Hengjun He, Fei Zhang, Qiuling Zen, Ke Gu, Shuangshuang Wang, Jun |
author_facet | Lu, Geng Jiang, XinRui Wu, Anni Zhou, Jiawei Liu, Hengjun He, Fei Zhang, Qiuling Zen, Ke Gu, Shuangshuang Wang, Jun |
author_sort | Lu, Geng |
collection | PubMed |
description | The rapid diagnosis of tuberculosis (TB) is of great significance for the control and treatment of TB. However, TB remains a major healthy, social, and economic burden worldwide because of the lack of ideal diagnostic biomarkers. Mycobacterium tuberculosis (M. tuberculosis)-encoded small RNA (sRNA) is a class of regulation small RNA. Several studies have identified M. tuberculosis encoded-sRNAs in the serum/plasm of M. tuberculosis-infected patients. Small extracellular vesicles are small membrane vesicles secreted by many cell types during physiological and pathological conditions. Recent evidence has indicated that most of the nucleic acids in the serum/plasma are packaged in the small extracellular vesicles and could serve as ideal diagnostic biomarkers. In this study, we attempted a novel approach for TB diagnosis: targeting small extracellular vesicles M. tuberculosis encoded sRNA (sRNA) by qRT-PCR. The results showed that M. tuberculosis-encoded ASdes and MTB-miR5 only existed in tuberculosis patients and have the potential to serve as a sensitive and accurate methodology for TB diagnosis. |
format | Online Article Text |
id | pubmed-8082067 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-80820672021-04-30 Two Small Extracellular Vesicle sRNAs Derived From Mycobacterium tuberculosis Serve as Diagnostic Biomarkers for Active Pulmonary Tuberculosis Lu, Geng Jiang, XinRui Wu, Anni Zhou, Jiawei Liu, Hengjun He, Fei Zhang, Qiuling Zen, Ke Gu, Shuangshuang Wang, Jun Front Microbiol Microbiology The rapid diagnosis of tuberculosis (TB) is of great significance for the control and treatment of TB. However, TB remains a major healthy, social, and economic burden worldwide because of the lack of ideal diagnostic biomarkers. Mycobacterium tuberculosis (M. tuberculosis)-encoded small RNA (sRNA) is a class of regulation small RNA. Several studies have identified M. tuberculosis encoded-sRNAs in the serum/plasm of M. tuberculosis-infected patients. Small extracellular vesicles are small membrane vesicles secreted by many cell types during physiological and pathological conditions. Recent evidence has indicated that most of the nucleic acids in the serum/plasma are packaged in the small extracellular vesicles and could serve as ideal diagnostic biomarkers. In this study, we attempted a novel approach for TB diagnosis: targeting small extracellular vesicles M. tuberculosis encoded sRNA (sRNA) by qRT-PCR. The results showed that M. tuberculosis-encoded ASdes and MTB-miR5 only existed in tuberculosis patients and have the potential to serve as a sensitive and accurate methodology for TB diagnosis. Frontiers Media S.A. 2021-04-15 /pmc/articles/PMC8082067/ /pubmed/33936000 http://dx.doi.org/10.3389/fmicb.2021.642559 Text en Copyright © 2021 Lu, Jiang, Wu, Zhou, Liu, He, Zhang, Zen, Gu and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Lu, Geng Jiang, XinRui Wu, Anni Zhou, Jiawei Liu, Hengjun He, Fei Zhang, Qiuling Zen, Ke Gu, Shuangshuang Wang, Jun Two Small Extracellular Vesicle sRNAs Derived From Mycobacterium tuberculosis Serve as Diagnostic Biomarkers for Active Pulmonary Tuberculosis |
title | Two Small Extracellular Vesicle sRNAs Derived From Mycobacterium tuberculosis Serve as Diagnostic Biomarkers for Active Pulmonary Tuberculosis |
title_full | Two Small Extracellular Vesicle sRNAs Derived From Mycobacterium tuberculosis Serve as Diagnostic Biomarkers for Active Pulmonary Tuberculosis |
title_fullStr | Two Small Extracellular Vesicle sRNAs Derived From Mycobacterium tuberculosis Serve as Diagnostic Biomarkers for Active Pulmonary Tuberculosis |
title_full_unstemmed | Two Small Extracellular Vesicle sRNAs Derived From Mycobacterium tuberculosis Serve as Diagnostic Biomarkers for Active Pulmonary Tuberculosis |
title_short | Two Small Extracellular Vesicle sRNAs Derived From Mycobacterium tuberculosis Serve as Diagnostic Biomarkers for Active Pulmonary Tuberculosis |
title_sort | two small extracellular vesicle srnas derived from mycobacterium tuberculosis serve as diagnostic biomarkers for active pulmonary tuberculosis |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082067/ https://www.ncbi.nlm.nih.gov/pubmed/33936000 http://dx.doi.org/10.3389/fmicb.2021.642559 |
work_keys_str_mv | AT lugeng twosmallextracellularvesiclesrnasderivedfrommycobacteriumtuberculosisserveasdiagnosticbiomarkersforactivepulmonarytuberculosis AT jiangxinrui twosmallextracellularvesiclesrnasderivedfrommycobacteriumtuberculosisserveasdiagnosticbiomarkersforactivepulmonarytuberculosis AT wuanni twosmallextracellularvesiclesrnasderivedfrommycobacteriumtuberculosisserveasdiagnosticbiomarkersforactivepulmonarytuberculosis AT zhoujiawei twosmallextracellularvesiclesrnasderivedfrommycobacteriumtuberculosisserveasdiagnosticbiomarkersforactivepulmonarytuberculosis AT liuhengjun twosmallextracellularvesiclesrnasderivedfrommycobacteriumtuberculosisserveasdiagnosticbiomarkersforactivepulmonarytuberculosis AT hefei twosmallextracellularvesiclesrnasderivedfrommycobacteriumtuberculosisserveasdiagnosticbiomarkersforactivepulmonarytuberculosis AT zhangqiuling twosmallextracellularvesiclesrnasderivedfrommycobacteriumtuberculosisserveasdiagnosticbiomarkersforactivepulmonarytuberculosis AT zenke twosmallextracellularvesiclesrnasderivedfrommycobacteriumtuberculosisserveasdiagnosticbiomarkersforactivepulmonarytuberculosis AT gushuangshuang twosmallextracellularvesiclesrnasderivedfrommycobacteriumtuberculosisserveasdiagnosticbiomarkersforactivepulmonarytuberculosis AT wangjun twosmallextracellularvesiclesrnasderivedfrommycobacteriumtuberculosisserveasdiagnosticbiomarkersforactivepulmonarytuberculosis |