Creation of a functional unc-11/PICALM GFP knock-in by CRISPR

unc-11 is the only C. elegans ortholog of mammalian PICALM/AP180, paralogs that play an important role in Clathrin-Mediated Endocytosis (CME) and the recycling of a subset of SNAREs, the vesicle-associated membrane proteins (VAMPs). In this publication we report the creation of a new unc-11 allele t...

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Detalles Bibliográficos
Autores principales: Gallegos, Maria, Hermes, Alex, Patra, Debjani
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2021
Materias:
Materials and Reagents
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082290/
https://www.ncbi.nlm.nih.gov/pubmed/33937722
http://dx.doi.org/10.17912/micropub.biology.000389
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author Gallegos, Maria
Hermes, Alex
Patra, Debjani
author_facet Gallegos, Maria
Hermes, Alex
Patra, Debjani
author_sort Gallegos, Maria
collection PubMed
description unc-11 is the only C. elegans ortholog of mammalian PICALM/AP180, paralogs that play an important role in Clathrin-Mediated Endocytosis (CME) and the recycling of a subset of SNAREs, the vesicle-associated membrane proteins (VAMPs). In this publication we report the creation of a new unc-11 allele that is endogenously-tagged with GFP just upstream of the stop codon. Moreover, we demonstrate that the UNC-11::GFP fusion protein functions like wild type with an expression pattern similar to UNC-11 antibody staining described previously.
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spelling pubmed-80822902021-04-30 Creation of a functional unc-11/PICALM GFP knock-in by CRISPR Gallegos, Maria Hermes, Alex Patra, Debjani MicroPubl Biol Materials and Reagents unc-11 is the only C. elegans ortholog of mammalian PICALM/AP180, paralogs that play an important role in Clathrin-Mediated Endocytosis (CME) and the recycling of a subset of SNAREs, the vesicle-associated membrane proteins (VAMPs). In this publication we report the creation of a new unc-11 allele that is endogenously-tagged with GFP just upstream of the stop codon. Moreover, we demonstrate that the UNC-11::GFP fusion protein functions like wild type with an expression pattern similar to UNC-11 antibody staining described previously. Caltech Library 2021-04-28 /pmc/articles/PMC8082290/ /pubmed/33937722 http://dx.doi.org/10.17912/micropub.biology.000389 Text en Copyright: © 2021 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Materials and Reagents
Gallegos, Maria
Hermes, Alex
Patra, Debjani
Creation of a functional unc-11/PICALM GFP knock-in by CRISPR
title Creation of a functional unc-11/PICALM GFP knock-in by CRISPR
title_full Creation of a functional unc-11/PICALM GFP knock-in by CRISPR
title_fullStr Creation of a functional unc-11/PICALM GFP knock-in by CRISPR
title_full_unstemmed Creation of a functional unc-11/PICALM GFP knock-in by CRISPR
title_short Creation of a functional unc-11/PICALM GFP knock-in by CRISPR
title_sort creation of a functional unc-11/picalm gfp knock-in by crispr
topic Materials and Reagents
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082290/
https://www.ncbi.nlm.nih.gov/pubmed/33937722
http://dx.doi.org/10.17912/micropub.biology.000389
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