Hydroxypropyl-β-Cyclodextrin-Complexed Resveratrol Enhanced Antitumor Activity in a Cervical Cancer Model: In Vivo Analysis

Trans-resveratrol (RES) exhibits a wide range of biological activities. Various methodological approaches have been established to improve the pharmacokinetic properties of RES. Moreover, additional in vivo studies are required to support clinical application. In this study, RES/HP-β-CD (RHSD) inclu...

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Autores principales: Hao, Xincai, Sun, Xiaodong, Zhu, Haizhen, Xie, Lixia, Wang, Xuanbin, Jiang, Nan, Fu, Pan, Sang, Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Pharmacology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082405/
https://www.ncbi.nlm.nih.gov/pubmed/33935691
http://dx.doi.org/10.3389/fphar.2021.573909
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author Hao, Xincai
Sun, Xiaodong
Zhu, Haizhen
Xie, Lixia
Wang, Xuanbin
Jiang, Nan
Fu, Pan
Sang, Ming
author_facet Hao, Xincai
Sun, Xiaodong
Zhu, Haizhen
Xie, Lixia
Wang, Xuanbin
Jiang, Nan
Fu, Pan
Sang, Ming
author_sort Hao, Xincai
collection PubMed
description Trans-resveratrol (RES) exhibits a wide range of biological activities. Various methodological approaches have been established to improve the pharmacokinetic properties of RES. Moreover, additional in vivo studies are required to support clinical application. In this study, RES/HP-β-CD (RHSD) inclusion complex was prepared and characterized by FTIR, PXRD, DSC and NMR data. The effect and potential mechanism of RHSD against cervical cancer were investigated in a mouse xenograft tumor model by qPCR assay, Western blot assay, and immunohistochemical assay. Results showed that RHSD significantly decreased tumor growth compared with free RES, while the effect of preventing tumor growth was more prominent in vivo. Notably, RHSD could inhibit tumor development by suppressing the expression of HPV E6 and E7 oncogenes and upregulating P53 and Rb1 protein in cervical cancer. These findings demonstrated that RHSD was safe and potential for development of a new oral administration drug to treat cervical cancer.
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spelling pubmed-80824052021-04-30 Hydroxypropyl-β-Cyclodextrin-Complexed Resveratrol Enhanced Antitumor Activity in a Cervical Cancer Model: In Vivo Analysis Hao, Xincai Sun, Xiaodong Zhu, Haizhen Xie, Lixia Wang, Xuanbin Jiang, Nan Fu, Pan Sang, Ming Front Pharmacol Pharmacology Trans-resveratrol (RES) exhibits a wide range of biological activities. Various methodological approaches have been established to improve the pharmacokinetic properties of RES. Moreover, additional in vivo studies are required to support clinical application. In this study, RES/HP-β-CD (RHSD) inclusion complex was prepared and characterized by FTIR, PXRD, DSC and NMR data. The effect and potential mechanism of RHSD against cervical cancer were investigated in a mouse xenograft tumor model by qPCR assay, Western blot assay, and immunohistochemical assay. Results showed that RHSD significantly decreased tumor growth compared with free RES, while the effect of preventing tumor growth was more prominent in vivo. Notably, RHSD could inhibit tumor development by suppressing the expression of HPV E6 and E7 oncogenes and upregulating P53 and Rb1 protein in cervical cancer. These findings demonstrated that RHSD was safe and potential for development of a new oral administration drug to treat cervical cancer. Frontiers Media S.A. 2021-04-15 /pmc/articles/PMC8082405/ /pubmed/33935691 http://dx.doi.org/10.3389/fphar.2021.573909 Text en Copyright © 2021 Hao, Sun, Zhu, Xie, Wang, Jiang, Fu and Sang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Hao, Xincai
Sun, Xiaodong
Zhu, Haizhen
Xie, Lixia
Wang, Xuanbin
Jiang, Nan
Fu, Pan
Sang, Ming
Hydroxypropyl-β-Cyclodextrin-Complexed Resveratrol Enhanced Antitumor Activity in a Cervical Cancer Model: In Vivo Analysis
title Hydroxypropyl-β-Cyclodextrin-Complexed Resveratrol Enhanced Antitumor Activity in a Cervical Cancer Model: In Vivo Analysis
title_full Hydroxypropyl-β-Cyclodextrin-Complexed Resveratrol Enhanced Antitumor Activity in a Cervical Cancer Model: In Vivo Analysis
title_fullStr Hydroxypropyl-β-Cyclodextrin-Complexed Resveratrol Enhanced Antitumor Activity in a Cervical Cancer Model: In Vivo Analysis
title_full_unstemmed Hydroxypropyl-β-Cyclodextrin-Complexed Resveratrol Enhanced Antitumor Activity in a Cervical Cancer Model: In Vivo Analysis
title_short Hydroxypropyl-β-Cyclodextrin-Complexed Resveratrol Enhanced Antitumor Activity in a Cervical Cancer Model: In Vivo Analysis
title_sort hydroxypropyl-β-cyclodextrin-complexed resveratrol enhanced antitumor activity in a cervical cancer model: in vivo analysis
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082405/
https://www.ncbi.nlm.nih.gov/pubmed/33935691
http://dx.doi.org/10.3389/fphar.2021.573909
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