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Fibrillarin-GFP Facilitates the Identification of Meiotic Competent Oocytes

The nucleolus undergoes significant functional changes and plays important roles during mammalian oocyte meiotic maturation. Fibrillarin (FBL) is the component of nucleolar small nuclear ribonucleoprotein (snRNP) particle and localizes to the dense fibrillar component (DFC) of the nucleolus. We foun...

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Autores principales: Wang, Ting, Na, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082495/
https://www.ncbi.nlm.nih.gov/pubmed/33937243
http://dx.doi.org/10.3389/fcell.2021.648331
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author Wang, Ting
Na, Jie
author_facet Wang, Ting
Na, Jie
author_sort Wang, Ting
collection PubMed
description The nucleolus undergoes significant functional changes and plays important roles during mammalian oocyte meiotic maturation. Fibrillarin (FBL) is the component of nucleolar small nuclear ribonucleoprotein (snRNP) particle and localizes to the dense fibrillar component (DFC) of the nucleolus. We found that FBL–GFP displays an uneven and cloudy localization in the nucleolus of non-surrounded nucleolus (NSN) oocytes, while it distributes evenly and to a few bright dots in the surrounded nucleolus (SN) oocytes. Accordingly, NSN oocytes showed active nascent RNA transcription, while the SN group was transcriptionally quiescent. NSN geminal vesicles also contained more DNA damage marker γH2AX foci. Based on different FBL–GFP patterns in live oocytes, the ones with superior meiotic maturation potential can be identified. Global transcriptome profiling revealed a significant difference in single SN and NSN oocytes. Thus, FBL–GFP can serve as a marker for nucleolus activity, which also correlates with transcription activity and the quality of oocytes.
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spelling pubmed-80824952021-04-30 Fibrillarin-GFP Facilitates the Identification of Meiotic Competent Oocytes Wang, Ting Na, Jie Front Cell Dev Biol Cell and Developmental Biology The nucleolus undergoes significant functional changes and plays important roles during mammalian oocyte meiotic maturation. Fibrillarin (FBL) is the component of nucleolar small nuclear ribonucleoprotein (snRNP) particle and localizes to the dense fibrillar component (DFC) of the nucleolus. We found that FBL–GFP displays an uneven and cloudy localization in the nucleolus of non-surrounded nucleolus (NSN) oocytes, while it distributes evenly and to a few bright dots in the surrounded nucleolus (SN) oocytes. Accordingly, NSN oocytes showed active nascent RNA transcription, while the SN group was transcriptionally quiescent. NSN geminal vesicles also contained more DNA damage marker γH2AX foci. Based on different FBL–GFP patterns in live oocytes, the ones with superior meiotic maturation potential can be identified. Global transcriptome profiling revealed a significant difference in single SN and NSN oocytes. Thus, FBL–GFP can serve as a marker for nucleolus activity, which also correlates with transcription activity and the quality of oocytes. Frontiers Media S.A. 2021-04-15 /pmc/articles/PMC8082495/ /pubmed/33937243 http://dx.doi.org/10.3389/fcell.2021.648331 Text en Copyright © 2021 Wang and Na. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Wang, Ting
Na, Jie
Fibrillarin-GFP Facilitates the Identification of Meiotic Competent Oocytes
title Fibrillarin-GFP Facilitates the Identification of Meiotic Competent Oocytes
title_full Fibrillarin-GFP Facilitates the Identification of Meiotic Competent Oocytes
title_fullStr Fibrillarin-GFP Facilitates the Identification of Meiotic Competent Oocytes
title_full_unstemmed Fibrillarin-GFP Facilitates the Identification of Meiotic Competent Oocytes
title_short Fibrillarin-GFP Facilitates the Identification of Meiotic Competent Oocytes
title_sort fibrillarin-gfp facilitates the identification of meiotic competent oocytes
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082495/
https://www.ncbi.nlm.nih.gov/pubmed/33937243
http://dx.doi.org/10.3389/fcell.2021.648331
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