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Local Duplication of TIR-NBS-LRR Gene Marks Clubroot Resistance in Brassica napus cv. Tosca

Clubroot, caused by Plasmodiophora brassicae infection, is a disease of growing importance in cruciferous crops, including oilseed rape (Brassica napus). The affected plants exhibit prominent galling of the roots that impairs their capacity for water and nutrient uptake, which leads to growth retard...

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Autores principales: Kopec, Piotr M., Mikolajczyk, Katarzyna, Jajor, Ewa, Perek, Agnieszka, Nowakowska, Joanna, Obermeier, Christian, Chawla, Harmeet Singh, Korbas, Marek, Bartkowiak-Broda, Iwona, Karlowski, Wojciech M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082685/
https://www.ncbi.nlm.nih.gov/pubmed/33936130
http://dx.doi.org/10.3389/fpls.2021.639631
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author Kopec, Piotr M.
Mikolajczyk, Katarzyna
Jajor, Ewa
Perek, Agnieszka
Nowakowska, Joanna
Obermeier, Christian
Chawla, Harmeet Singh
Korbas, Marek
Bartkowiak-Broda, Iwona
Karlowski, Wojciech M.
author_facet Kopec, Piotr M.
Mikolajczyk, Katarzyna
Jajor, Ewa
Perek, Agnieszka
Nowakowska, Joanna
Obermeier, Christian
Chawla, Harmeet Singh
Korbas, Marek
Bartkowiak-Broda, Iwona
Karlowski, Wojciech M.
author_sort Kopec, Piotr M.
collection PubMed
description Clubroot, caused by Plasmodiophora brassicae infection, is a disease of growing importance in cruciferous crops, including oilseed rape (Brassica napus). The affected plants exhibit prominent galling of the roots that impairs their capacity for water and nutrient uptake, which leads to growth retardation, wilting, premature ripening, or death. Due to the scarcity of effective means of protection against the pathogen, breeding of resistant varieties remains a crucial component of disease control measures. The key aspect of the breeding process is the identification of genetic factors associated with variable response to the pathogen exposure. Although numerous clubroot resistance loci have been described in Brassica crops, continuous updates on the sources of resistance are necessary. Many of the resistance genes are pathotype-specific, moreover, resistance breakdowns have been reported. In this study, we characterize the clubroot resistance locus in the winter oilseed rape cultivar “Tosca.” In a series of greenhouse experiments, we evaluate the disease severity of P. brassicae-challenged “Tosca”-derived population of doubled haploids, which we genotype with Brassica 60 K array and a selection of SSR/SCAR markers. We then construct a genetic map and narrow down the resistance locus to the 0.4 cM fragment on the A03 chromosome, corresponding to the region previously described as Crr3. Using Oxford Nanopore long-read genome resequencing and RNA-seq we review the composition of the locus and describe a duplication of TIR-NBS-LRR gene. Further, we explore the transcriptomic differences of the local genes between the clubroot resistant and susceptible, inoculated and control DH lines. We conclude that the duplicated TNL gene is a promising candidate for the resistance factor. This study provides valuable resources for clubroot resistance breeding programs and lays a foundation for further functional studies on clubroot resistance.
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spelling pubmed-80826852021-04-30 Local Duplication of TIR-NBS-LRR Gene Marks Clubroot Resistance in Brassica napus cv. Tosca Kopec, Piotr M. Mikolajczyk, Katarzyna Jajor, Ewa Perek, Agnieszka Nowakowska, Joanna Obermeier, Christian Chawla, Harmeet Singh Korbas, Marek Bartkowiak-Broda, Iwona Karlowski, Wojciech M. Front Plant Sci Plant Science Clubroot, caused by Plasmodiophora brassicae infection, is a disease of growing importance in cruciferous crops, including oilseed rape (Brassica napus). The affected plants exhibit prominent galling of the roots that impairs their capacity for water and nutrient uptake, which leads to growth retardation, wilting, premature ripening, or death. Due to the scarcity of effective means of protection against the pathogen, breeding of resistant varieties remains a crucial component of disease control measures. The key aspect of the breeding process is the identification of genetic factors associated with variable response to the pathogen exposure. Although numerous clubroot resistance loci have been described in Brassica crops, continuous updates on the sources of resistance are necessary. Many of the resistance genes are pathotype-specific, moreover, resistance breakdowns have been reported. In this study, we characterize the clubroot resistance locus in the winter oilseed rape cultivar “Tosca.” In a series of greenhouse experiments, we evaluate the disease severity of P. brassicae-challenged “Tosca”-derived population of doubled haploids, which we genotype with Brassica 60 K array and a selection of SSR/SCAR markers. We then construct a genetic map and narrow down the resistance locus to the 0.4 cM fragment on the A03 chromosome, corresponding to the region previously described as Crr3. Using Oxford Nanopore long-read genome resequencing and RNA-seq we review the composition of the locus and describe a duplication of TIR-NBS-LRR gene. Further, we explore the transcriptomic differences of the local genes between the clubroot resistant and susceptible, inoculated and control DH lines. We conclude that the duplicated TNL gene is a promising candidate for the resistance factor. This study provides valuable resources for clubroot resistance breeding programs and lays a foundation for further functional studies on clubroot resistance. Frontiers Media S.A. 2021-04-08 /pmc/articles/PMC8082685/ /pubmed/33936130 http://dx.doi.org/10.3389/fpls.2021.639631 Text en Copyright © 2021 Kopec, Mikolajczyk, Jajor, Perek, Nowakowska, Obermeier, Chawla, Korbas, Bartkowiak-Broda and Karlowski. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Kopec, Piotr M.
Mikolajczyk, Katarzyna
Jajor, Ewa
Perek, Agnieszka
Nowakowska, Joanna
Obermeier, Christian
Chawla, Harmeet Singh
Korbas, Marek
Bartkowiak-Broda, Iwona
Karlowski, Wojciech M.
Local Duplication of TIR-NBS-LRR Gene Marks Clubroot Resistance in Brassica napus cv. Tosca
title Local Duplication of TIR-NBS-LRR Gene Marks Clubroot Resistance in Brassica napus cv. Tosca
title_full Local Duplication of TIR-NBS-LRR Gene Marks Clubroot Resistance in Brassica napus cv. Tosca
title_fullStr Local Duplication of TIR-NBS-LRR Gene Marks Clubroot Resistance in Brassica napus cv. Tosca
title_full_unstemmed Local Duplication of TIR-NBS-LRR Gene Marks Clubroot Resistance in Brassica napus cv. Tosca
title_short Local Duplication of TIR-NBS-LRR Gene Marks Clubroot Resistance in Brassica napus cv. Tosca
title_sort local duplication of tir-nbs-lrr gene marks clubroot resistance in brassica napus cv. tosca
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082685/
https://www.ncbi.nlm.nih.gov/pubmed/33936130
http://dx.doi.org/10.3389/fpls.2021.639631
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