Validation and standardization of DNA extraction and library construction methods for metagenomics-based human fecal microbiome measurements

BACKGROUND: Validation and standardization of methodologies for microbial community measurements by high-throughput sequencing are needed to support human microbiome research and its industrialization. This study set out to establish standards-based solutions to improve the accuracy and reproducibil...

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Detalles Bibliográficos
Autores principales: Tourlousse, Dieter M., Narita, Koji, Miura, Takamasa, Sakamoto, Mitsuo, Ohashi, Akiko, Shiina, Keita, Matsuda, Masami, Miura, Daisuke, Shimamura, Mamiko, Ohyama, Yoshifumi, Yamazoe, Atsushi, Uchino, Yoshihito, Kameyama, Keishi, Arioka, Shingo, Kataoka, Jiro, Hisada, Takayoshi, Fujii, Kazuyuki, Takahashi, Shunsuke, Kuroiwa, Miho, Rokushima, Masatomo, Nishiyama, Mitsue, Tanaka, Yoshiki, Fuchikami, Takuya, Aoki, Hitomi, Kira, Satoshi, Koyanagi, Ryo, Naito, Takeshi, Nishiwaki, Morie, Kumagai, Hirotaka, Konda, Mikiko, Kasahara, Ken, Ohkuma, Moriya, Kawasaki, Hiroko, Sekiguchi, Yuji, Terauchi, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082873/
https://www.ncbi.nlm.nih.gov/pubmed/33910647
http://dx.doi.org/10.1186/s40168-021-01048-3

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