Morphometric analysis of basal cells of oral epithelium in predicting malignant transformation of oral potentially malignant disorders in patients with tobacco chewing habit

BACKGROUND: Oral potentially malignant disorders (OPMDs) are a heterogenous group of disorders which precede the development of oral cancer. These are characterized by increased risk of malignant transformation to Oral cancer. AIMS & OBJECTIVES: In this study, an attempt has been made to assess the morphological alterations of the nuclei of the basal cells in OPMDs and oral squamous cell carcinoma (OSCC). Our objective was to compare the alterations and to assess the predictive factor of such alterations of basal cells in malignant transformation of OPMDs to OSCC. MATERIALS AND METHODS: This was a retrospective study conducted on tissue sections of 150 formalin fixed, paraffin embedded blocks obtained from the archives. The specimens were grouped into OSCC group (n= 50) and OPMDs (n= 100). Nuclear features were evaluated using computer- assisted microscopic image analysis. One- way ANOVA analysis was done to verify the difference between the groups for all variables. RESULTS: Our results showed statistically significant difference for all parameters between the groups. Among OPMDs, leukoplakia showed significant increase in nuclear area, nuclear perimeter, Nuclear/Cytoplasm (N/C) ratio, density and loss of polarity, while OSF showed significance with only perimeter, density and loss of polarity. CONCLUSION: Based on findings of present study, it is concluded that measurements using computer- aided morphometric analysis may provide an objective means for predicting the malignant transformation OPMDs to OSCC. Among OPMDs, Leukoplakia has a higher chance of malignant transformation than OSF