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Immunohistochemical analysis of Nuclear Factor-kappa B (NF-κB) between follicular and plexiform ameloblastomas: A pilot study
BACKGROUND: Ameloblastoma among benign tumors holds a unique position by its locally destructive and invasive nature. Tumors that originate from the odontogenic apparatus or its remnants in the jaws show diverse clinical presentations, behavior and histologic patterns. The differed biological behavi...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8083443/ https://www.ncbi.nlm.nih.gov/pubmed/33967482 http://dx.doi.org/10.4103/jomfp.JOMFP_150_20 |
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author | Sivakumar, Muniapillai Yoithapprabhunath, Thuckanaickenpalayam Ragunathan Nirmal, Ramadas Madhavan Veeravarmal, Veeran Dineshshankar, Janardhanam Amsaveni, Ramamoorthy |
author_facet | Sivakumar, Muniapillai Yoithapprabhunath, Thuckanaickenpalayam Ragunathan Nirmal, Ramadas Madhavan Veeravarmal, Veeran Dineshshankar, Janardhanam Amsaveni, Ramamoorthy |
author_sort | Sivakumar, Muniapillai |
collection | PubMed |
description | BACKGROUND: Ameloblastoma among benign tumors holds a unique position by its locally destructive and invasive nature. Tumors that originate from the odontogenic apparatus or its remnants in the jaws show diverse clinical presentations, behavior and histologic patterns. The differed biological behavior behind follicular and plexiform ameloblastomas has never attained completeness because of the lack of rhythmic correlation regarding the exact mechanism. Nuclear factor-kappa B (NF-κB) pathways play a crucial role in survival, death and differentiation during physiologic and pathologic conditions. With this background, the study has been aimed to investigate the expression of NF-κB in follicular and plexiform ameloblastomas. OBJECTIVE: The objective of this study was to analyze the immunohistochemical expression pattern of NF-κB in ameloblastoma and to compare the immunohistochemical expression pattern of NF-κB among the histological types of ameloblastoma, follicular and plexiform patterns. METHODOLOGY: Total 20 ameloblastomas (10 follicular, 10 plexiform) were immunostained with antihuman NF-κB p65 mouse IgG monoclonal antibody, and the pattern of staining is statistically analyzed using Chi-square test with the level of significance (P < 0.05). RESULTS: Twelve (3 follicular, 9 plexiform) out of 20 ameloblastomas showed immunoreactivity to NF-κB p65. In ameloblastoma, only the peripheral preameloblast-like tall columnar cells showed reactivity, whereas the stellate reticulum-like cells are immunonegative. The staining pattern was membranous in the immunoreactive cells. The results were studied with the associated and inducing pathways from the literature, and a possible mechanism has been proposed. CONCLUSION: The expression pattern of NF-κB was found to be higher in plexiform ameloblastoma than follicular ameloblastoma. |
format | Online Article Text |
id | pubmed-8083443 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-80834432021-05-06 Immunohistochemical analysis of Nuclear Factor-kappa B (NF-κB) between follicular and plexiform ameloblastomas: A pilot study Sivakumar, Muniapillai Yoithapprabhunath, Thuckanaickenpalayam Ragunathan Nirmal, Ramadas Madhavan Veeravarmal, Veeran Dineshshankar, Janardhanam Amsaveni, Ramamoorthy J Oral Maxillofac Pathol Original Article BACKGROUND: Ameloblastoma among benign tumors holds a unique position by its locally destructive and invasive nature. Tumors that originate from the odontogenic apparatus or its remnants in the jaws show diverse clinical presentations, behavior and histologic patterns. The differed biological behavior behind follicular and plexiform ameloblastomas has never attained completeness because of the lack of rhythmic correlation regarding the exact mechanism. Nuclear factor-kappa B (NF-κB) pathways play a crucial role in survival, death and differentiation during physiologic and pathologic conditions. With this background, the study has been aimed to investigate the expression of NF-κB in follicular and plexiform ameloblastomas. OBJECTIVE: The objective of this study was to analyze the immunohistochemical expression pattern of NF-κB in ameloblastoma and to compare the immunohistochemical expression pattern of NF-κB among the histological types of ameloblastoma, follicular and plexiform patterns. METHODOLOGY: Total 20 ameloblastomas (10 follicular, 10 plexiform) were immunostained with antihuman NF-κB p65 mouse IgG monoclonal antibody, and the pattern of staining is statistically analyzed using Chi-square test with the level of significance (P < 0.05). RESULTS: Twelve (3 follicular, 9 plexiform) out of 20 ameloblastomas showed immunoreactivity to NF-κB p65. In ameloblastoma, only the peripheral preameloblast-like tall columnar cells showed reactivity, whereas the stellate reticulum-like cells are immunonegative. The staining pattern was membranous in the immunoreactive cells. The results were studied with the associated and inducing pathways from the literature, and a possible mechanism has been proposed. CONCLUSION: The expression pattern of NF-κB was found to be higher in plexiform ameloblastoma than follicular ameloblastoma. Wolters Kluwer - Medknow 2020 2021-01-09 /pmc/articles/PMC8083443/ /pubmed/33967482 http://dx.doi.org/10.4103/jomfp.JOMFP_150_20 Text en Copyright: © 2021 Journal of Oral and Maxillofacial Pathology https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Sivakumar, Muniapillai Yoithapprabhunath, Thuckanaickenpalayam Ragunathan Nirmal, Ramadas Madhavan Veeravarmal, Veeran Dineshshankar, Janardhanam Amsaveni, Ramamoorthy Immunohistochemical analysis of Nuclear Factor-kappa B (NF-κB) between follicular and plexiform ameloblastomas: A pilot study |
title | Immunohistochemical analysis of Nuclear Factor-kappa B (NF-κB) between follicular and plexiform ameloblastomas: A pilot study |
title_full | Immunohistochemical analysis of Nuclear Factor-kappa B (NF-κB) between follicular and plexiform ameloblastomas: A pilot study |
title_fullStr | Immunohistochemical analysis of Nuclear Factor-kappa B (NF-κB) between follicular and plexiform ameloblastomas: A pilot study |
title_full_unstemmed | Immunohistochemical analysis of Nuclear Factor-kappa B (NF-κB) between follicular and plexiform ameloblastomas: A pilot study |
title_short | Immunohistochemical analysis of Nuclear Factor-kappa B (NF-κB) between follicular and plexiform ameloblastomas: A pilot study |
title_sort | immunohistochemical analysis of nuclear factor-kappa b (nf-κb) between follicular and plexiform ameloblastomas: a pilot study |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8083443/ https://www.ncbi.nlm.nih.gov/pubmed/33967482 http://dx.doi.org/10.4103/jomfp.JOMFP_150_20 |
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