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Biological and Genetic Characterization of Pod Pepper Vein Yellows Virus-Associated RNA From Capsicum frutescens in Wenshan, China

Tombusvirus-like associated RNAs (tlaRNAs) are positive-sense single-stranded RNAs found in plants co-infected with some viruses of the genus Polerovirus. Pod pepper vein yellows virus (PoPeVYV) was recently reported as a new recombinant polerovirus causing interveinal yellowing, stunting, and leaf...

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Autores principales: Peng, Jiejun, Bu, Shan, Yin, Yueyan, Hua, Mengying, Zhao, Kuangjie, Lu, Yuwen, Zheng, Hongying, Wan, Qionglian, Zhang, Songbai, Chen, Hairu, Liu, Yong, Chen, Jianping, Mo, Xiaohan, Yan, Fei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8083956/
https://www.ncbi.nlm.nih.gov/pubmed/33936020
http://dx.doi.org/10.3389/fmicb.2021.662352
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author Peng, Jiejun
Bu, Shan
Yin, Yueyan
Hua, Mengying
Zhao, Kuangjie
Lu, Yuwen
Zheng, Hongying
Wan, Qionglian
Zhang, Songbai
Chen, Hairu
Liu, Yong
Chen, Jianping
Mo, Xiaohan
Yan, Fei
author_facet Peng, Jiejun
Bu, Shan
Yin, Yueyan
Hua, Mengying
Zhao, Kuangjie
Lu, Yuwen
Zheng, Hongying
Wan, Qionglian
Zhang, Songbai
Chen, Hairu
Liu, Yong
Chen, Jianping
Mo, Xiaohan
Yan, Fei
author_sort Peng, Jiejun
collection PubMed
description Tombusvirus-like associated RNAs (tlaRNAs) are positive-sense single-stranded RNAs found in plants co-infected with some viruses of the genus Polerovirus. Pod pepper vein yellows virus (PoPeVYV) was recently reported as a new recombinant polerovirus causing interveinal yellowing, stunting, and leaf rolling in Capsicum frutescens plants at Wenshan city, Yunnan province, China. The complete genome sequence of its associated RNA has now been determined by next-generation sequencing and reverse transcription (RT) polymerase chain reaction (PCR). PoPeVYV-associated RNA (PoPeVYVaRNA) (GenBank Accession No. MW323470) has 2970 nucleotides and is closely related to other group II tlaRNAs, particularly tobacco bushy top disease-associated RNA (TBTDaRNA, GenBank Accession No. EF529625). In infection experiments on Nicotiana benthamiana and C. frutescens plants, synergism between PoPeVYVaRNA and PoPeVYV was demonstrated, leading to severe interveinal yellowing of leaves and stunting of plants. The results provide further information on the genetic and biological properties of the various agents associated with pepper vein yellows disease (PeVYD).
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spelling pubmed-80839562021-04-30 Biological and Genetic Characterization of Pod Pepper Vein Yellows Virus-Associated RNA From Capsicum frutescens in Wenshan, China Peng, Jiejun Bu, Shan Yin, Yueyan Hua, Mengying Zhao, Kuangjie Lu, Yuwen Zheng, Hongying Wan, Qionglian Zhang, Songbai Chen, Hairu Liu, Yong Chen, Jianping Mo, Xiaohan Yan, Fei Front Microbiol Microbiology Tombusvirus-like associated RNAs (tlaRNAs) are positive-sense single-stranded RNAs found in plants co-infected with some viruses of the genus Polerovirus. Pod pepper vein yellows virus (PoPeVYV) was recently reported as a new recombinant polerovirus causing interveinal yellowing, stunting, and leaf rolling in Capsicum frutescens plants at Wenshan city, Yunnan province, China. The complete genome sequence of its associated RNA has now been determined by next-generation sequencing and reverse transcription (RT) polymerase chain reaction (PCR). PoPeVYV-associated RNA (PoPeVYVaRNA) (GenBank Accession No. MW323470) has 2970 nucleotides and is closely related to other group II tlaRNAs, particularly tobacco bushy top disease-associated RNA (TBTDaRNA, GenBank Accession No. EF529625). In infection experiments on Nicotiana benthamiana and C. frutescens plants, synergism between PoPeVYVaRNA and PoPeVYV was demonstrated, leading to severe interveinal yellowing of leaves and stunting of plants. The results provide further information on the genetic and biological properties of the various agents associated with pepper vein yellows disease (PeVYD). Frontiers Media S.A. 2021-04-15 /pmc/articles/PMC8083956/ /pubmed/33936020 http://dx.doi.org/10.3389/fmicb.2021.662352 Text en Copyright © 2021 Peng, Bu, Yin, Hua, Zhao, Lu, Zheng, Wan, Zhang, Chen, Liu, Chen, Mo and Yan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Peng, Jiejun
Bu, Shan
Yin, Yueyan
Hua, Mengying
Zhao, Kuangjie
Lu, Yuwen
Zheng, Hongying
Wan, Qionglian
Zhang, Songbai
Chen, Hairu
Liu, Yong
Chen, Jianping
Mo, Xiaohan
Yan, Fei
Biological and Genetic Characterization of Pod Pepper Vein Yellows Virus-Associated RNA From Capsicum frutescens in Wenshan, China
title Biological and Genetic Characterization of Pod Pepper Vein Yellows Virus-Associated RNA From Capsicum frutescens in Wenshan, China
title_full Biological and Genetic Characterization of Pod Pepper Vein Yellows Virus-Associated RNA From Capsicum frutescens in Wenshan, China
title_fullStr Biological and Genetic Characterization of Pod Pepper Vein Yellows Virus-Associated RNA From Capsicum frutescens in Wenshan, China
title_full_unstemmed Biological and Genetic Characterization of Pod Pepper Vein Yellows Virus-Associated RNA From Capsicum frutescens in Wenshan, China
title_short Biological and Genetic Characterization of Pod Pepper Vein Yellows Virus-Associated RNA From Capsicum frutescens in Wenshan, China
title_sort biological and genetic characterization of pod pepper vein yellows virus-associated rna from capsicum frutescens in wenshan, china
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8083956/
https://www.ncbi.nlm.nih.gov/pubmed/33936020
http://dx.doi.org/10.3389/fmicb.2021.662352
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