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Taurine Attenuates the Hypotaurine-Induced Progression of CRC via ERK/RSK Signaling

Colorectal cancer (CRC) is one of the most common malignant tumors, and previous metabolomics work has demonstrated great promise in identifying specific small molecules of tumor phenotype. In the present study, we analyzed the metabolites of resected tissues through gas chromatography-mass spectrom...

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Autores principales: Hou, Xiaodan, Hu, Junwei, Zhao, Xinyu, Wei, Qing, Zhao, Rongping, Li, Min, Li, Qiong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8083965/
https://www.ncbi.nlm.nih.gov/pubmed/33937232
http://dx.doi.org/10.3389/fcell.2021.631163
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author Hou, Xiaodan
Hu, Junwei
Zhao, Xinyu
Wei, Qing
Zhao, Rongping
Li, Min
Li, Qiong
author_facet Hou, Xiaodan
Hu, Junwei
Zhao, Xinyu
Wei, Qing
Zhao, Rongping
Li, Min
Li, Qiong
author_sort Hou, Xiaodan
collection PubMed
description Colorectal cancer (CRC) is one of the most common malignant tumors, and previous metabolomics work has demonstrated great promise in identifying specific small molecules of tumor phenotype. In the present study, we analyzed the metabolites of resected tissues through gas chromatography-mass spectrometry (GC-MS), and found that the concentration of taurine in CRC tissues diminished whereas the concentration of hypotaurine increased. The results in vitro demonstrated that taurine significantly suppressed cellular proliferation, metastasis, and colony formation whereas it induced apoptosis in CRC cells. Furthermore, taurine regulated the expression levels of epithelial mesenchymal transition (EMT)-associated genes in a dose-dependent manner. Taurine also alleviated hypotaurine-induced CRC progression, which was linked to the inhibition of the ERK/RSK-signaling pathway and diminution in intracellular hypotaurine. Taurine additionally attenuated hypotaurine-induced tumor growth and metastasis in vivo. Patients with CRC exhibited lower levels of serum taurine, suggesting that taurine might be a promising biomarker reflecting a poor prognosis in CRC. Collectively, our results demonstrated that taurine-attenuated, hypotaurine-induced CRC progression provides a potential target for CRC therapy.
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spelling pubmed-80839652021-04-30 Taurine Attenuates the Hypotaurine-Induced Progression of CRC via ERK/RSK Signaling Hou, Xiaodan Hu, Junwei Zhao, Xinyu Wei, Qing Zhao, Rongping Li, Min Li, Qiong Front Cell Dev Biol Cell and Developmental Biology Colorectal cancer (CRC) is one of the most common malignant tumors, and previous metabolomics work has demonstrated great promise in identifying specific small molecules of tumor phenotype. In the present study, we analyzed the metabolites of resected tissues through gas chromatography-mass spectrometry (GC-MS), and found that the concentration of taurine in CRC tissues diminished whereas the concentration of hypotaurine increased. The results in vitro demonstrated that taurine significantly suppressed cellular proliferation, metastasis, and colony formation whereas it induced apoptosis in CRC cells. Furthermore, taurine regulated the expression levels of epithelial mesenchymal transition (EMT)-associated genes in a dose-dependent manner. Taurine also alleviated hypotaurine-induced CRC progression, which was linked to the inhibition of the ERK/RSK-signaling pathway and diminution in intracellular hypotaurine. Taurine additionally attenuated hypotaurine-induced tumor growth and metastasis in vivo. Patients with CRC exhibited lower levels of serum taurine, suggesting that taurine might be a promising biomarker reflecting a poor prognosis in CRC. Collectively, our results demonstrated that taurine-attenuated, hypotaurine-induced CRC progression provides a potential target for CRC therapy. Frontiers Media S.A. 2021-04-15 /pmc/articles/PMC8083965/ /pubmed/33937232 http://dx.doi.org/10.3389/fcell.2021.631163 Text en Copyright © 2021 Hou, Hu, Zhao, Wei, Zhao, Li and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Hou, Xiaodan
Hu, Junwei
Zhao, Xinyu
Wei, Qing
Zhao, Rongping
Li, Min
Li, Qiong
Taurine Attenuates the Hypotaurine-Induced Progression of CRC via ERK/RSK Signaling
title Taurine Attenuates the Hypotaurine-Induced Progression of CRC via ERK/RSK Signaling
title_full Taurine Attenuates the Hypotaurine-Induced Progression of CRC via ERK/RSK Signaling
title_fullStr Taurine Attenuates the Hypotaurine-Induced Progression of CRC via ERK/RSK Signaling
title_full_unstemmed Taurine Attenuates the Hypotaurine-Induced Progression of CRC via ERK/RSK Signaling
title_short Taurine Attenuates the Hypotaurine-Induced Progression of CRC via ERK/RSK Signaling
title_sort taurine attenuates the hypotaurine-induced progression of crc via erk/rsk signaling
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8083965/
https://www.ncbi.nlm.nih.gov/pubmed/33937232
http://dx.doi.org/10.3389/fcell.2021.631163
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