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Proteomics study of serum exosomes in Kawasaki disease patients with coronary artery aneurysms
BACKGROUND: To study the protein profile of the serum exosomes of patients with coronary artery aneurysms (CAA) caused by Kawasaki disease (KD). METHODS: Two-dimensional electrophoresis (2-DE) was used to identify proteins from the exosomes of serum obtained from children with CAA caused by KD, as w...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Via Medica
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084394/ https://www.ncbi.nlm.nih.gov/pubmed/29611167 http://dx.doi.org/10.5603/CJ.a2018.0032 |
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author | Xie, Xiao-Fei Chu, Hong-Juan Xu, Yu-Fen Hua, Liang Wang, Zhou-Ping Huang, Ping Jia, Hong-Ling Zhang, Li |
author_facet | Xie, Xiao-Fei Chu, Hong-Juan Xu, Yu-Fen Hua, Liang Wang, Zhou-Ping Huang, Ping Jia, Hong-Ling Zhang, Li |
author_sort | Xie, Xiao-Fei |
collection | PubMed |
description | BACKGROUND: To study the protein profile of the serum exosomes of patients with coronary artery aneurysms (CAA) caused by Kawasaki disease (KD). METHODS: Two-dimensional electrophoresis (2-DE) was used to identify proteins from the exosomes of serum obtained from children with CAA caused by KD, as well as healthy controls. Differentially expressed proteins were identified using matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF MS) analysis. RESULTS: Thirty two differentially expressed proteins were identified (18 up-regulated and 14 downregulated) from serum exosomes of children with CAA and were compared to healthy controls. The expression levels of 4 proteins (TN, RBP4, LRG1, and APOA4) were validated using Western blotting. Classification analysis and protein–protein network analysis showed that they are associated with multiple functional groups, including host immune response, inflammation, apoptotic process, developmental process, and biological adhesion process. CONCLUSIONS: These findings establish a comprehensive proteomic profile of serum exosomes from children with CAA caused by KD, and provide additional insights into the mechanisms of CAA caused by KD. |
format | Online Article Text |
id | pubmed-8084394 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Via Medica |
record_format | MEDLINE/PubMed |
spelling | pubmed-80843942021-05-10 Proteomics study of serum exosomes in Kawasaki disease patients with coronary artery aneurysms Xie, Xiao-Fei Chu, Hong-Juan Xu, Yu-Fen Hua, Liang Wang, Zhou-Ping Huang, Ping Jia, Hong-Ling Zhang, Li Cardiol J Basic Science and Experimental Cardiology BACKGROUND: To study the protein profile of the serum exosomes of patients with coronary artery aneurysms (CAA) caused by Kawasaki disease (KD). METHODS: Two-dimensional electrophoresis (2-DE) was used to identify proteins from the exosomes of serum obtained from children with CAA caused by KD, as well as healthy controls. Differentially expressed proteins were identified using matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF MS) analysis. RESULTS: Thirty two differentially expressed proteins were identified (18 up-regulated and 14 downregulated) from serum exosomes of children with CAA and were compared to healthy controls. The expression levels of 4 proteins (TN, RBP4, LRG1, and APOA4) were validated using Western blotting. Classification analysis and protein–protein network analysis showed that they are associated with multiple functional groups, including host immune response, inflammation, apoptotic process, developmental process, and biological adhesion process. CONCLUSIONS: These findings establish a comprehensive proteomic profile of serum exosomes from children with CAA caused by KD, and provide additional insights into the mechanisms of CAA caused by KD. Via Medica 2019-11-06 /pmc/articles/PMC8084394/ /pubmed/29611167 http://dx.doi.org/10.5603/CJ.a2018.0032 Text en Copyright © 2019 Via Medica https://creativecommons.org/licenses/by-nc-nd/4.0/This article is available in open access under Creative Common Attribution-Non-Commercial-No Derivatives 4.0 International (CC BY-NC-ND 4.0) license, allowing to download articles and share them with others as long as they credit the authors and the publisher, but without permission to change them in any way or use them commercially. |
spellingShingle | Basic Science and Experimental Cardiology Xie, Xiao-Fei Chu, Hong-Juan Xu, Yu-Fen Hua, Liang Wang, Zhou-Ping Huang, Ping Jia, Hong-Ling Zhang, Li Proteomics study of serum exosomes in Kawasaki disease patients with coronary artery aneurysms |
title | Proteomics study of serum exosomes in Kawasaki disease patients with coronary artery aneurysms |
title_full | Proteomics study of serum exosomes in Kawasaki disease patients with coronary artery aneurysms |
title_fullStr | Proteomics study of serum exosomes in Kawasaki disease patients with coronary artery aneurysms |
title_full_unstemmed | Proteomics study of serum exosomes in Kawasaki disease patients with coronary artery aneurysms |
title_short | Proteomics study of serum exosomes in Kawasaki disease patients with coronary artery aneurysms |
title_sort | proteomics study of serum exosomes in kawasaki disease patients with coronary artery aneurysms |
topic | Basic Science and Experimental Cardiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084394/ https://www.ncbi.nlm.nih.gov/pubmed/29611167 http://dx.doi.org/10.5603/CJ.a2018.0032 |
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