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Transforming a well into a chip: A modular 3D-printed microfluidic chip

Organ-on-a-Chip platforms provide rich opportunities to observe interactions between different cell types under in vivo-like conditions, i.e., in the presence of flow. Yet, the costs and know-how required for the fabrication and implementation of these platforms restrict their accessibility. This st...

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Autores principales: Rauti, Rossana, Ess, Adi, Le Roi, Baptiste, Kreinin, Yevgeniy, Epshtein, Mark, Korin, Netanel, Maoz, Ben M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AIP Publishing LLC 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084581/
https://www.ncbi.nlm.nih.gov/pubmed/33948527
http://dx.doi.org/10.1063/5.0039366
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author Rauti, Rossana
Ess, Adi
Le Roi, Baptiste
Kreinin, Yevgeniy
Epshtein, Mark
Korin, Netanel
Maoz, Ben M.
author_facet Rauti, Rossana
Ess, Adi
Le Roi, Baptiste
Kreinin, Yevgeniy
Epshtein, Mark
Korin, Netanel
Maoz, Ben M.
author_sort Rauti, Rossana
collection PubMed
description Organ-on-a-Chip platforms provide rich opportunities to observe interactions between different cell types under in vivo-like conditions, i.e., in the presence of flow. Yet, the costs and know-how required for the fabrication and implementation of these platforms restrict their accessibility. This study introduces and demonstrates a novel Insert-Chip: a microfluidic device that provides the functionality of an Organ-on-a-Chip platform, namely, the capacity to co-culture cells, expose them to flow, and observe their interactions—yet can easily be integrated into standard culture systems (e.g., well plates or multi-electrode arrays). The device is produced using stereolithograpy 3D printing and is user-friendly and reusable. Moreover, its design features overcome some of the measurement and imaging challenges characterizing standard Organ-on-a-Chip platforms. We have co-cultured endothelial and epithelial cells under flow conditions to demonstrate the functionality of the device. Overall, this novel microfluidic device is a promising platform for the investigation of biological functions, cell–cell interactions, and response to therapeutics.
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spelling pubmed-80845812021-05-03 Transforming a well into a chip: A modular 3D-printed microfluidic chip Rauti, Rossana Ess, Adi Le Roi, Baptiste Kreinin, Yevgeniy Epshtein, Mark Korin, Netanel Maoz, Ben M. APL Bioeng Articles Organ-on-a-Chip platforms provide rich opportunities to observe interactions between different cell types under in vivo-like conditions, i.e., in the presence of flow. Yet, the costs and know-how required for the fabrication and implementation of these platforms restrict their accessibility. This study introduces and demonstrates a novel Insert-Chip: a microfluidic device that provides the functionality of an Organ-on-a-Chip platform, namely, the capacity to co-culture cells, expose them to flow, and observe their interactions—yet can easily be integrated into standard culture systems (e.g., well plates or multi-electrode arrays). The device is produced using stereolithograpy 3D printing and is user-friendly and reusable. Moreover, its design features overcome some of the measurement and imaging challenges characterizing standard Organ-on-a-Chip platforms. We have co-cultured endothelial and epithelial cells under flow conditions to demonstrate the functionality of the device. Overall, this novel microfluidic device is a promising platform for the investigation of biological functions, cell–cell interactions, and response to therapeutics. AIP Publishing LLC 2021-04-28 /pmc/articles/PMC8084581/ /pubmed/33948527 http://dx.doi.org/10.1063/5.0039366 Text en © 2021 Author(s). 2473-2877/2021/5(2)/026103/13 https://creativecommons.org/licenses/by/4.0/All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Articles
Rauti, Rossana
Ess, Adi
Le Roi, Baptiste
Kreinin, Yevgeniy
Epshtein, Mark
Korin, Netanel
Maoz, Ben M.
Transforming a well into a chip: A modular 3D-printed microfluidic chip
title Transforming a well into a chip: A modular 3D-printed microfluidic chip
title_full Transforming a well into a chip: A modular 3D-printed microfluidic chip
title_fullStr Transforming a well into a chip: A modular 3D-printed microfluidic chip
title_full_unstemmed Transforming a well into a chip: A modular 3D-printed microfluidic chip
title_short Transforming a well into a chip: A modular 3D-printed microfluidic chip
title_sort transforming a well into a chip: a modular 3d-printed microfluidic chip
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084581/
https://www.ncbi.nlm.nih.gov/pubmed/33948527
http://dx.doi.org/10.1063/5.0039366
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