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Role of Shear Stress on Renal Proximal Tubular Cells for Nephrotoxicity Assays

Drug-induced nephrotoxicity causes huge morbidity and mortality at massive financial cost. The greatest burden of drug-induced acute kidney injury falls on the proximal tubular cells. To maintain their structure and function, renal proximal tubular cells need the shear stress from tubular fluid flow...

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Detalles Bibliográficos
Autores principales: Birdsall, Holly H., Hammond, Timothy G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084667/
https://www.ncbi.nlm.nih.gov/pubmed/33976696
http://dx.doi.org/10.1155/2021/6643324
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author Birdsall, Holly H.
Hammond, Timothy G.
author_facet Birdsall, Holly H.
Hammond, Timothy G.
author_sort Birdsall, Holly H.
collection PubMed
description Drug-induced nephrotoxicity causes huge morbidity and mortality at massive financial cost. The greatest burden of drug-induced acute kidney injury falls on the proximal tubular cells. To maintain their structure and function, renal proximal tubular cells need the shear stress from tubular fluid flow. Diverse techniques to reintroduce shear stress have been studied in a variety of proximal tubular like cell culture models. These studies often have limited replicates because of the huge cost of equipment and do not report all relevant parameters to allow reproduction and comparison of studies between labs. This review codifies the techniques used to reintroduce shear stress, the cell lines utilized, and the biological outcomes reported. Further, we propose a set of interventions to enhance future cell biology understanding of nephrotoxicity using cell culture models.
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spelling pubmed-80846672021-05-10 Role of Shear Stress on Renal Proximal Tubular Cells for Nephrotoxicity Assays Birdsall, Holly H. Hammond, Timothy G. J Toxicol Review Article Drug-induced nephrotoxicity causes huge morbidity and mortality at massive financial cost. The greatest burden of drug-induced acute kidney injury falls on the proximal tubular cells. To maintain their structure and function, renal proximal tubular cells need the shear stress from tubular fluid flow. Diverse techniques to reintroduce shear stress have been studied in a variety of proximal tubular like cell culture models. These studies often have limited replicates because of the huge cost of equipment and do not report all relevant parameters to allow reproduction and comparison of studies between labs. This review codifies the techniques used to reintroduce shear stress, the cell lines utilized, and the biological outcomes reported. Further, we propose a set of interventions to enhance future cell biology understanding of nephrotoxicity using cell culture models. Hindawi 2021-04-21 /pmc/articles/PMC8084667/ /pubmed/33976696 http://dx.doi.org/10.1155/2021/6643324 Text en Copyright © 2021 Holly H. Birdsall and Timothy G. Hammond. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Birdsall, Holly H.
Hammond, Timothy G.
Role of Shear Stress on Renal Proximal Tubular Cells for Nephrotoxicity Assays
title Role of Shear Stress on Renal Proximal Tubular Cells for Nephrotoxicity Assays
title_full Role of Shear Stress on Renal Proximal Tubular Cells for Nephrotoxicity Assays
title_fullStr Role of Shear Stress on Renal Proximal Tubular Cells for Nephrotoxicity Assays
title_full_unstemmed Role of Shear Stress on Renal Proximal Tubular Cells for Nephrotoxicity Assays
title_short Role of Shear Stress on Renal Proximal Tubular Cells for Nephrotoxicity Assays
title_sort role of shear stress on renal proximal tubular cells for nephrotoxicity assays
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084667/
https://www.ncbi.nlm.nih.gov/pubmed/33976696
http://dx.doi.org/10.1155/2021/6643324
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