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Anti-SASP and anti-inflammatory activity of resveratrol, curcumin and β-caryophyllene association on human endothelial and monocytic cells

A challenging and promising new branch of aging-related research fields is the identification of natural compounds able to modulate the senescence-associated secretory phenotype (SASP), which characterizes senescent cells and can contribute to fuel the inflammaging. We investigated both the anti-SAS...

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Autores principales: Matacchione, Giulia, Gurău, Felicia, Silvestrini, Andrea, Tiboni, Mattia, Mancini, Luca, Valli, Debora, Rippo, Maria Rita, Recchioni, Rina, Marcheselli, Fiorella, Carnevali, Oliana, Procopio, Antonio Domenico, Casettari, Luca, Olivieri, Fabiola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084815/
https://www.ncbi.nlm.nih.gov/pubmed/33704623
http://dx.doi.org/10.1007/s10522-021-09915-0
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author Matacchione, Giulia
Gurău, Felicia
Silvestrini, Andrea
Tiboni, Mattia
Mancini, Luca
Valli, Debora
Rippo, Maria Rita
Recchioni, Rina
Marcheselli, Fiorella
Carnevali, Oliana
Procopio, Antonio Domenico
Casettari, Luca
Olivieri, Fabiola
author_facet Matacchione, Giulia
Gurău, Felicia
Silvestrini, Andrea
Tiboni, Mattia
Mancini, Luca
Valli, Debora
Rippo, Maria Rita
Recchioni, Rina
Marcheselli, Fiorella
Carnevali, Oliana
Procopio, Antonio Domenico
Casettari, Luca
Olivieri, Fabiola
author_sort Matacchione, Giulia
collection PubMed
description A challenging and promising new branch of aging-related research fields is the identification of natural compounds able to modulate the senescence-associated secretory phenotype (SASP), which characterizes senescent cells and can contribute to fuel the inflammaging. We investigated both the anti-SASP and anti-inflammatory activities of a nutritional supplement, namely Fenoxidol™, composed of turmeric extract bioCurcumin (bCUR), Polydatin (the natural glycosylated precursor of Resveratrol-RSV), and liposomal β-caryophyllene (BCP), in two human cellular models, such as the primary endothelial cell line, HUVECs and the monocytic cell line, THP-1. Replicative and Doxorubicin-induced senescent HUVECs, both chosen as cellular models of SASP, and lipopolysaccharides (LPS)-stimulated THP-1, selected as a model of the inflammatory response, were treated with the three single natural compounds or with a combination of them (MIX). In both senescent HUVEC models, MIX treatment significantly reduced IL-1β and IL-6 expression levels and p16(ink4a) protein, and also increased SIRT1 protein level, as well as downregulated miR-146a and miR-21 expression, two of the so-called inflamma-miRNAs, more effectively than the single compounds. In THP-1 cells stimulated with LPS, the MIX showed a significant effect in decreasing IL-1β, IL-6, TNF-α, and miR-146a expression levels and Caspase-1 activation, in association with an up-regulation of SIRT1 protein, compared to the single compounds. Overall, our results suggest that the three analysed compounds can have a combined effect in restraining SASP in senescent HUVECs as well as the inflammatory response in LPS-stimulated THP-1 cells. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10522-021-09915-0.
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spelling pubmed-80848152021-05-05 Anti-SASP and anti-inflammatory activity of resveratrol, curcumin and β-caryophyllene association on human endothelial and monocytic cells Matacchione, Giulia Gurău, Felicia Silvestrini, Andrea Tiboni, Mattia Mancini, Luca Valli, Debora Rippo, Maria Rita Recchioni, Rina Marcheselli, Fiorella Carnevali, Oliana Procopio, Antonio Domenico Casettari, Luca Olivieri, Fabiola Biogerontology Research Article A challenging and promising new branch of aging-related research fields is the identification of natural compounds able to modulate the senescence-associated secretory phenotype (SASP), which characterizes senescent cells and can contribute to fuel the inflammaging. We investigated both the anti-SASP and anti-inflammatory activities of a nutritional supplement, namely Fenoxidol™, composed of turmeric extract bioCurcumin (bCUR), Polydatin (the natural glycosylated precursor of Resveratrol-RSV), and liposomal β-caryophyllene (BCP), in two human cellular models, such as the primary endothelial cell line, HUVECs and the monocytic cell line, THP-1. Replicative and Doxorubicin-induced senescent HUVECs, both chosen as cellular models of SASP, and lipopolysaccharides (LPS)-stimulated THP-1, selected as a model of the inflammatory response, were treated with the three single natural compounds or with a combination of them (MIX). In both senescent HUVEC models, MIX treatment significantly reduced IL-1β and IL-6 expression levels and p16(ink4a) protein, and also increased SIRT1 protein level, as well as downregulated miR-146a and miR-21 expression, two of the so-called inflamma-miRNAs, more effectively than the single compounds. In THP-1 cells stimulated with LPS, the MIX showed a significant effect in decreasing IL-1β, IL-6, TNF-α, and miR-146a expression levels and Caspase-1 activation, in association with an up-regulation of SIRT1 protein, compared to the single compounds. Overall, our results suggest that the three analysed compounds can have a combined effect in restraining SASP in senescent HUVECs as well as the inflammatory response in LPS-stimulated THP-1 cells. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10522-021-09915-0. Springer Netherlands 2021-03-11 2021 /pmc/articles/PMC8084815/ /pubmed/33704623 http://dx.doi.org/10.1007/s10522-021-09915-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Matacchione, Giulia
Gurău, Felicia
Silvestrini, Andrea
Tiboni, Mattia
Mancini, Luca
Valli, Debora
Rippo, Maria Rita
Recchioni, Rina
Marcheselli, Fiorella
Carnevali, Oliana
Procopio, Antonio Domenico
Casettari, Luca
Olivieri, Fabiola
Anti-SASP and anti-inflammatory activity of resveratrol, curcumin and β-caryophyllene association on human endothelial and monocytic cells
title Anti-SASP and anti-inflammatory activity of resveratrol, curcumin and β-caryophyllene association on human endothelial and monocytic cells
title_full Anti-SASP and anti-inflammatory activity of resveratrol, curcumin and β-caryophyllene association on human endothelial and monocytic cells
title_fullStr Anti-SASP and anti-inflammatory activity of resveratrol, curcumin and β-caryophyllene association on human endothelial and monocytic cells
title_full_unstemmed Anti-SASP and anti-inflammatory activity of resveratrol, curcumin and β-caryophyllene association on human endothelial and monocytic cells
title_short Anti-SASP and anti-inflammatory activity of resveratrol, curcumin and β-caryophyllene association on human endothelial and monocytic cells
title_sort anti-sasp and anti-inflammatory activity of resveratrol, curcumin and β-caryophyllene association on human endothelial and monocytic cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084815/
https://www.ncbi.nlm.nih.gov/pubmed/33704623
http://dx.doi.org/10.1007/s10522-021-09915-0
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