Cargando…
Surface Modified β-Tricalcium phosphate enhanced stem cell osteogenic differentiation in vitro and bone regeneration in vivo
A major number of studies have demonstrated Beta-tricalcium phosphate (β-TCP) biocompatibility, bioactivity, and osteoconductivity characteristics in bone regeneration. The aim of this research was to enhance β-TCP's biocompatibility, and evaluate its physicochemical properties by argon glow di...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084957/ https://www.ncbi.nlm.nih.gov/pubmed/33927241 http://dx.doi.org/10.1038/s41598-021-88402-5 |
_version_ | 1783686251123048448 |
---|---|
author | Choy, Cheuk Sing Lee, Wei Fang Lin, Pei Ying Wu, Yi-Fan Huang, Haw-Ming Teng, Nai-Chia Pan, Yu-Hwa Salamanca, Eisner Chang, Wei-Jen |
author_facet | Choy, Cheuk Sing Lee, Wei Fang Lin, Pei Ying Wu, Yi-Fan Huang, Haw-Ming Teng, Nai-Chia Pan, Yu-Hwa Salamanca, Eisner Chang, Wei-Jen |
author_sort | Choy, Cheuk Sing |
collection | PubMed |
description | A major number of studies have demonstrated Beta-tricalcium phosphate (β-TCP) biocompatibility, bioactivity, and osteoconductivity characteristics in bone regeneration. The aim of this research was to enhance β-TCP's biocompatibility, and evaluate its physicochemical properties by argon glow discharge plasma (GDP) plasma surface treatment without modifying its surface. Treated β-TCP was analyzed by scanning electron microscopy (SEM), energy-dispersive spectrometry, X-ray photoelectron spectroscopy (XPS), X-ray diffraction analysis, and Fourier transform infrared spectroscopy characterization. To evaluate treated β-TCP biocompatibility and osteoblastic differentiation, water-soluble tetrazolium salts-1 (WST-1), immunofluorescence, alkaline phosphatase (ALP) assay, and quantitative real-time polymerase chain reaction (QPCR) were done using human mesenchymal stem cells (hMSCs). The results indicated a slight enhancement of the β-TCP by GDP sputtering, which resulted in a higher Ca/P ratio (2.05) than the control. Furthermore, when compared with control β-TCP, we observed an improvement of WST-1 on all days (p < 0.05) as well as of ALP activity (day 7, p < 0.05), with up-regulation of ALP, osteocalcin, and Osteoprotegerin osteogenic genes in cells cultured with the treated β-TCP. XPS and SEM results indicated that treated β-TCP’s surface was not modified. In vivo, micro-computed tomography and histomorphometric analysis indicated that the β-TCP test managed to regenerate more new bone than the untreated β-TCP and control defects at 8 weeks (p < 0.05). Argon GDP treatment is a viable method for removing macro and micro particles of < 7 μm in size from β-TCP bigger particles surfaces and therefore improving its biocompatibility with slight surface roughness modification, enhancing hMSCs proliferation, osteoblastic differentiation, and stimulating more new bone formation. |
format | Online Article Text |
id | pubmed-8084957 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-80849572021-04-30 Surface Modified β-Tricalcium phosphate enhanced stem cell osteogenic differentiation in vitro and bone regeneration in vivo Choy, Cheuk Sing Lee, Wei Fang Lin, Pei Ying Wu, Yi-Fan Huang, Haw-Ming Teng, Nai-Chia Pan, Yu-Hwa Salamanca, Eisner Chang, Wei-Jen Sci Rep Article A major number of studies have demonstrated Beta-tricalcium phosphate (β-TCP) biocompatibility, bioactivity, and osteoconductivity characteristics in bone regeneration. The aim of this research was to enhance β-TCP's biocompatibility, and evaluate its physicochemical properties by argon glow discharge plasma (GDP) plasma surface treatment without modifying its surface. Treated β-TCP was analyzed by scanning electron microscopy (SEM), energy-dispersive spectrometry, X-ray photoelectron spectroscopy (XPS), X-ray diffraction analysis, and Fourier transform infrared spectroscopy characterization. To evaluate treated β-TCP biocompatibility and osteoblastic differentiation, water-soluble tetrazolium salts-1 (WST-1), immunofluorescence, alkaline phosphatase (ALP) assay, and quantitative real-time polymerase chain reaction (QPCR) were done using human mesenchymal stem cells (hMSCs). The results indicated a slight enhancement of the β-TCP by GDP sputtering, which resulted in a higher Ca/P ratio (2.05) than the control. Furthermore, when compared with control β-TCP, we observed an improvement of WST-1 on all days (p < 0.05) as well as of ALP activity (day 7, p < 0.05), with up-regulation of ALP, osteocalcin, and Osteoprotegerin osteogenic genes in cells cultured with the treated β-TCP. XPS and SEM results indicated that treated β-TCP’s surface was not modified. In vivo, micro-computed tomography and histomorphometric analysis indicated that the β-TCP test managed to regenerate more new bone than the untreated β-TCP and control defects at 8 weeks (p < 0.05). Argon GDP treatment is a viable method for removing macro and micro particles of < 7 μm in size from β-TCP bigger particles surfaces and therefore improving its biocompatibility with slight surface roughness modification, enhancing hMSCs proliferation, osteoblastic differentiation, and stimulating more new bone formation. Nature Publishing Group UK 2021-04-29 /pmc/articles/PMC8084957/ /pubmed/33927241 http://dx.doi.org/10.1038/s41598-021-88402-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Choy, Cheuk Sing Lee, Wei Fang Lin, Pei Ying Wu, Yi-Fan Huang, Haw-Ming Teng, Nai-Chia Pan, Yu-Hwa Salamanca, Eisner Chang, Wei-Jen Surface Modified β-Tricalcium phosphate enhanced stem cell osteogenic differentiation in vitro and bone regeneration in vivo |
title | Surface Modified β-Tricalcium phosphate enhanced stem cell osteogenic differentiation in vitro and bone regeneration in vivo |
title_full | Surface Modified β-Tricalcium phosphate enhanced stem cell osteogenic differentiation in vitro and bone regeneration in vivo |
title_fullStr | Surface Modified β-Tricalcium phosphate enhanced stem cell osteogenic differentiation in vitro and bone regeneration in vivo |
title_full_unstemmed | Surface Modified β-Tricalcium phosphate enhanced stem cell osteogenic differentiation in vitro and bone regeneration in vivo |
title_short | Surface Modified β-Tricalcium phosphate enhanced stem cell osteogenic differentiation in vitro and bone regeneration in vivo |
title_sort | surface modified β-tricalcium phosphate enhanced stem cell osteogenic differentiation in vitro and bone regeneration in vivo |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084957/ https://www.ncbi.nlm.nih.gov/pubmed/33927241 http://dx.doi.org/10.1038/s41598-021-88402-5 |
work_keys_str_mv | AT choycheuksing surfacemodifiedbtricalciumphosphateenhancedstemcellosteogenicdifferentiationinvitroandboneregenerationinvivo AT leeweifang surfacemodifiedbtricalciumphosphateenhancedstemcellosteogenicdifferentiationinvitroandboneregenerationinvivo AT linpeiying surfacemodifiedbtricalciumphosphateenhancedstemcellosteogenicdifferentiationinvitroandboneregenerationinvivo AT wuyifan surfacemodifiedbtricalciumphosphateenhancedstemcellosteogenicdifferentiationinvitroandboneregenerationinvivo AT huanghawming surfacemodifiedbtricalciumphosphateenhancedstemcellosteogenicdifferentiationinvitroandboneregenerationinvivo AT tengnaichia surfacemodifiedbtricalciumphosphateenhancedstemcellosteogenicdifferentiationinvitroandboneregenerationinvivo AT panyuhwa surfacemodifiedbtricalciumphosphateenhancedstemcellosteogenicdifferentiationinvitroandboneregenerationinvivo AT salamancaeisner surfacemodifiedbtricalciumphosphateenhancedstemcellosteogenicdifferentiationinvitroandboneregenerationinvivo AT changweijen surfacemodifiedbtricalciumphosphateenhancedstemcellosteogenicdifferentiationinvitroandboneregenerationinvivo |