Cargando…
HIV tropism switch in archived DNA of HIV-HCV subjects successfully treated with direct-acting antivirals for HCV infection
We described short-term HIV tropism changes occurring in peripheral blood mononuclear cells and the correlations with HIV DNA value in HIV-HCV co-infected patients cured for HCV disease and with undetectable HIV viremia or residual viremia (RV). Plasma HIV RNA, cellular HIV DNA and tropism were eval...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085114/ https://www.ncbi.nlm.nih.gov/pubmed/33927306 http://dx.doi.org/10.1038/s41598-021-88811-6 |
_version_ | 1783686271044943872 |
---|---|
author | Basso, Monica Zago, Daniela Scaggiante, Renzo Cavinato, Silvia Pozzetto, Irene Stagni, Camilla Parisatto, Beatrice Cattelan, Anna Maria Battagin, Giuliana Sarmati, Loredana Parisi, Saverio Giuseppe |
author_facet | Basso, Monica Zago, Daniela Scaggiante, Renzo Cavinato, Silvia Pozzetto, Irene Stagni, Camilla Parisatto, Beatrice Cattelan, Anna Maria Battagin, Giuliana Sarmati, Loredana Parisi, Saverio Giuseppe |
author_sort | Basso, Monica |
collection | PubMed |
description | We described short-term HIV tropism changes occurring in peripheral blood mononuclear cells and the correlations with HIV DNA value in HIV-HCV co-infected patients cured for HCV disease and with undetectable HIV viremia or residual viremia (RV). Plasma HIV RNA, cellular HIV DNA and tropism were evaluated pre-HCV treatment (baseline, BL) and at 12(T1) and 24(T2) weeks after HCV treatment start. V3 sequences were interpreted using Geno2pheno and classified as R5 only if all three sequences had an FPR ≥ 10% and as X4 when at least one replicate sequence had an FPR < 10%. Forty-nine patients (21 with X4 and 28 with R5 virus) were enrolled. Five X4 patients and 9 R5 subjects experienced at least one tropism change,11 with RV:1/5 patients with X4 infection at BL switched at T1 versus 8/9 in the R5 group (p = 0.022977) and the difference was confirmed in subjects with RV (p = 0.02);6/9 R5 patients switching at T1 confirmed the tropism change at T2. No significant differences in HIV DNA values between patients with RV starting with a R5 or X4 tropism and experienced tropism switch or not were found. Short-term tropism switch involved almost a third of patients, in all but three cases with HIV RV. Being R5 at BL is associated to a higher instability, expressed as number of tropism changes and confirmed switch at T2. |
format | Online Article Text |
id | pubmed-8085114 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-80851142021-05-03 HIV tropism switch in archived DNA of HIV-HCV subjects successfully treated with direct-acting antivirals for HCV infection Basso, Monica Zago, Daniela Scaggiante, Renzo Cavinato, Silvia Pozzetto, Irene Stagni, Camilla Parisatto, Beatrice Cattelan, Anna Maria Battagin, Giuliana Sarmati, Loredana Parisi, Saverio Giuseppe Sci Rep Article We described short-term HIV tropism changes occurring in peripheral blood mononuclear cells and the correlations with HIV DNA value in HIV-HCV co-infected patients cured for HCV disease and with undetectable HIV viremia or residual viremia (RV). Plasma HIV RNA, cellular HIV DNA and tropism were evaluated pre-HCV treatment (baseline, BL) and at 12(T1) and 24(T2) weeks after HCV treatment start. V3 sequences were interpreted using Geno2pheno and classified as R5 only if all three sequences had an FPR ≥ 10% and as X4 when at least one replicate sequence had an FPR < 10%. Forty-nine patients (21 with X4 and 28 with R5 virus) were enrolled. Five X4 patients and 9 R5 subjects experienced at least one tropism change,11 with RV:1/5 patients with X4 infection at BL switched at T1 versus 8/9 in the R5 group (p = 0.022977) and the difference was confirmed in subjects with RV (p = 0.02);6/9 R5 patients switching at T1 confirmed the tropism change at T2. No significant differences in HIV DNA values between patients with RV starting with a R5 or X4 tropism and experienced tropism switch or not were found. Short-term tropism switch involved almost a third of patients, in all but three cases with HIV RV. Being R5 at BL is associated to a higher instability, expressed as number of tropism changes and confirmed switch at T2. Nature Publishing Group UK 2021-04-29 /pmc/articles/PMC8085114/ /pubmed/33927306 http://dx.doi.org/10.1038/s41598-021-88811-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Basso, Monica Zago, Daniela Scaggiante, Renzo Cavinato, Silvia Pozzetto, Irene Stagni, Camilla Parisatto, Beatrice Cattelan, Anna Maria Battagin, Giuliana Sarmati, Loredana Parisi, Saverio Giuseppe HIV tropism switch in archived DNA of HIV-HCV subjects successfully treated with direct-acting antivirals for HCV infection |
title | HIV tropism switch in archived DNA of HIV-HCV subjects successfully treated with direct-acting antivirals for HCV infection |
title_full | HIV tropism switch in archived DNA of HIV-HCV subjects successfully treated with direct-acting antivirals for HCV infection |
title_fullStr | HIV tropism switch in archived DNA of HIV-HCV subjects successfully treated with direct-acting antivirals for HCV infection |
title_full_unstemmed | HIV tropism switch in archived DNA of HIV-HCV subjects successfully treated with direct-acting antivirals for HCV infection |
title_short | HIV tropism switch in archived DNA of HIV-HCV subjects successfully treated with direct-acting antivirals for HCV infection |
title_sort | hiv tropism switch in archived dna of hiv-hcv subjects successfully treated with direct-acting antivirals for hcv infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085114/ https://www.ncbi.nlm.nih.gov/pubmed/33927306 http://dx.doi.org/10.1038/s41598-021-88811-6 |
work_keys_str_mv | AT bassomonica hivtropismswitchinarchiveddnaofhivhcvsubjectssuccessfullytreatedwithdirectactingantiviralsforhcvinfection AT zagodaniela hivtropismswitchinarchiveddnaofhivhcvsubjectssuccessfullytreatedwithdirectactingantiviralsforhcvinfection AT scaggianterenzo hivtropismswitchinarchiveddnaofhivhcvsubjectssuccessfullytreatedwithdirectactingantiviralsforhcvinfection AT cavinatosilvia hivtropismswitchinarchiveddnaofhivhcvsubjectssuccessfullytreatedwithdirectactingantiviralsforhcvinfection AT pozzettoirene hivtropismswitchinarchiveddnaofhivhcvsubjectssuccessfullytreatedwithdirectactingantiviralsforhcvinfection AT stagnicamilla hivtropismswitchinarchiveddnaofhivhcvsubjectssuccessfullytreatedwithdirectactingantiviralsforhcvinfection AT parisattobeatrice hivtropismswitchinarchiveddnaofhivhcvsubjectssuccessfullytreatedwithdirectactingantiviralsforhcvinfection AT cattelanannamaria hivtropismswitchinarchiveddnaofhivhcvsubjectssuccessfullytreatedwithdirectactingantiviralsforhcvinfection AT battagingiuliana hivtropismswitchinarchiveddnaofhivhcvsubjectssuccessfullytreatedwithdirectactingantiviralsforhcvinfection AT sarmatiloredana hivtropismswitchinarchiveddnaofhivhcvsubjectssuccessfullytreatedwithdirectactingantiviralsforhcvinfection AT parisisaveriogiuseppe hivtropismswitchinarchiveddnaofhivhcvsubjectssuccessfullytreatedwithdirectactingantiviralsforhcvinfection |