Cargando…
Crithidia mellificae infection in different mammalian species in Brazil
Crithidia mellificae, a monoxenous trypanosomatid considered restricted to insects, was recently reported to infect a bat. Herein, C. mellificae has been demonstrated to have a wider range of vertebrate hosts and distribution in Brazilian biomes than once thought. Parasites isolated from haemocultur...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085711/ https://www.ncbi.nlm.nih.gov/pubmed/33981571 http://dx.doi.org/10.1016/j.ijppaw.2021.04.003 |
_version_ | 1783686401947074560 |
---|---|
author | Dario, Maria Augusta Lisboa, Cristiane Varella Silva, Marlon Vicente Herrera, Heitor Miraglia Rocha, Fabiana Lopes Furtado, Marina Carvalho Moratelli, Ricardo Rodrigues Roque, André Luiz Jansen, Ana Maria |
author_facet | Dario, Maria Augusta Lisboa, Cristiane Varella Silva, Marlon Vicente Herrera, Heitor Miraglia Rocha, Fabiana Lopes Furtado, Marina Carvalho Moratelli, Ricardo Rodrigues Roque, André Luiz Jansen, Ana Maria |
author_sort | Dario, Maria Augusta |
collection | PubMed |
description | Crithidia mellificae, a monoxenous trypanosomatid considered restricted to insects, was recently reported to infect a bat. Herein, C. mellificae has been demonstrated to have a wider range of vertebrate hosts and distribution in Brazilian biomes than once thought. Parasites isolated from haemocultures were characterized using V7V8 SSU rDNA and glyceraldehyde 3-phosphate dehydrogenase genes. Coatis (Nasua nasua) in the Cerrado; marmosets (Callithrix sp.) and bats (Carollia perspicillata, Myotis lavali, M. izecksohni, Artibeus lituratus) in the Atlantic Forest; crab-eating foxes (Cerdocyon thous) and ocelot (Leopardus pardalis) in the Pantanal biomes were infected by trypanosomatids that displayed choanomastigote forms in haemoculture in Giemsa-stained slide smears. Molecular characterization and phylogenetic inference confirmed the infection of C. mellificae in these animals. Moreover, slight differences in C. mellificae sequences were observed. Crithidia mellificae growth curves were counted at 27°C, 36°C and 37°C, and the morphotypes were able to grow and survive for up to 16 days. Serological titers for C. mellificae were observed in nonhuman primates, demonstrating that this parasite is able to induce a humoral immune response in an infected mammal. These results showed that host specificity in trypanosomatids is complex and far from understood. |
format | Online Article Text |
id | pubmed-8085711 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-80857112021-05-11 Crithidia mellificae infection in different mammalian species in Brazil Dario, Maria Augusta Lisboa, Cristiane Varella Silva, Marlon Vicente Herrera, Heitor Miraglia Rocha, Fabiana Lopes Furtado, Marina Carvalho Moratelli, Ricardo Rodrigues Roque, André Luiz Jansen, Ana Maria Int J Parasitol Parasites Wildl Regular Article Crithidia mellificae, a monoxenous trypanosomatid considered restricted to insects, was recently reported to infect a bat. Herein, C. mellificae has been demonstrated to have a wider range of vertebrate hosts and distribution in Brazilian biomes than once thought. Parasites isolated from haemocultures were characterized using V7V8 SSU rDNA and glyceraldehyde 3-phosphate dehydrogenase genes. Coatis (Nasua nasua) in the Cerrado; marmosets (Callithrix sp.) and bats (Carollia perspicillata, Myotis lavali, M. izecksohni, Artibeus lituratus) in the Atlantic Forest; crab-eating foxes (Cerdocyon thous) and ocelot (Leopardus pardalis) in the Pantanal biomes were infected by trypanosomatids that displayed choanomastigote forms in haemoculture in Giemsa-stained slide smears. Molecular characterization and phylogenetic inference confirmed the infection of C. mellificae in these animals. Moreover, slight differences in C. mellificae sequences were observed. Crithidia mellificae growth curves were counted at 27°C, 36°C and 37°C, and the morphotypes were able to grow and survive for up to 16 days. Serological titers for C. mellificae were observed in nonhuman primates, demonstrating that this parasite is able to induce a humoral immune response in an infected mammal. These results showed that host specificity in trypanosomatids is complex and far from understood. Elsevier 2021-04-20 /pmc/articles/PMC8085711/ /pubmed/33981571 http://dx.doi.org/10.1016/j.ijppaw.2021.04.003 Text en © 2021 The Authors. Published by Elsevier Ltd on behalf of Australian Society for Parasitology. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Regular Article Dario, Maria Augusta Lisboa, Cristiane Varella Silva, Marlon Vicente Herrera, Heitor Miraglia Rocha, Fabiana Lopes Furtado, Marina Carvalho Moratelli, Ricardo Rodrigues Roque, André Luiz Jansen, Ana Maria Crithidia mellificae infection in different mammalian species in Brazil |
title | Crithidia mellificae infection in different mammalian species in Brazil |
title_full | Crithidia mellificae infection in different mammalian species in Brazil |
title_fullStr | Crithidia mellificae infection in different mammalian species in Brazil |
title_full_unstemmed | Crithidia mellificae infection in different mammalian species in Brazil |
title_short | Crithidia mellificae infection in different mammalian species in Brazil |
title_sort | crithidia mellificae infection in different mammalian species in brazil |
topic | Regular Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085711/ https://www.ncbi.nlm.nih.gov/pubmed/33981571 http://dx.doi.org/10.1016/j.ijppaw.2021.04.003 |
work_keys_str_mv | AT dariomariaaugusta crithidiamellificaeinfectionindifferentmammalianspeciesinbrazil AT lisboacristianevarella crithidiamellificaeinfectionindifferentmammalianspeciesinbrazil AT silvamarlonvicente crithidiamellificaeinfectionindifferentmammalianspeciesinbrazil AT herreraheitormiraglia crithidiamellificaeinfectionindifferentmammalianspeciesinbrazil AT rochafabianalopes crithidiamellificaeinfectionindifferentmammalianspeciesinbrazil AT furtadomarinacarvalho crithidiamellificaeinfectionindifferentmammalianspeciesinbrazil AT moratelliricardo crithidiamellificaeinfectionindifferentmammalianspeciesinbrazil AT rodriguesroqueandreluiz crithidiamellificaeinfectionindifferentmammalianspeciesinbrazil AT jansenanamaria crithidiamellificaeinfectionindifferentmammalianspeciesinbrazil |