Bacterial fermentation and isotope labelling optimized for amyloidogenic proteins

We developed a cost sensitive isotope labelling procedure using a fed‐batch fermentation method and tested its efficiency producing the (15)N‐, (13)C‐ and (15)N/(13)C‐labelled variants of an amyloidogenic miniprotein (E5: EEEAVRLYIQWLKEGGPSSGRPPPS). E5 is a surface active protein, which forms amyloi...

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Detalles Bibliográficos
Autores principales: Vida, István, Fazekas, Zsolt, Gyulai, Gergő, Nagy‐Fazekas, Dóra, Pálfy, Gyula, Stráner, Pál, Kiss, Éva, Perczel, András
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085922/
https://www.ncbi.nlm.nih.gov/pubmed/33739615
http://dx.doi.org/10.1111/1751-7915.13778

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085922/
https://www.ncbi.nlm.nih.gov/pubmed/33739615
http://dx.doi.org/10.1111/1751-7915.13778

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