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Bacterial fermentation and isotope labelling optimized for amyloidogenic proteins
We developed a cost sensitive isotope labelling procedure using a fed‐batch fermentation method and tested its efficiency producing the (15)N‐, (13)C‐ and (15)N/(13)C‐labelled variants of an amyloidogenic miniprotein (E5: EEEAVRLYIQWLKEGGPSSGRPPPS). E5 is a surface active protein, which forms amyloi...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085922/ https://www.ncbi.nlm.nih.gov/pubmed/33739615 http://dx.doi.org/10.1111/1751-7915.13778 |