Cargando…

A flow-cytometry-based protocol for detection of mitochondrial ROS production under hypoxia

Hypoxia is known to stimulate mitochondrial reactive oxygen species (mROS) in cells. Here, we present a detailed protocol to detect mROS using MitoSOX staining in live cells under normoxia and hypoxia. Flow cytometry allows sensitive and reliable quantification of mROS by FlowJo software. We optimiz...

Descripción completa

Detalles Bibliográficos
Autores principales:	Yang, Yun, Zhang, Guimin, Yang, Tao, Gan, Jia, Xu, Lin, Yang, Hanshuo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086139/ https://www.ncbi.nlm.nih.gov/pubmed/33997804 http://dx.doi.org/10.1016/j.xpro.2021.100466

Ejemplares similares

Protocol for analysis of mouse neutrophil NETosis by flow cytometry
por: McGill, Cassandra J., et al.
Publicado: (2021)

Protocol for simultaneous analysis of peripheral and intratumoral lymphocyte function by flow cytometry
por: Daumoine, Susy, et al.
Publicado: (2023)

Evaluating cytokine production by flow cytometry using brefeldin A in mice
por: Kovacs, Stephen B., et al.
Publicado: (2020)

Protocol for Analyzing Cell Cycle Phases of Polyphenic Wings Using Flow Cytometry
por: Zhang, Yuwei, et al.
Publicado: (2020)

Standardized flow-cytometry-based protocol to simultaneously measure transcription factor levels
por: Manso, Bryce A., et al.
Publicado: (2021)

Flow-cytometry-based protocol to analyze respiratory chain function in mouse microglia
por: Erny, Daniel, et al.
Publicado: (2022)

Protocol for high-throughput compound screening using flow cytometry in THP-1 cells
por: Spangenberg, Stephan H., et al.
Publicado: (2021)

Flow-cytometry-based protocols for human blood/marrow immunophenotyping with minimal sample perturbation
por: Rico, Laura G., et al.
Publicado: (2021)

Multiplex Cell Fate Tracking by Flow Cytometry
por: Rodríguez-Martínez, Marta, et al.
Publicado: (2020)

Protocol to analyze chromatin-bound proteins through the cell cycle using Chromoflow flow cytometry
por: Alonso-Gil, Dácil, et al.
Publicado: (2023)

Protocol for isolating human BCAM-positive corneal progenitor cells by flow cytometry and cell sorting
por: Sasamoto, Yuzuru, et al.
Publicado: (2023)

Protocol for identification and computational analysis of human natural killer cells using flow cytometry and R
por: Kroll, Kyle, et al.
Publicado: (2023)

A flow cytometry-based assay for serological detection of anti-spike antibodies in COVID-19 patients
por: Goh, Yun Shan, et al.
Publicado: (2021)

Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry
por: Liu, Zhaoyuan, et al.
Publicado: (2020)

Purification of micronuclei from cultured cells by flow cytometry
por: Toufektchan, Eléonore, et al.
Publicado: (2021)

Analyzing trogocytosis of T lymphocytes by flow cytometry and confocal microscopy
por: Zink, Alicia, et al.
Publicado: (2023)

An optimized protocol to analyze membrane protein degradation in yeast using quantitative western blot and flow cytometry
por: Arines, Felichi Mae, et al.
Publicado: (2022)

Protocol for density gradient neutrophil isolation and flow cytometry-based characterization from human peripheral blood
por: Kuang, Yi, et al.
Publicado: (2023)

Protocol for the isolation of mouse senescence-associated CD4(+) T cells using flow cytometry and functional assays
por: Fukushima, Yuji, et al.
Publicado: (2023)

Optimized protocol for mouse footpad immune cell isolation for single-cell RNA sequencing and flow cytometry
por: Hameed, Muddassar, et al.
Publicado: (2023)

Protocol to characterize the melanoma tumor immune microenvironment in mice from single cell to flow cytometry analysis
por: Sisó, Pol, et al.
Publicado: (2023)

Protocol for isolation and spectral flow cytometry analysis of immune cells from the murine exocrine and endocrine pancreas
por: Steiger, Laura, et al.
Publicado: (2023)

A flow-cytometry-based protocol using diverse cell types for detecting autoantibodies from human plasma and serum samples
por: Wong, Andrew Kam Ho, et al.
Publicado: (2021)

Mechanical isolation of neonatal and adult mouse dura leukocytes for flow cytometry analysis
por: Niu, Chunxiao, et al.
Publicado: (2023)

Analysis of T cells in mouse lymphoid tissue and blood with flow cytometry
por: Skordos, Ioannis, et al.
Publicado: (2021)

Analysis of epicardial genes in embryonic mouse hearts with flow cytometry
por: Redpath, Andia Nicole, et al.
Publicado: (2021)

Longitudinal tracking of T cell lymphomas in mice using flow cytometry
por: Kuczynski, Elizabeth A., et al.
Publicado: (2023)

Quantification of membrane-bound cytokine receptors by calibrated flow cytometry
por: Miri, Niloufarsadat, et al.
Publicado: (2023)

Novel Applications of Lead Acetate and Flow Cytometry Methods for Detection of Sulfur-Containing Molecules
por: Anishchenko, Evgeniya, et al.
Publicado: (2019)

Intracellular cytokine detection based on flow cytometry in hemocytes from Galleria mellonella larvae: A new protocol
por: Wrońska, Anna Katarzyna, et al.
Publicado: (2022)

Protocol to identify and analyze mouse and human quiescent hematopoietic stem cells using flow cytometry combined with confocal imaging
por: Qiu, Jiajing, et al.
Publicado: (2022)

CombiFlow: Flow cytometry-based identification and characterization of genetically and functionally distinct AML subclones
por: Houtsma, Roos, et al.
Publicado: (2021)

Flow cytometry detection of surface and intracellular antigens in pancreas from a single mouse embryo
por: Nyeng, Pia, et al.
Publicado: (2021)

Nuclear RNA purification by flow cytometry to study nuclear processes in plants
por: Moro, Belén, et al.
Publicado: (2021)

Isolation of spermatogenic cells from the cynomolgus macaque testis with flow cytometry
por: Lau, Xianzhong, et al.
Publicado: (2021)

Flow cytometry analysis of the subpopulations of mouse keratinocytes and skin immune cells
por: Sakamoto, Keiko, et al.
Publicado: (2021)

Isolation of human intrahepatic leukocytes for phenotypic and functional characterization by flow cytometry
por: Kucykowicz, Stephanie, et al.
Publicado: (2022)

Glutathione Quantification in Live Cells with Real-Time Imaging and Flow Cytometry
por: Jiang, Xiqian, et al.
Publicado: (2020)

Multiparametric Flow Cytometry-Based Immunophenotyping of Mouse Liver Immune Cells
por: Vanekova, Lenka, et al.
Publicado: (2022)

Flow cytometry and immunohistochemistry of the mouse dural meninges for immunological and virological assessments
por: Roussel-Queval, Annie, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_ul62nj1q198vcv2vf405t6s6u8