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A flow-cytometry-based protocol for detection of mitochondrial ROS production under hypoxia
Hypoxia is known to stimulate mitochondrial reactive oxygen species (mROS) in cells. Here, we present a detailed protocol to detect mROS using MitoSOX staining in live cells under normoxia and hypoxia. Flow cytometry allows sensitive and reliable quantification of mROS by FlowJo software. We optimiz...
Autores principales: | Yang, Yun, Zhang, Guimin, Yang, Tao, Gan, Jia, Xu, Lin, Yang, Hanshuo |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086139/ https://www.ncbi.nlm.nih.gov/pubmed/33997804 http://dx.doi.org/10.1016/j.xpro.2021.100466 |
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