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High-throughput generation of midbrain dopaminergic neuron organoids from reporter human pluripotent stem cells

Here, we describe a high-throughput 3D differentiation protocol for deriving midbrain dopaminergic neurons from human pluripotent stem cells. The use of organoids has become prevalent in disease modeling, but there is a high demand for more homogeneous cultures. Our approach is advantageous for larg...

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Detalles Bibliográficos
Autores principales:	Sarrafha, Lily, Parfitt, Gustavo M., Reyes, Ricardo, Goldman, Camille, Coccia, Elena, Kareva, Tatyana, Ahfeldt, Tim
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086141/ https://www.ncbi.nlm.nih.gov/pubmed/33997803 http://dx.doi.org/10.1016/j.xpro.2021.100463

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