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High-resolution, depth-resolved vascular leakage measurements using contrast-enhanced, correlation-gated optical coherence tomography in mice

Vascular leakage plays a key role in vision-threatening retinal diseases such as diabetic retinopathy and age-related macular degeneration. Fluorescence angiography is the current gold standard for identification of leaky vasculature in vivo, however it lacks depth resolution, providing only 2D imag...

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Autores principales: Merkle, Conrad W., Augustin, Marco, Harper, Danielle J., Gesperger, Johanna, Lichtenegger, Antonia, Eugui, Pablo, Garhöfer, Gerhard, Glösmann, Martin, Baumann, Bernhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086440/
https://www.ncbi.nlm.nih.gov/pubmed/33996197
http://dx.doi.org/10.1364/BOE.415227
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author Merkle, Conrad W.
Augustin, Marco
Harper, Danielle J.
Gesperger, Johanna
Lichtenegger, Antonia
Eugui, Pablo
Garhöfer, Gerhard
Glösmann, Martin
Baumann, Bernhard
author_facet Merkle, Conrad W.
Augustin, Marco
Harper, Danielle J.
Gesperger, Johanna
Lichtenegger, Antonia
Eugui, Pablo
Garhöfer, Gerhard
Glösmann, Martin
Baumann, Bernhard
author_sort Merkle, Conrad W.
collection PubMed
description Vascular leakage plays a key role in vision-threatening retinal diseases such as diabetic retinopathy and age-related macular degeneration. Fluorescence angiography is the current gold standard for identification of leaky vasculature in vivo, however it lacks depth resolution, providing only 2D images that complicate precise identification and localization of pathological vessels. Optical coherence tomography (OCT) has been widely adopted for clinical ophthalmology due to its high, micron-scale resolution and rapid volumetric scanning capabilities. Nevertheless, OCT cannot currently identify leaky blood vessels. To address this need, we have developed a new method called exogenous contrast-enhanced leakage OCT (ExCEL-OCT) which identifies the diffusion of tracer particles around leaky vasculature following injection of a contrast agent. We apply this method to a mouse model of retinal neovascularization and demonstrate high-resolution 3D vascular leakage measurements in vivo for the first time.
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spelling pubmed-80864402021-05-13 High-resolution, depth-resolved vascular leakage measurements using contrast-enhanced, correlation-gated optical coherence tomography in mice Merkle, Conrad W. Augustin, Marco Harper, Danielle J. Gesperger, Johanna Lichtenegger, Antonia Eugui, Pablo Garhöfer, Gerhard Glösmann, Martin Baumann, Bernhard Biomed Opt Express Article Vascular leakage plays a key role in vision-threatening retinal diseases such as diabetic retinopathy and age-related macular degeneration. Fluorescence angiography is the current gold standard for identification of leaky vasculature in vivo, however it lacks depth resolution, providing only 2D images that complicate precise identification and localization of pathological vessels. Optical coherence tomography (OCT) has been widely adopted for clinical ophthalmology due to its high, micron-scale resolution and rapid volumetric scanning capabilities. Nevertheless, OCT cannot currently identify leaky blood vessels. To address this need, we have developed a new method called exogenous contrast-enhanced leakage OCT (ExCEL-OCT) which identifies the diffusion of tracer particles around leaky vasculature following injection of a contrast agent. We apply this method to a mouse model of retinal neovascularization and demonstrate high-resolution 3D vascular leakage measurements in vivo for the first time. Optical Society of America 2021-03-02 /pmc/articles/PMC8086440/ /pubmed/33996197 http://dx.doi.org/10.1364/BOE.415227 Text en Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. https://creativecommons.org/licenses/by/4.0/https://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Merkle, Conrad W.
Augustin, Marco
Harper, Danielle J.
Gesperger, Johanna
Lichtenegger, Antonia
Eugui, Pablo
Garhöfer, Gerhard
Glösmann, Martin
Baumann, Bernhard
High-resolution, depth-resolved vascular leakage measurements using contrast-enhanced, correlation-gated optical coherence tomography in mice
title High-resolution, depth-resolved vascular leakage measurements using contrast-enhanced, correlation-gated optical coherence tomography in mice
title_full High-resolution, depth-resolved vascular leakage measurements using contrast-enhanced, correlation-gated optical coherence tomography in mice
title_fullStr High-resolution, depth-resolved vascular leakage measurements using contrast-enhanced, correlation-gated optical coherence tomography in mice
title_full_unstemmed High-resolution, depth-resolved vascular leakage measurements using contrast-enhanced, correlation-gated optical coherence tomography in mice
title_short High-resolution, depth-resolved vascular leakage measurements using contrast-enhanced, correlation-gated optical coherence tomography in mice
title_sort high-resolution, depth-resolved vascular leakage measurements using contrast-enhanced, correlation-gated optical coherence tomography in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086440/
https://www.ncbi.nlm.nih.gov/pubmed/33996197
http://dx.doi.org/10.1364/BOE.415227
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