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Clinical integration of fast Raman spectroscopy for Mohs micrographic surgery of basal cell carcinoma

We present the first clinical integration of a prototype device based on integrated auto-fluorescence imaging and Raman spectroscopy (Fast Raman device) for intra-operative assessment of surgical margins during Mohs micrographic surgery of basal cell carcinoma (BCC). Fresh skin specimens from 112 pa...

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Autores principales: Boitor, Radu, de Wolf, Coen, Weesie, Frank, Shipp, Dustin W., Varma, Sandeep, Veitch, David, Wernham, Aaron, Koloydenko, Alexey, Puppels, Gerwin, Nijsten, Tamar, Williams, Hywel C., Caspers, Peter, Notingher, Ioan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086475/
https://www.ncbi.nlm.nih.gov/pubmed/33996213
http://dx.doi.org/10.1364/BOE.417896
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author Boitor, Radu
de Wolf, Coen
Weesie, Frank
Shipp, Dustin W.
Varma, Sandeep
Veitch, David
Wernham, Aaron
Koloydenko, Alexey
Puppels, Gerwin
Nijsten, Tamar
Williams, Hywel C.
Caspers, Peter
Notingher, Ioan
author_facet Boitor, Radu
de Wolf, Coen
Weesie, Frank
Shipp, Dustin W.
Varma, Sandeep
Veitch, David
Wernham, Aaron
Koloydenko, Alexey
Puppels, Gerwin
Nijsten, Tamar
Williams, Hywel C.
Caspers, Peter
Notingher, Ioan
author_sort Boitor, Radu
collection PubMed
description We present the first clinical integration of a prototype device based on integrated auto-fluorescence imaging and Raman spectroscopy (Fast Raman device) for intra-operative assessment of surgical margins during Mohs micrographic surgery of basal cell carcinoma (BCC). Fresh skin specimens from 112 patients were used to optimise the tissue pre-processing and the Fast Raman algorithms to enable an analysis of complete Mohs layers within 30 minutes. The optimisation allowed >95% of the resection surface area to be investigated (including the deep and epidermal margins). The Fast Raman device was then used to analyse skin layers excised from the most relevant anatomical sites (nose, temple, eyelid, cheek, forehead, eyebrow and lip) and to detect the three main types of BCC (nodular, superficial and infiltrative). These results suggest that the Fast Raman technique is a promising tool to provide an objective diagnosis “tumour clear yes/no” during Mohs surgery of BCC. This clinical integration study is a key step towards a larger scale diagnosis test accuracy study to reliably determine the sensitivity and specificity in a clinical setting.
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spelling pubmed-80864752021-05-13 Clinical integration of fast Raman spectroscopy for Mohs micrographic surgery of basal cell carcinoma Boitor, Radu de Wolf, Coen Weesie, Frank Shipp, Dustin W. Varma, Sandeep Veitch, David Wernham, Aaron Koloydenko, Alexey Puppels, Gerwin Nijsten, Tamar Williams, Hywel C. Caspers, Peter Notingher, Ioan Biomed Opt Express Article We present the first clinical integration of a prototype device based on integrated auto-fluorescence imaging and Raman spectroscopy (Fast Raman device) for intra-operative assessment of surgical margins during Mohs micrographic surgery of basal cell carcinoma (BCC). Fresh skin specimens from 112 patients were used to optimise the tissue pre-processing and the Fast Raman algorithms to enable an analysis of complete Mohs layers within 30 minutes. The optimisation allowed >95% of the resection surface area to be investigated (including the deep and epidermal margins). The Fast Raman device was then used to analyse skin layers excised from the most relevant anatomical sites (nose, temple, eyelid, cheek, forehead, eyebrow and lip) and to detect the three main types of BCC (nodular, superficial and infiltrative). These results suggest that the Fast Raman technique is a promising tool to provide an objective diagnosis “tumour clear yes/no” during Mohs surgery of BCC. This clinical integration study is a key step towards a larger scale diagnosis test accuracy study to reliably determine the sensitivity and specificity in a clinical setting. Optical Society of America 2021-03-11 /pmc/articles/PMC8086475/ /pubmed/33996213 http://dx.doi.org/10.1364/BOE.417896 Text en Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. https://creativecommons.org/licenses/by/4.0/https://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Boitor, Radu
de Wolf, Coen
Weesie, Frank
Shipp, Dustin W.
Varma, Sandeep
Veitch, David
Wernham, Aaron
Koloydenko, Alexey
Puppels, Gerwin
Nijsten, Tamar
Williams, Hywel C.
Caspers, Peter
Notingher, Ioan
Clinical integration of fast Raman spectroscopy for Mohs micrographic surgery of basal cell carcinoma
title Clinical integration of fast Raman spectroscopy for Mohs micrographic surgery of basal cell carcinoma
title_full Clinical integration of fast Raman spectroscopy for Mohs micrographic surgery of basal cell carcinoma
title_fullStr Clinical integration of fast Raman spectroscopy for Mohs micrographic surgery of basal cell carcinoma
title_full_unstemmed Clinical integration of fast Raman spectroscopy for Mohs micrographic surgery of basal cell carcinoma
title_short Clinical integration of fast Raman spectroscopy for Mohs micrographic surgery of basal cell carcinoma
title_sort clinical integration of fast raman spectroscopy for mohs micrographic surgery of basal cell carcinoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086475/
https://www.ncbi.nlm.nih.gov/pubmed/33996213
http://dx.doi.org/10.1364/BOE.417896
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