Cargando…
Clinical integration of fast Raman spectroscopy for Mohs micrographic surgery of basal cell carcinoma
We present the first clinical integration of a prototype device based on integrated auto-fluorescence imaging and Raman spectroscopy (Fast Raman device) for intra-operative assessment of surgical margins during Mohs micrographic surgery of basal cell carcinoma (BCC). Fresh skin specimens from 112 pa...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Optical Society of America
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086475/ https://www.ncbi.nlm.nih.gov/pubmed/33996213 http://dx.doi.org/10.1364/BOE.417896 |
_version_ | 1783686521269780480 |
---|---|
author | Boitor, Radu de Wolf, Coen Weesie, Frank Shipp, Dustin W. Varma, Sandeep Veitch, David Wernham, Aaron Koloydenko, Alexey Puppels, Gerwin Nijsten, Tamar Williams, Hywel C. Caspers, Peter Notingher, Ioan |
author_facet | Boitor, Radu de Wolf, Coen Weesie, Frank Shipp, Dustin W. Varma, Sandeep Veitch, David Wernham, Aaron Koloydenko, Alexey Puppels, Gerwin Nijsten, Tamar Williams, Hywel C. Caspers, Peter Notingher, Ioan |
author_sort | Boitor, Radu |
collection | PubMed |
description | We present the first clinical integration of a prototype device based on integrated auto-fluorescence imaging and Raman spectroscopy (Fast Raman device) for intra-operative assessment of surgical margins during Mohs micrographic surgery of basal cell carcinoma (BCC). Fresh skin specimens from 112 patients were used to optimise the tissue pre-processing and the Fast Raman algorithms to enable an analysis of complete Mohs layers within 30 minutes. The optimisation allowed >95% of the resection surface area to be investigated (including the deep and epidermal margins). The Fast Raman device was then used to analyse skin layers excised from the most relevant anatomical sites (nose, temple, eyelid, cheek, forehead, eyebrow and lip) and to detect the three main types of BCC (nodular, superficial and infiltrative). These results suggest that the Fast Raman technique is a promising tool to provide an objective diagnosis “tumour clear yes/no” during Mohs surgery of BCC. This clinical integration study is a key step towards a larger scale diagnosis test accuracy study to reliably determine the sensitivity and specificity in a clinical setting. |
format | Online Article Text |
id | pubmed-8086475 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Optical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-80864752021-05-13 Clinical integration of fast Raman spectroscopy for Mohs micrographic surgery of basal cell carcinoma Boitor, Radu de Wolf, Coen Weesie, Frank Shipp, Dustin W. Varma, Sandeep Veitch, David Wernham, Aaron Koloydenko, Alexey Puppels, Gerwin Nijsten, Tamar Williams, Hywel C. Caspers, Peter Notingher, Ioan Biomed Opt Express Article We present the first clinical integration of a prototype device based on integrated auto-fluorescence imaging and Raman spectroscopy (Fast Raman device) for intra-operative assessment of surgical margins during Mohs micrographic surgery of basal cell carcinoma (BCC). Fresh skin specimens from 112 patients were used to optimise the tissue pre-processing and the Fast Raman algorithms to enable an analysis of complete Mohs layers within 30 minutes. The optimisation allowed >95% of the resection surface area to be investigated (including the deep and epidermal margins). The Fast Raman device was then used to analyse skin layers excised from the most relevant anatomical sites (nose, temple, eyelid, cheek, forehead, eyebrow and lip) and to detect the three main types of BCC (nodular, superficial and infiltrative). These results suggest that the Fast Raman technique is a promising tool to provide an objective diagnosis “tumour clear yes/no” during Mohs surgery of BCC. This clinical integration study is a key step towards a larger scale diagnosis test accuracy study to reliably determine the sensitivity and specificity in a clinical setting. Optical Society of America 2021-03-11 /pmc/articles/PMC8086475/ /pubmed/33996213 http://dx.doi.org/10.1364/BOE.417896 Text en Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. https://creativecommons.org/licenses/by/4.0/https://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Boitor, Radu de Wolf, Coen Weesie, Frank Shipp, Dustin W. Varma, Sandeep Veitch, David Wernham, Aaron Koloydenko, Alexey Puppels, Gerwin Nijsten, Tamar Williams, Hywel C. Caspers, Peter Notingher, Ioan Clinical integration of fast Raman spectroscopy for Mohs micrographic surgery of basal cell carcinoma |
title | Clinical integration of fast Raman spectroscopy for Mohs micrographic surgery of basal cell carcinoma |
title_full | Clinical integration of fast Raman spectroscopy for Mohs micrographic surgery of basal cell carcinoma |
title_fullStr | Clinical integration of fast Raman spectroscopy for Mohs micrographic surgery of basal cell carcinoma |
title_full_unstemmed | Clinical integration of fast Raman spectroscopy for Mohs micrographic surgery of basal cell carcinoma |
title_short | Clinical integration of fast Raman spectroscopy for Mohs micrographic surgery of basal cell carcinoma |
title_sort | clinical integration of fast raman spectroscopy for mohs micrographic surgery of basal cell carcinoma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086475/ https://www.ncbi.nlm.nih.gov/pubmed/33996213 http://dx.doi.org/10.1364/BOE.417896 |
work_keys_str_mv | AT boitorradu clinicalintegrationoffastramanspectroscopyformohsmicrographicsurgeryofbasalcellcarcinoma AT dewolfcoen clinicalintegrationoffastramanspectroscopyformohsmicrographicsurgeryofbasalcellcarcinoma AT weesiefrank clinicalintegrationoffastramanspectroscopyformohsmicrographicsurgeryofbasalcellcarcinoma AT shippdustinw clinicalintegrationoffastramanspectroscopyformohsmicrographicsurgeryofbasalcellcarcinoma AT varmasandeep clinicalintegrationoffastramanspectroscopyformohsmicrographicsurgeryofbasalcellcarcinoma AT veitchdavid clinicalintegrationoffastramanspectroscopyformohsmicrographicsurgeryofbasalcellcarcinoma AT wernhamaaron clinicalintegrationoffastramanspectroscopyformohsmicrographicsurgeryofbasalcellcarcinoma AT koloydenkoalexey clinicalintegrationoffastramanspectroscopyformohsmicrographicsurgeryofbasalcellcarcinoma AT puppelsgerwin clinicalintegrationoffastramanspectroscopyformohsmicrographicsurgeryofbasalcellcarcinoma AT nijstentamar clinicalintegrationoffastramanspectroscopyformohsmicrographicsurgeryofbasalcellcarcinoma AT williamshywelc clinicalintegrationoffastramanspectroscopyformohsmicrographicsurgeryofbasalcellcarcinoma AT casperspeter clinicalintegrationoffastramanspectroscopyformohsmicrographicsurgeryofbasalcellcarcinoma AT notingherioan clinicalintegrationoffastramanspectroscopyformohsmicrographicsurgeryofbasalcellcarcinoma |