A Python script to merge Sanger sequences

Merging Sanger sequences is frequently needed during the gene cloning process. In this study, we provide a Python script that is able to assemble multiple overlapping Sanger sequences. The script utilizes the overlapping regions within the tandem Sanger sequences to merge the Sanger sequences. The r...

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Detalles Bibliográficos
Autores principales: Chen, Cen, Lu, Bingguo, Huang, Xiaofang, Bi, Chuyun, Zhao, Lili, Hu, Yunzhuo, Chen, Xuanyang, Lin, Shiqiang, Huang, Kai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2021
Materias:
Bioinformatics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086567/
https://www.ncbi.nlm.nih.gov/pubmed/33987023
http://dx.doi.org/10.7717/peerj.11354
Descripción
Sumario:Merging Sanger sequences is frequently needed during the gene cloning process. In this study, we provide a Python script that is able to assemble multiple overlapping Sanger sequences. The script utilizes the overlapping regions within the tandem Sanger sequences to merge the Sanger sequences. The results demonstrate that the script can produce the merged sequence from the input Sanger sequences in a single run. The script offers a simple and free method for merging Sanger sequences and is useful for gene cloning.

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