A Python script to merge Sanger sequences

Merging Sanger sequences is frequently needed during the gene cloning process. In this study, we provide a Python script that is able to assemble multiple overlapping Sanger sequences. The script utilizes the overlapping regions within the tandem Sanger sequences to merge the Sanger sequences. The r...

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Detalles Bibliográficos
Autores principales: Chen, Cen, Lu, Bingguo, Huang, Xiaofang, Bi, Chuyun, Zhao, Lili, Hu, Yunzhuo, Chen, Xuanyang, Lin, Shiqiang, Huang, Kai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2021
Materias:
Bioinformatics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086567/
https://www.ncbi.nlm.nih.gov/pubmed/33987023
http://dx.doi.org/10.7717/peerj.11354
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author Chen, Cen
Lu, Bingguo
Huang, Xiaofang
Bi, Chuyun
Zhao, Lili
Hu, Yunzhuo
Chen, Xuanyang
Lin, Shiqiang
Huang, Kai
author_facet Chen, Cen
Lu, Bingguo
Huang, Xiaofang
Bi, Chuyun
Zhao, Lili
Hu, Yunzhuo
Chen, Xuanyang
Lin, Shiqiang
Huang, Kai
author_sort Chen, Cen
collection PubMed
description Merging Sanger sequences is frequently needed during the gene cloning process. In this study, we provide a Python script that is able to assemble multiple overlapping Sanger sequences. The script utilizes the overlapping regions within the tandem Sanger sequences to merge the Sanger sequences. The results demonstrate that the script can produce the merged sequence from the input Sanger sequences in a single run. The script offers a simple and free method for merging Sanger sequences and is useful for gene cloning.
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spelling pubmed-80865672021-05-12 A Python script to merge Sanger sequences Chen, Cen Lu, Bingguo Huang, Xiaofang Bi, Chuyun Zhao, Lili Hu, Yunzhuo Chen, Xuanyang Lin, Shiqiang Huang, Kai PeerJ Bioinformatics Merging Sanger sequences is frequently needed during the gene cloning process. In this study, we provide a Python script that is able to assemble multiple overlapping Sanger sequences. The script utilizes the overlapping regions within the tandem Sanger sequences to merge the Sanger sequences. The results demonstrate that the script can produce the merged sequence from the input Sanger sequences in a single run. The script offers a simple and free method for merging Sanger sequences and is useful for gene cloning. PeerJ Inc. 2021-04-27 /pmc/articles/PMC8086567/ /pubmed/33987023 http://dx.doi.org/10.7717/peerj.11354 Text en © 2021 Chen et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Bioinformatics
Chen, Cen
Lu, Bingguo
Huang, Xiaofang
Bi, Chuyun
Zhao, Lili
Hu, Yunzhuo
Chen, Xuanyang
Lin, Shiqiang
Huang, Kai
A Python script to merge Sanger sequences
title A Python script to merge Sanger sequences
title_full A Python script to merge Sanger sequences
title_fullStr A Python script to merge Sanger sequences
title_full_unstemmed A Python script to merge Sanger sequences
title_short A Python script to merge Sanger sequences
title_sort python script to merge sanger sequences
topic Bioinformatics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086567/
https://www.ncbi.nlm.nih.gov/pubmed/33987023
http://dx.doi.org/10.7717/peerj.11354
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