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Phenotypic, molecular and pathogenic characterization of Colletotrichum scovillei infecting Capsicum species in Rio de Janeiro, Brazil

Anthracnose is a disease caused by Colletotrichum spp., one of the world’s most damaging sweet and chili pepper pathogens, especially in tropical and subtropical regions. In the state of Rio de Janeiro, anthracnose is one of the main obstacles for pepper crops. However, to date no research has focus...

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Autores principales: Giacomin, Renata Mussoi, Ruas, Claudete de Fátima, Baba, Viviane Yumi, De Godoy, Sara Mataroli, Sudré, Claudia Pombo, Bento, Cintia dos Santos, Da Cunha, Maura, Da Costa Geronimo, Ingrid Gaspar, Rodrigues, Rosana, Gonçalves, Leandro SA
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086587/
https://www.ncbi.nlm.nih.gov/pubmed/33986974
http://dx.doi.org/10.7717/peerj.10782
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author Giacomin, Renata Mussoi
Ruas, Claudete de Fátima
Baba, Viviane Yumi
De Godoy, Sara Mataroli
Sudré, Claudia Pombo
Bento, Cintia dos Santos
Da Cunha, Maura
Da Costa Geronimo, Ingrid Gaspar
Rodrigues, Rosana
Gonçalves, Leandro SA
author_facet Giacomin, Renata Mussoi
Ruas, Claudete de Fátima
Baba, Viviane Yumi
De Godoy, Sara Mataroli
Sudré, Claudia Pombo
Bento, Cintia dos Santos
Da Cunha, Maura
Da Costa Geronimo, Ingrid Gaspar
Rodrigues, Rosana
Gonçalves, Leandro SA
author_sort Giacomin, Renata Mussoi
collection PubMed
description Anthracnose is a disease caused by Colletotrichum spp., one of the world’s most damaging sweet and chili pepper pathogens, especially in tropical and subtropical regions. In the state of Rio de Janeiro, anthracnose is one of the main obstacles for pepper crops. However, to date no research has focused on the identification and characterization of the pathogen, which is fundamental to understand the scope of the disease in the state. Thus, the correct identification of the fungal species and pathogenicity studies can provide important support for disease management and control, apart from identifying possible resistance sources for exploitation in peppers breeding programs. In this study, 11 Colletotrichum isolates were collected from peppers with typical symptoms in the Rio de Janeiro state. These isolates were characterized based on morpho-cultural characteristics and sequencing data from five regions (ITS, ACT, CAL, β-TUB and GAPDH), and the genetic variability was estimated by AFLP markers. Simultaneously, microscopy images of the colonization by the fungal species on unripe Capsicum annuum fruits were taken. Pathogenicity was tested and resistance sources were sought by means of infection of ripe and unripe fruits of 50 Capsicum baccatum accessions. The resulting data showed that all isolates belong to Colletotrichum scovillei specie. About the pathogenicity of Capsicum baccatum, differentiated, stage-specific responses, with higher resistance of ripe fruits were recorded. In addition, four possible sources of Colletotrichum scovillei resistance were detected among the tested accessions. The combination of these data can contribute to future studies on the interaction of Colletotrichum scovillei-Capsicum spp., a research line that is still unexploited in the main areas of this anthracnose fungus.
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spelling pubmed-80865872021-05-12 Phenotypic, molecular and pathogenic characterization of Colletotrichum scovillei infecting Capsicum species in Rio de Janeiro, Brazil Giacomin, Renata Mussoi Ruas, Claudete de Fátima Baba, Viviane Yumi De Godoy, Sara Mataroli Sudré, Claudia Pombo Bento, Cintia dos Santos Da Cunha, Maura Da Costa Geronimo, Ingrid Gaspar Rodrigues, Rosana Gonçalves, Leandro SA PeerJ Agricultural Science Anthracnose is a disease caused by Colletotrichum spp., one of the world’s most damaging sweet and chili pepper pathogens, especially in tropical and subtropical regions. In the state of Rio de Janeiro, anthracnose is one of the main obstacles for pepper crops. However, to date no research has focused on the identification and characterization of the pathogen, which is fundamental to understand the scope of the disease in the state. Thus, the correct identification of the fungal species and pathogenicity studies can provide important support for disease management and control, apart from identifying possible resistance sources for exploitation in peppers breeding programs. In this study, 11 Colletotrichum isolates were collected from peppers with typical symptoms in the Rio de Janeiro state. These isolates were characterized based on morpho-cultural characteristics and sequencing data from five regions (ITS, ACT, CAL, β-TUB and GAPDH), and the genetic variability was estimated by AFLP markers. Simultaneously, microscopy images of the colonization by the fungal species on unripe Capsicum annuum fruits were taken. Pathogenicity was tested and resistance sources were sought by means of infection of ripe and unripe fruits of 50 Capsicum baccatum accessions. The resulting data showed that all isolates belong to Colletotrichum scovillei specie. About the pathogenicity of Capsicum baccatum, differentiated, stage-specific responses, with higher resistance of ripe fruits were recorded. In addition, four possible sources of Colletotrichum scovillei resistance were detected among the tested accessions. The combination of these data can contribute to future studies on the interaction of Colletotrichum scovillei-Capsicum spp., a research line that is still unexploited in the main areas of this anthracnose fungus. PeerJ Inc. 2021-04-27 /pmc/articles/PMC8086587/ /pubmed/33986974 http://dx.doi.org/10.7717/peerj.10782 Text en © 2021 Giacomin et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Agricultural Science
Giacomin, Renata Mussoi
Ruas, Claudete de Fátima
Baba, Viviane Yumi
De Godoy, Sara Mataroli
Sudré, Claudia Pombo
Bento, Cintia dos Santos
Da Cunha, Maura
Da Costa Geronimo, Ingrid Gaspar
Rodrigues, Rosana
Gonçalves, Leandro SA
Phenotypic, molecular and pathogenic characterization of Colletotrichum scovillei infecting Capsicum species in Rio de Janeiro, Brazil
title Phenotypic, molecular and pathogenic characterization of Colletotrichum scovillei infecting Capsicum species in Rio de Janeiro, Brazil
title_full Phenotypic, molecular and pathogenic characterization of Colletotrichum scovillei infecting Capsicum species in Rio de Janeiro, Brazil
title_fullStr Phenotypic, molecular and pathogenic characterization of Colletotrichum scovillei infecting Capsicum species in Rio de Janeiro, Brazil
title_full_unstemmed Phenotypic, molecular and pathogenic characterization of Colletotrichum scovillei infecting Capsicum species in Rio de Janeiro, Brazil
title_short Phenotypic, molecular and pathogenic characterization of Colletotrichum scovillei infecting Capsicum species in Rio de Janeiro, Brazil
title_sort phenotypic, molecular and pathogenic characterization of colletotrichum scovillei infecting capsicum species in rio de janeiro, brazil
topic Agricultural Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086587/
https://www.ncbi.nlm.nih.gov/pubmed/33986974
http://dx.doi.org/10.7717/peerj.10782
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