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Facile production of chlorophyllides using recombinant CrCLH1 and their cytotoxicity towards multidrug resistant breast cancer cell lines

The purity of chlorophylls plays one of the key role for the production of chlorophyllides. We have designed a facile method for chlorophyll purification by twice solvent extraction. Twice extraction causes the loss of chlorophylls, but the purity of total chlorophylls can be enhanced 182%. Then, th...

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Autores principales: Hsiang, Yi-Ping, Wang, Yi-Ting, Huang, Keng-Shiang, Huang, Ting-Yu, Tai, Mi-Hsueh, Lin, Yu-Mei, Yang, Chih-Hui, Shaw, Jei-Fu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087012/
https://www.ncbi.nlm.nih.gov/pubmed/33930043
http://dx.doi.org/10.1371/journal.pone.0250565
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author Hsiang, Yi-Ping
Wang, Yi-Ting
Huang, Keng-Shiang
Huang, Ting-Yu
Tai, Mi-Hsueh
Lin, Yu-Mei
Yang, Chih-Hui
Shaw, Jei-Fu
author_facet Hsiang, Yi-Ping
Wang, Yi-Ting
Huang, Keng-Shiang
Huang, Ting-Yu
Tai, Mi-Hsueh
Lin, Yu-Mei
Yang, Chih-Hui
Shaw, Jei-Fu
author_sort Hsiang, Yi-Ping
collection PubMed
description The purity of chlorophylls plays one of the key role for the production of chlorophyllides. We have designed a facile method for chlorophyll purification by twice solvent extraction. Twice extraction causes the loss of chlorophylls, but the purity of total chlorophylls can be enhanced 182%. Then, the purified chlorophylls can be converted to relatively pure chlorophyllides facilely. The results show that higher purity of chlorophyllides could be obtained when purified chlorophylls (ethanol-hexane extract) was used as starting materials than that of crude chlorophylls (ethanol-only extract). In biocompatibility test, the results showed that the prepared chlorophyllides can be applied as biomaterials. When the prepared chlorophyllides were applied to anticancer tests, they were active both in MCF7 and MDA-MB-231 (multidrug resistant breast cancer cells) cell lines. In addition, the results suggested that the prepared chlorophyllides could be a potential candidate of combination therapy with doxorubicin to breast cancers.
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spelling pubmed-80870122021-05-06 Facile production of chlorophyllides using recombinant CrCLH1 and their cytotoxicity towards multidrug resistant breast cancer cell lines Hsiang, Yi-Ping Wang, Yi-Ting Huang, Keng-Shiang Huang, Ting-Yu Tai, Mi-Hsueh Lin, Yu-Mei Yang, Chih-Hui Shaw, Jei-Fu PLoS One Research Article The purity of chlorophylls plays one of the key role for the production of chlorophyllides. We have designed a facile method for chlorophyll purification by twice solvent extraction. Twice extraction causes the loss of chlorophylls, but the purity of total chlorophylls can be enhanced 182%. Then, the purified chlorophylls can be converted to relatively pure chlorophyllides facilely. The results show that higher purity of chlorophyllides could be obtained when purified chlorophylls (ethanol-hexane extract) was used as starting materials than that of crude chlorophylls (ethanol-only extract). In biocompatibility test, the results showed that the prepared chlorophyllides can be applied as biomaterials. When the prepared chlorophyllides were applied to anticancer tests, they were active both in MCF7 and MDA-MB-231 (multidrug resistant breast cancer cells) cell lines. In addition, the results suggested that the prepared chlorophyllides could be a potential candidate of combination therapy with doxorubicin to breast cancers. Public Library of Science 2021-04-30 /pmc/articles/PMC8087012/ /pubmed/33930043 http://dx.doi.org/10.1371/journal.pone.0250565 Text en © 2021 Hsiang et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Hsiang, Yi-Ping
Wang, Yi-Ting
Huang, Keng-Shiang
Huang, Ting-Yu
Tai, Mi-Hsueh
Lin, Yu-Mei
Yang, Chih-Hui
Shaw, Jei-Fu
Facile production of chlorophyllides using recombinant CrCLH1 and their cytotoxicity towards multidrug resistant breast cancer cell lines
title Facile production of chlorophyllides using recombinant CrCLH1 and their cytotoxicity towards multidrug resistant breast cancer cell lines
title_full Facile production of chlorophyllides using recombinant CrCLH1 and their cytotoxicity towards multidrug resistant breast cancer cell lines
title_fullStr Facile production of chlorophyllides using recombinant CrCLH1 and their cytotoxicity towards multidrug resistant breast cancer cell lines
title_full_unstemmed Facile production of chlorophyllides using recombinant CrCLH1 and their cytotoxicity towards multidrug resistant breast cancer cell lines
title_short Facile production of chlorophyllides using recombinant CrCLH1 and their cytotoxicity towards multidrug resistant breast cancer cell lines
title_sort facile production of chlorophyllides using recombinant crclh1 and their cytotoxicity towards multidrug resistant breast cancer cell lines
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087012/
https://www.ncbi.nlm.nih.gov/pubmed/33930043
http://dx.doi.org/10.1371/journal.pone.0250565
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