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Microwave-Assisted Chitosan-Functionalized Graphene Oxide as Controlled Intracellular Drug Delivery Nanosystem for Synergistic Antitumour Activity

To achieve better antitumour efficacy, it is urgent to improve anticancer drug delivery efficiency in targeting cancer cells. In this work, chitosan-functionalized graphene oxide (ChrGO) nanosheets were fabricated via microwave-assisted reduction, which were employed to the intracellular delivery na...

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Autores principales: Shu, Mengjun, Gao, Feng, Zeng, Min, Yu, Chulang, Wang, Xue, Huang, Renhua, Yang, Jianhua, Su, Yanjie, Hu, Nantao, Zhou, Zhihua, Liu, Ke, Yang, Zhi, Tan, Hongtao, Xu, Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087749/
https://www.ncbi.nlm.nih.gov/pubmed/33929622
http://dx.doi.org/10.1186/s11671-021-03525-y
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author Shu, Mengjun
Gao, Feng
Zeng, Min
Yu, Chulang
Wang, Xue
Huang, Renhua
Yang, Jianhua
Su, Yanjie
Hu, Nantao
Zhou, Zhihua
Liu, Ke
Yang, Zhi
Tan, Hongtao
Xu, Lin
author_facet Shu, Mengjun
Gao, Feng
Zeng, Min
Yu, Chulang
Wang, Xue
Huang, Renhua
Yang, Jianhua
Su, Yanjie
Hu, Nantao
Zhou, Zhihua
Liu, Ke
Yang, Zhi
Tan, Hongtao
Xu, Lin
author_sort Shu, Mengjun
collection PubMed
description To achieve better antitumour efficacy, it is urgent to improve anticancer drug delivery efficiency in targeting cancer cells. In this work, chitosan-functionalized graphene oxide (ChrGO) nanosheets were fabricated via microwave-assisted reduction, which were employed to the intracellular delivery nanosystem for anticancer drug agent in breast cancer cells. Drug loading and release research indicated that adriamycin can be efficiently loaded on and released from the ChrGO nanosheets. Less drug release during delivery and better biocompatibility of ChrGO/adriamycin significantly improve its safety and therapeutic efficacy in HER2-overexpressing BT-474 cells. Furthermore, ChrGO/adriamycin in combination with trastuzumab exhibited synergistic antitumour activity in BT-474 cells, which demonstrated superior therapeutic efficacy compared with each drug alone. Cells treated with trastuzumab (5 μg/mL) or equivalent ChrGO/adriamycin (5 μg/mL) each elicited 54.5% and 59.5% cell death, respectively, while the combination treatment with trastuzumab and ChrGO/adriamycin resulted in a dramatic 88.5% cell death. The dual-targeted therapy displayed higher apoptosis, indicating superior therapeutic efficacy due to the presence of different mechanisms of action. The combined treatment of ChrGO/adriamycin and trastuzumab in BT-474 cells induced cell cycle arrest and apoptosis, which ultimately led to the death of augmented cancer cells. This work has provided a facile microwave-assisted fabrication of ChrGO as a controlled and targeted intracellular drug delivery nanosystem, which is expected to be a novel promising therapy for treating HER2-overexpressing breast cancer cells. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s11671-021-03525-y.
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spelling pubmed-80877492021-05-05 Microwave-Assisted Chitosan-Functionalized Graphene Oxide as Controlled Intracellular Drug Delivery Nanosystem for Synergistic Antitumour Activity Shu, Mengjun Gao, Feng Zeng, Min Yu, Chulang Wang, Xue Huang, Renhua Yang, Jianhua Su, Yanjie Hu, Nantao Zhou, Zhihua Liu, Ke Yang, Zhi Tan, Hongtao Xu, Lin Nanoscale Res Lett Nano Express To achieve better antitumour efficacy, it is urgent to improve anticancer drug delivery efficiency in targeting cancer cells. In this work, chitosan-functionalized graphene oxide (ChrGO) nanosheets were fabricated via microwave-assisted reduction, which were employed to the intracellular delivery nanosystem for anticancer drug agent in breast cancer cells. Drug loading and release research indicated that adriamycin can be efficiently loaded on and released from the ChrGO nanosheets. Less drug release during delivery and better biocompatibility of ChrGO/adriamycin significantly improve its safety and therapeutic efficacy in HER2-overexpressing BT-474 cells. Furthermore, ChrGO/adriamycin in combination with trastuzumab exhibited synergistic antitumour activity in BT-474 cells, which demonstrated superior therapeutic efficacy compared with each drug alone. Cells treated with trastuzumab (5 μg/mL) or equivalent ChrGO/adriamycin (5 μg/mL) each elicited 54.5% and 59.5% cell death, respectively, while the combination treatment with trastuzumab and ChrGO/adriamycin resulted in a dramatic 88.5% cell death. The dual-targeted therapy displayed higher apoptosis, indicating superior therapeutic efficacy due to the presence of different mechanisms of action. The combined treatment of ChrGO/adriamycin and trastuzumab in BT-474 cells induced cell cycle arrest and apoptosis, which ultimately led to the death of augmented cancer cells. This work has provided a facile microwave-assisted fabrication of ChrGO as a controlled and targeted intracellular drug delivery nanosystem, which is expected to be a novel promising therapy for treating HER2-overexpressing breast cancer cells. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s11671-021-03525-y. Springer US 2021-04-30 /pmc/articles/PMC8087749/ /pubmed/33929622 http://dx.doi.org/10.1186/s11671-021-03525-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Nano Express
Shu, Mengjun
Gao, Feng
Zeng, Min
Yu, Chulang
Wang, Xue
Huang, Renhua
Yang, Jianhua
Su, Yanjie
Hu, Nantao
Zhou, Zhihua
Liu, Ke
Yang, Zhi
Tan, Hongtao
Xu, Lin
Microwave-Assisted Chitosan-Functionalized Graphene Oxide as Controlled Intracellular Drug Delivery Nanosystem for Synergistic Antitumour Activity
title Microwave-Assisted Chitosan-Functionalized Graphene Oxide as Controlled Intracellular Drug Delivery Nanosystem for Synergistic Antitumour Activity
title_full Microwave-Assisted Chitosan-Functionalized Graphene Oxide as Controlled Intracellular Drug Delivery Nanosystem for Synergistic Antitumour Activity
title_fullStr Microwave-Assisted Chitosan-Functionalized Graphene Oxide as Controlled Intracellular Drug Delivery Nanosystem for Synergistic Antitumour Activity
title_full_unstemmed Microwave-Assisted Chitosan-Functionalized Graphene Oxide as Controlled Intracellular Drug Delivery Nanosystem for Synergistic Antitumour Activity
title_short Microwave-Assisted Chitosan-Functionalized Graphene Oxide as Controlled Intracellular Drug Delivery Nanosystem for Synergistic Antitumour Activity
title_sort microwave-assisted chitosan-functionalized graphene oxide as controlled intracellular drug delivery nanosystem for synergistic antitumour activity
topic Nano Express
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087749/
https://www.ncbi.nlm.nih.gov/pubmed/33929622
http://dx.doi.org/10.1186/s11671-021-03525-y
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