Cargando…
Developing Nanodisc-ID for label-free characterizations of membrane proteins
Membrane proteins (MPs) influence all aspects of life, such as tumorigenesis, immune response, and neural transmission. However, characterization of MPs is challenging, as it often needs highly specialized techniques inaccessible to many labs. We herein introduce nanodisc-ID that enables quantitativ...
Autor principal: | |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087782/ https://www.ncbi.nlm.nih.gov/pubmed/33931748 http://dx.doi.org/10.1038/s42003-021-02043-y |
_version_ | 1783686725022777344 |
---|---|
author | Bao, Huan |
author_facet | Bao, Huan |
author_sort | Bao, Huan |
collection | PubMed |
description | Membrane proteins (MPs) influence all aspects of life, such as tumorigenesis, immune response, and neural transmission. However, characterization of MPs is challenging, as it often needs highly specialized techniques inaccessible to many labs. We herein introduce nanodisc-ID that enables quantitative analysis of membrane proteins using a gel electrophoresis readout. By leveraging the power of nanodiscs and proximity labeling, nanodisc-ID serves both as scaffolds for encasing biochemical reactions and as sensitive reagents for detecting membrane protein-lipid and protein-protein interactions. We demonstrate this label-free and low-cost tool by characterizing a wide range of integral and peripheral membrane proteins from prokaryotes and eukaryotes. |
format | Online Article Text |
id | pubmed-8087782 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-80877822021-05-05 Developing Nanodisc-ID for label-free characterizations of membrane proteins Bao, Huan Commun Biol Article Membrane proteins (MPs) influence all aspects of life, such as tumorigenesis, immune response, and neural transmission. However, characterization of MPs is challenging, as it often needs highly specialized techniques inaccessible to many labs. We herein introduce nanodisc-ID that enables quantitative analysis of membrane proteins using a gel electrophoresis readout. By leveraging the power of nanodiscs and proximity labeling, nanodisc-ID serves both as scaffolds for encasing biochemical reactions and as sensitive reagents for detecting membrane protein-lipid and protein-protein interactions. We demonstrate this label-free and low-cost tool by characterizing a wide range of integral and peripheral membrane proteins from prokaryotes and eukaryotes. Nature Publishing Group UK 2021-04-30 /pmc/articles/PMC8087782/ /pubmed/33931748 http://dx.doi.org/10.1038/s42003-021-02043-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Bao, Huan Developing Nanodisc-ID for label-free characterizations of membrane proteins |
title | Developing Nanodisc-ID for label-free characterizations of membrane proteins |
title_full | Developing Nanodisc-ID for label-free characterizations of membrane proteins |
title_fullStr | Developing Nanodisc-ID for label-free characterizations of membrane proteins |
title_full_unstemmed | Developing Nanodisc-ID for label-free characterizations of membrane proteins |
title_short | Developing Nanodisc-ID for label-free characterizations of membrane proteins |
title_sort | developing nanodisc-id for label-free characterizations of membrane proteins |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087782/ https://www.ncbi.nlm.nih.gov/pubmed/33931748 http://dx.doi.org/10.1038/s42003-021-02043-y |
work_keys_str_mv | AT baohuan developingnanodiscidforlabelfreecharacterizationsofmembraneproteins |