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Influence of high‐dose antithrombin on platelet function and blood coagulation

AIM: In healthy adults, there are sufficient amounts of antithrombin in the blood to regulate thrombin. However, the effects of high concentrations of antithrombin on dose‐dependent anticoagulation and platelet function have not been reported. In this study, we assessed platelet function and blood c...

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Autores principales: Miike, Toru, Sakamoto, Yuichiro, Narumi, Shougo, Yoshitake, Kunimasa, Sakurai, Ryota, Nakayama, Kento, Inoue, Satoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8088397/
https://www.ncbi.nlm.nih.gov/pubmed/33968412
http://dx.doi.org/10.1002/ams2.648
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author Miike, Toru
Sakamoto, Yuichiro
Narumi, Shougo
Yoshitake, Kunimasa
Sakurai, Ryota
Nakayama, Kento
Inoue, Satoshi
author_facet Miike, Toru
Sakamoto, Yuichiro
Narumi, Shougo
Yoshitake, Kunimasa
Sakurai, Ryota
Nakayama, Kento
Inoue, Satoshi
author_sort Miike, Toru
collection PubMed
description AIM: In healthy adults, there are sufficient amounts of antithrombin in the blood to regulate thrombin. However, the effects of high concentrations of antithrombin on dose‐dependent anticoagulation and platelet function have not been reported. In this study, we assessed platelet function and blood coagulation following high‐dose antithrombin supplementation in vitro. METHODS: Blood samples were collected from 10 healthy volunteers, and samples with different antithrombin concentrations were prepared by adding an antithrombin agent (Neuart). Blood coagulation was assessed by the Thrombus‐Formation Analysis System (T‐TAS) and Rotational Thromboelastometry (ROTEM) using whole blood samples. RESULTS: The data obtained by the platelet chip, exclusively representing platelet function, revealed that the onset of thrombus formation was significantly delayed in a dose‐dependent manner (100%–200%, P = 0.021; 100%–500%, P = 0.011; 200%–500%, P = 0.047). In measurements using the atheroma chip, which enables assessment of blood coagulation, the thrombus formation ability was found to be reduced (100%–200%, P = 0.022; 100%–500%, P = 0.05). In the ROTEM measurements, clotting time was prolonged in a dose‐dependent manner (100%–200%: P = 0.203, 200%–500%: P = 0.005, 500%–1000%: P = 0.022), except when comparing with 100% and 200%. Although antithrombin is reportedly saturated in healthy blood, its anticoagulant ability appears to be enhanced depending on its concentration. Furthermore, data obtained from the platelet chip showed that antithrombin might reduce platelet function. CONCLUSIONS: Antithrombin suppressed platelet function and blood coagulation in a dose‐dependent manner.
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spelling pubmed-80883972021-05-07 Influence of high‐dose antithrombin on platelet function and blood coagulation Miike, Toru Sakamoto, Yuichiro Narumi, Shougo Yoshitake, Kunimasa Sakurai, Ryota Nakayama, Kento Inoue, Satoshi Acute Med Surg Original Articles AIM: In healthy adults, there are sufficient amounts of antithrombin in the blood to regulate thrombin. However, the effects of high concentrations of antithrombin on dose‐dependent anticoagulation and platelet function have not been reported. In this study, we assessed platelet function and blood coagulation following high‐dose antithrombin supplementation in vitro. METHODS: Blood samples were collected from 10 healthy volunteers, and samples with different antithrombin concentrations were prepared by adding an antithrombin agent (Neuart). Blood coagulation was assessed by the Thrombus‐Formation Analysis System (T‐TAS) and Rotational Thromboelastometry (ROTEM) using whole blood samples. RESULTS: The data obtained by the platelet chip, exclusively representing platelet function, revealed that the onset of thrombus formation was significantly delayed in a dose‐dependent manner (100%–200%, P = 0.021; 100%–500%, P = 0.011; 200%–500%, P = 0.047). In measurements using the atheroma chip, which enables assessment of blood coagulation, the thrombus formation ability was found to be reduced (100%–200%, P = 0.022; 100%–500%, P = 0.05). In the ROTEM measurements, clotting time was prolonged in a dose‐dependent manner (100%–200%: P = 0.203, 200%–500%: P = 0.005, 500%–1000%: P = 0.022), except when comparing with 100% and 200%. Although antithrombin is reportedly saturated in healthy blood, its anticoagulant ability appears to be enhanced depending on its concentration. Furthermore, data obtained from the platelet chip showed that antithrombin might reduce platelet function. CONCLUSIONS: Antithrombin suppressed platelet function and blood coagulation in a dose‐dependent manner. John Wiley and Sons Inc. 2021-05-01 /pmc/articles/PMC8088397/ /pubmed/33968412 http://dx.doi.org/10.1002/ams2.648 Text en © 2021 The Authors. Acute Medicine & Surgery published by John Wiley & Sons Australia, Ltd on behalf of Japanese Association for Acute Medicine https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Articles
Miike, Toru
Sakamoto, Yuichiro
Narumi, Shougo
Yoshitake, Kunimasa
Sakurai, Ryota
Nakayama, Kento
Inoue, Satoshi
Influence of high‐dose antithrombin on platelet function and blood coagulation
title Influence of high‐dose antithrombin on platelet function and blood coagulation
title_full Influence of high‐dose antithrombin on platelet function and blood coagulation
title_fullStr Influence of high‐dose antithrombin on platelet function and blood coagulation
title_full_unstemmed Influence of high‐dose antithrombin on platelet function and blood coagulation
title_short Influence of high‐dose antithrombin on platelet function and blood coagulation
title_sort influence of high‐dose antithrombin on platelet function and blood coagulation
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8088397/
https://www.ncbi.nlm.nih.gov/pubmed/33968412
http://dx.doi.org/10.1002/ams2.648
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