Cargando…
The effects of melatonin treatment on oxidative stress induced by ovariohysterectomy in dogs
BACKGROUND: As one of the most common surgeries performed in veterinary medicine, ovariohysterectomy (OHE) can induce oxidative stress in dogs. The antioxidant properties of melatonin have been confirmed in various studies. This study aimed to investigate the effects of melatonin administration on o...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8088575/ https://www.ncbi.nlm.nih.gov/pubmed/33933076 http://dx.doi.org/10.1186/s12917-021-02882-1 |
_version_ | 1783686872072978432 |
---|---|
author | Salavati, Sina Mogheiseh, Asghar Nazifi, Saeed Amiri, Atefeh Nikahval, Behrooz |
author_facet | Salavati, Sina Mogheiseh, Asghar Nazifi, Saeed Amiri, Atefeh Nikahval, Behrooz |
author_sort | Salavati, Sina |
collection | PubMed |
description | BACKGROUND: As one of the most common surgeries performed in veterinary medicine, ovariohysterectomy (OHE) can induce oxidative stress in dogs. The antioxidant properties of melatonin have been confirmed in various studies. This study aimed to investigate the effects of melatonin administration on oxidative stress in dogs before and after OHE. In this study, 25 mature female intact dogs were selected and randomly divided into five equal groups: Melatonin (melatonin, no surgery), OHE (no melatonin, surgery), OHE + melatonin (melatonin, surgery), anesthesia+melatonin (melatonin, sham surgery), and control (no melatonin, no surgery) groups. Melatonin (0.3 mg/Kg/day, p.o.) was administrated to the dogs in the melatonin, OHE + melatonin, and anesthesia+melatonin groups on days − 1, 0, 1, 2, and 3 (day 0 = OHE). Blood sampling was performed on days − 1, 1, 3, and 5 of the study. Blood samples were immediately transferred to the laboratory and sera were separated and stored at − 20 °C. Superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT) and malondialdehyde (MDA) concentrations were measured with commercial kits. RESULTS: The levels of SOD, GPX and CAT were significantly higher in the melatonin and anesthesia+melatonin groups compared to those of the control group at days 3 and 5. The level of antioxidant enzymes significantly decreased in the OHE group compared to that of other groups at days 3 and 5. The administration of melatonin increased the level of antioxidant enzymes in ovariohysterectomized dogs. Ovariohysterectomy significantly increased the concentration of MDA in comparison to that of other groups at day 3. Melatonin administration significantly decreased the level of MDA in melatonin, anesthetized, and ovariohysterectomized dogs at day 3. CONCLUSIONS: Administration of melatonin on day − 1, 0, 1, 2 and 3 modulate the oxidative stress induced by OHE in dogs by increasing antioxidant enzymes concentration and decreasing MDA levels. |
format | Online Article Text |
id | pubmed-8088575 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-80885752021-05-03 The effects of melatonin treatment on oxidative stress induced by ovariohysterectomy in dogs Salavati, Sina Mogheiseh, Asghar Nazifi, Saeed Amiri, Atefeh Nikahval, Behrooz BMC Vet Res Research Article BACKGROUND: As one of the most common surgeries performed in veterinary medicine, ovariohysterectomy (OHE) can induce oxidative stress in dogs. The antioxidant properties of melatonin have been confirmed in various studies. This study aimed to investigate the effects of melatonin administration on oxidative stress in dogs before and after OHE. In this study, 25 mature female intact dogs were selected and randomly divided into five equal groups: Melatonin (melatonin, no surgery), OHE (no melatonin, surgery), OHE + melatonin (melatonin, surgery), anesthesia+melatonin (melatonin, sham surgery), and control (no melatonin, no surgery) groups. Melatonin (0.3 mg/Kg/day, p.o.) was administrated to the dogs in the melatonin, OHE + melatonin, and anesthesia+melatonin groups on days − 1, 0, 1, 2, and 3 (day 0 = OHE). Blood sampling was performed on days − 1, 1, 3, and 5 of the study. Blood samples were immediately transferred to the laboratory and sera were separated and stored at − 20 °C. Superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT) and malondialdehyde (MDA) concentrations were measured with commercial kits. RESULTS: The levels of SOD, GPX and CAT were significantly higher in the melatonin and anesthesia+melatonin groups compared to those of the control group at days 3 and 5. The level of antioxidant enzymes significantly decreased in the OHE group compared to that of other groups at days 3 and 5. The administration of melatonin increased the level of antioxidant enzymes in ovariohysterectomized dogs. Ovariohysterectomy significantly increased the concentration of MDA in comparison to that of other groups at day 3. Melatonin administration significantly decreased the level of MDA in melatonin, anesthetized, and ovariohysterectomized dogs at day 3. CONCLUSIONS: Administration of melatonin on day − 1, 0, 1, 2 and 3 modulate the oxidative stress induced by OHE in dogs by increasing antioxidant enzymes concentration and decreasing MDA levels. BioMed Central 2021-05-01 /pmc/articles/PMC8088575/ /pubmed/33933076 http://dx.doi.org/10.1186/s12917-021-02882-1 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Salavati, Sina Mogheiseh, Asghar Nazifi, Saeed Amiri, Atefeh Nikahval, Behrooz The effects of melatonin treatment on oxidative stress induced by ovariohysterectomy in dogs |
title | The effects of melatonin treatment on oxidative stress induced by ovariohysterectomy in dogs |
title_full | The effects of melatonin treatment on oxidative stress induced by ovariohysterectomy in dogs |
title_fullStr | The effects of melatonin treatment on oxidative stress induced by ovariohysterectomy in dogs |
title_full_unstemmed | The effects of melatonin treatment on oxidative stress induced by ovariohysterectomy in dogs |
title_short | The effects of melatonin treatment on oxidative stress induced by ovariohysterectomy in dogs |
title_sort | effects of melatonin treatment on oxidative stress induced by ovariohysterectomy in dogs |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8088575/ https://www.ncbi.nlm.nih.gov/pubmed/33933076 http://dx.doi.org/10.1186/s12917-021-02882-1 |
work_keys_str_mv | AT salavatisina theeffectsofmelatonintreatmentonoxidativestressinducedbyovariohysterectomyindogs AT mogheisehasghar theeffectsofmelatonintreatmentonoxidativestressinducedbyovariohysterectomyindogs AT nazifisaeed theeffectsofmelatonintreatmentonoxidativestressinducedbyovariohysterectomyindogs AT amiriatefeh theeffectsofmelatonintreatmentonoxidativestressinducedbyovariohysterectomyindogs AT nikahvalbehrooz theeffectsofmelatonintreatmentonoxidativestressinducedbyovariohysterectomyindogs AT salavatisina effectsofmelatonintreatmentonoxidativestressinducedbyovariohysterectomyindogs AT mogheisehasghar effectsofmelatonintreatmentonoxidativestressinducedbyovariohysterectomyindogs AT nazifisaeed effectsofmelatonintreatmentonoxidativestressinducedbyovariohysterectomyindogs AT amiriatefeh effectsofmelatonintreatmentonoxidativestressinducedbyovariohysterectomyindogs AT nikahvalbehrooz effectsofmelatonintreatmentonoxidativestressinducedbyovariohysterectomyindogs |