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PD‐L1 positively regulates MET phosphorylation through inhibiting PTP1B
Increasing bodies of evidence support the involvement of tumor‐intrinsic action in PD‐L1‐mediated cancer progression. However, the mechanisms underlying the tumor‐intrinsic function of PD‐L1 are less well understood. In the present study, we found a positive correlation between PD‐L1 expression and...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8088939/ https://www.ncbi.nlm.nih.gov/pubmed/33583114 http://dx.doi.org/10.1111/cas.14844 |
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author | Lu, Shangyun Sun, Zhenou Hu, Wenli Yin, Shutao Zhao, Chong Hu, Hongbo |
author_facet | Lu, Shangyun Sun, Zhenou Hu, Wenli Yin, Shutao Zhao, Chong Hu, Hongbo |
author_sort | Lu, Shangyun |
collection | PubMed |
description | Increasing bodies of evidence support the involvement of tumor‐intrinsic action in PD‐L1‐mediated cancer progression. However, the mechanisms underlying the tumor‐intrinsic function of PD‐L1 are less well understood. In the present study, we found a positive correlation between PD‐L1 expression and MET phosphorylation in lung cancer and melanoma cell lines. PD‐L1 inhibition led to a decrease in MET phosphorylation, while PD‐L1 induction by IFN‐γ resulted in a PD‐L1‐dependent increase of MET phosphorylation both in vitro and in vivo. The results indicated that MET phosphorylation can be positively regulated by PD‐L1. Furthermore, we identified PTP1B as a mediator contributing to the regulation of MET phosphorylation by PD‐L1. In agreement with the induction of MET phosphorylation by PD‐L1, inhibition of PD‐L1 caused reduced phosphorylation of ERKs, a known downstream kinase of MET, and inhibited cell proliferation. Collectively, the present study demonstrated for the first time that the MET pathway, as a downstream of PD‐L1, contributed to its tumor‐intrinsic effect, and provided a novel mechanistic explanation for the tumor‐intrinsic function of PD‐L1 and a rationale for the combination of immunotherapy and MET‐targeted therapy in cancer treatment. |
format | Online Article Text |
id | pubmed-8088939 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-80889392021-05-10 PD‐L1 positively regulates MET phosphorylation through inhibiting PTP1B Lu, Shangyun Sun, Zhenou Hu, Wenli Yin, Shutao Zhao, Chong Hu, Hongbo Cancer Sci Original Articles Increasing bodies of evidence support the involvement of tumor‐intrinsic action in PD‐L1‐mediated cancer progression. However, the mechanisms underlying the tumor‐intrinsic function of PD‐L1 are less well understood. In the present study, we found a positive correlation between PD‐L1 expression and MET phosphorylation in lung cancer and melanoma cell lines. PD‐L1 inhibition led to a decrease in MET phosphorylation, while PD‐L1 induction by IFN‐γ resulted in a PD‐L1‐dependent increase of MET phosphorylation both in vitro and in vivo. The results indicated that MET phosphorylation can be positively regulated by PD‐L1. Furthermore, we identified PTP1B as a mediator contributing to the regulation of MET phosphorylation by PD‐L1. In agreement with the induction of MET phosphorylation by PD‐L1, inhibition of PD‐L1 caused reduced phosphorylation of ERKs, a known downstream kinase of MET, and inhibited cell proliferation. Collectively, the present study demonstrated for the first time that the MET pathway, as a downstream of PD‐L1, contributed to its tumor‐intrinsic effect, and provided a novel mechanistic explanation for the tumor‐intrinsic function of PD‐L1 and a rationale for the combination of immunotherapy and MET‐targeted therapy in cancer treatment. John Wiley and Sons Inc. 2021-03-04 2021-05 /pmc/articles/PMC8088939/ /pubmed/33583114 http://dx.doi.org/10.1111/cas.14844 Text en © 2021 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Original Articles Lu, Shangyun Sun, Zhenou Hu, Wenli Yin, Shutao Zhao, Chong Hu, Hongbo PD‐L1 positively regulates MET phosphorylation through inhibiting PTP1B |
title | PD‐L1 positively regulates MET phosphorylation through inhibiting PTP1B |
title_full | PD‐L1 positively regulates MET phosphorylation through inhibiting PTP1B |
title_fullStr | PD‐L1 positively regulates MET phosphorylation through inhibiting PTP1B |
title_full_unstemmed | PD‐L1 positively regulates MET phosphorylation through inhibiting PTP1B |
title_short | PD‐L1 positively regulates MET phosphorylation through inhibiting PTP1B |
title_sort | pd‐l1 positively regulates met phosphorylation through inhibiting ptp1b |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8088939/ https://www.ncbi.nlm.nih.gov/pubmed/33583114 http://dx.doi.org/10.1111/cas.14844 |
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