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Genomic analysis between idiopathic pulmonary fibrosis and associated lung cancer using laser‐assisted microdissection: A case report
Lung cancer (LC) is the most fatal complication of idiopathic pulmonary fibrosis (IPF). However, the molecular pathogenesis of the development of LC from IPF is still unclear. Here, we report a case of IPF‐associated LC for which we investigated the genetic alterations between IPF and LC. We extract...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons Australia, Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8088965/ https://www.ncbi.nlm.nih.gov/pubmed/33784423 http://dx.doi.org/10.1111/1759-7714.13924 |
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author | Iida, Yuko Gon, Yasuhiro Nakanishi, Yoko Kurosawa, Yusuke Nakagawa, Yoshiko Mizumura, Kenji Shimizu, Tetsuo Takahashi, Noriaki Masuda, Shinobu |
author_facet | Iida, Yuko Gon, Yasuhiro Nakanishi, Yoko Kurosawa, Yusuke Nakagawa, Yoshiko Mizumura, Kenji Shimizu, Tetsuo Takahashi, Noriaki Masuda, Shinobu |
author_sort | Iida, Yuko |
collection | PubMed |
description | Lung cancer (LC) is the most fatal complication of idiopathic pulmonary fibrosis (IPF). However, the molecular pathogenesis of the development of LC from IPF is still unclear. Here, we report a case of IPF‐associated LC for which we investigated the genetic alterations between IPF and LC. We extracted formalin‐fixed paraffin‐embedded DNA from each part of the surgical lung tissue using a laser‐assisted microdissection technique. The mutations in each part were detected by next‐generation sequencing (NGS) using 72 lung cancer‐related mutation panels. Five mutations were found in IPF and four in LC. Almost all somatic mutations did not overlap between the IPF and LC regions. These findings suggest that IPF‐associated LC may not be a result of the accumulation of somatic mutations in the regenerated epithelium of the honeycomb lung in the IPF region. |
format | Online Article Text |
id | pubmed-8088965 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley & Sons Australia, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-80889652021-05-10 Genomic analysis between idiopathic pulmonary fibrosis and associated lung cancer using laser‐assisted microdissection: A case report Iida, Yuko Gon, Yasuhiro Nakanishi, Yoko Kurosawa, Yusuke Nakagawa, Yoshiko Mizumura, Kenji Shimizu, Tetsuo Takahashi, Noriaki Masuda, Shinobu Thorac Cancer Case Reports Lung cancer (LC) is the most fatal complication of idiopathic pulmonary fibrosis (IPF). However, the molecular pathogenesis of the development of LC from IPF is still unclear. Here, we report a case of IPF‐associated LC for which we investigated the genetic alterations between IPF and LC. We extracted formalin‐fixed paraffin‐embedded DNA from each part of the surgical lung tissue using a laser‐assisted microdissection technique. The mutations in each part were detected by next‐generation sequencing (NGS) using 72 lung cancer‐related mutation panels. Five mutations were found in IPF and four in LC. Almost all somatic mutations did not overlap between the IPF and LC regions. These findings suggest that IPF‐associated LC may not be a result of the accumulation of somatic mutations in the regenerated epithelium of the honeycomb lung in the IPF region. John Wiley & Sons Australia, Ltd 2021-03-30 2021-05 /pmc/articles/PMC8088965/ /pubmed/33784423 http://dx.doi.org/10.1111/1759-7714.13924 Text en © 2021 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Case Reports Iida, Yuko Gon, Yasuhiro Nakanishi, Yoko Kurosawa, Yusuke Nakagawa, Yoshiko Mizumura, Kenji Shimizu, Tetsuo Takahashi, Noriaki Masuda, Shinobu Genomic analysis between idiopathic pulmonary fibrosis and associated lung cancer using laser‐assisted microdissection: A case report |
title | Genomic analysis between idiopathic pulmonary fibrosis and associated lung cancer using laser‐assisted microdissection: A case report |
title_full | Genomic analysis between idiopathic pulmonary fibrosis and associated lung cancer using laser‐assisted microdissection: A case report |
title_fullStr | Genomic analysis between idiopathic pulmonary fibrosis and associated lung cancer using laser‐assisted microdissection: A case report |
title_full_unstemmed | Genomic analysis between idiopathic pulmonary fibrosis and associated lung cancer using laser‐assisted microdissection: A case report |
title_short | Genomic analysis between idiopathic pulmonary fibrosis and associated lung cancer using laser‐assisted microdissection: A case report |
title_sort | genomic analysis between idiopathic pulmonary fibrosis and associated lung cancer using laser‐assisted microdissection: a case report |
topic | Case Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8088965/ https://www.ncbi.nlm.nih.gov/pubmed/33784423 http://dx.doi.org/10.1111/1759-7714.13924 |
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