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Improved response of human gingival fibroblasts to titanium coated with micro-/nano-structured tantalum
OBJECTIVES: This study aims to evaluate the ability of tantalum-coated titanium to improve human gingival fibroblasts’ adhesion, viability, proliferation, migration performance, and the potential molecular mechanisms. MATERIALS AND METHODS: Titanium plates were divided into two groups: (1) no coatin...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8089072/ https://www.ncbi.nlm.nih.gov/pubmed/33937945 http://dx.doi.org/10.1186/s40729-021-00316-z |
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author | Zhang, Chu-nan Zhou, Lin-yi Qian, Shu-jiao Gu, Ying-xin Shi, Jun-yu Lai, Hong-chang |
author_facet | Zhang, Chu-nan Zhou, Lin-yi Qian, Shu-jiao Gu, Ying-xin Shi, Jun-yu Lai, Hong-chang |
author_sort | Zhang, Chu-nan |
collection | PubMed |
description | OBJECTIVES: This study aims to evaluate the ability of tantalum-coated titanium to improve human gingival fibroblasts’ adhesion, viability, proliferation, migration performance, and the potential molecular mechanisms. MATERIALS AND METHODS: Titanium plates were divided into two groups: (1) no coating (Ti, control), (2) Tantalum-coated titanium (Ta-coated Ti). All samples were characterized by scanning electronic microscopy, surface roughness, and hydrophilicity. Fibroblasts’ performance were analyzed by attached cell number at 1 h, 4 h, and 24 h, morphology at 1 h and 4 h, viability at 1 day, 3 days, 5 days, and 7 days, recovery after wounding at 6 h, 12 h, and 24 h. RT-PCR, western blot were applied to detect attachment-related genes’ expression and protein synthesis at 4 h and 24 h. Student’s t test was used for statistical analysis. RESULTS: Tantalum-coated titanium demonstrates a layer of homogeneously distributed nano-grains with mean diameter of 25.98 (± 14.75) nm. It was found that after tantalum deposition, human gingival fibroblasts (HGFs) adhesion, viability, proliferation, and migration were promoted in comparison to the control group. An upregulated level of Integrin β1 and FAK signaling was also detected, which might be the underlying mechanism. CONCLUSION: In the present study, adhesion, viability, proliferation, migration of human gingival fibroblasts are promoted on tantalum-coated titanium, upregulated integrin β1 and FAK might contribute to its superior performance, indicating tantalum coating can be applied in transmucosal part of dental implant. CLINICAL SIGNIFICANCE: Tantalum deposition on titanium surfaces can promote human gingival fibroblast adhesion, accordingly forming a well-organized soft tissue sealing and may contribute to a successful osseointegration. |
format | Online Article Text |
id | pubmed-8089072 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-80890722021-05-05 Improved response of human gingival fibroblasts to titanium coated with micro-/nano-structured tantalum Zhang, Chu-nan Zhou, Lin-yi Qian, Shu-jiao Gu, Ying-xin Shi, Jun-yu Lai, Hong-chang Int J Implant Dent Research OBJECTIVES: This study aims to evaluate the ability of tantalum-coated titanium to improve human gingival fibroblasts’ adhesion, viability, proliferation, migration performance, and the potential molecular mechanisms. MATERIALS AND METHODS: Titanium plates were divided into two groups: (1) no coating (Ti, control), (2) Tantalum-coated titanium (Ta-coated Ti). All samples were characterized by scanning electronic microscopy, surface roughness, and hydrophilicity. Fibroblasts’ performance were analyzed by attached cell number at 1 h, 4 h, and 24 h, morphology at 1 h and 4 h, viability at 1 day, 3 days, 5 days, and 7 days, recovery after wounding at 6 h, 12 h, and 24 h. RT-PCR, western blot were applied to detect attachment-related genes’ expression and protein synthesis at 4 h and 24 h. Student’s t test was used for statistical analysis. RESULTS: Tantalum-coated titanium demonstrates a layer of homogeneously distributed nano-grains with mean diameter of 25.98 (± 14.75) nm. It was found that after tantalum deposition, human gingival fibroblasts (HGFs) adhesion, viability, proliferation, and migration were promoted in comparison to the control group. An upregulated level of Integrin β1 and FAK signaling was also detected, which might be the underlying mechanism. CONCLUSION: In the present study, adhesion, viability, proliferation, migration of human gingival fibroblasts are promoted on tantalum-coated titanium, upregulated integrin β1 and FAK might contribute to its superior performance, indicating tantalum coating can be applied in transmucosal part of dental implant. CLINICAL SIGNIFICANCE: Tantalum deposition on titanium surfaces can promote human gingival fibroblast adhesion, accordingly forming a well-organized soft tissue sealing and may contribute to a successful osseointegration. Springer Berlin Heidelberg 2021-05-03 /pmc/articles/PMC8089072/ /pubmed/33937945 http://dx.doi.org/10.1186/s40729-021-00316-z Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Zhang, Chu-nan Zhou, Lin-yi Qian, Shu-jiao Gu, Ying-xin Shi, Jun-yu Lai, Hong-chang Improved response of human gingival fibroblasts to titanium coated with micro-/nano-structured tantalum |
title | Improved response of human gingival fibroblasts to titanium coated with micro-/nano-structured tantalum |
title_full | Improved response of human gingival fibroblasts to titanium coated with micro-/nano-structured tantalum |
title_fullStr | Improved response of human gingival fibroblasts to titanium coated with micro-/nano-structured tantalum |
title_full_unstemmed | Improved response of human gingival fibroblasts to titanium coated with micro-/nano-structured tantalum |
title_short | Improved response of human gingival fibroblasts to titanium coated with micro-/nano-structured tantalum |
title_sort | improved response of human gingival fibroblasts to titanium coated with micro-/nano-structured tantalum |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8089072/ https://www.ncbi.nlm.nih.gov/pubmed/33937945 http://dx.doi.org/10.1186/s40729-021-00316-z |
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