Extracellular Vesicle Influence on Wound Healing

Diabetes is a highly prevalent disease in the world and it is involved in several chronic complications, such retinopathy, nephropathy, neuropathy, cardiovascular disease and the diabetic foot. Up to 28% of diabetic foot ulcers result in some form of amputation that lead to reduced productive activity and quality of life, as well as increased social costs. Advance in the understanding of the healing process changes in diabetic individuals is fundamental to improve diabetic foot care. Extracellular vesicles are intercellular communication agents that influence physiological and pathological processes. The objective of this research was to examine the influence of extracellular vesicles collected of diabetic individuals on cell proliferation, viability and migration in wounds. The procedures included in vitro assay of migration, senescence, cellular viability and oxide nitric production. Extracellular vesicles have been shown to stimulate the cell migration process and this function was not poorly influenced by metabolic control. References: 1. International Diabetes Federation. Diabetes Atlas, 2019. www.diabetesatlas.org. 2. Than UTT, Guanzon D, Leavesley D, Parker T. Association of Extracellular Membrane Vesicles with Cutaneous Wound Healing. Int J Mol Sci. 2017;18(5):956. 3. Van Niel G, D’Angelo G, Raposo G. Shedding light on the cell biology of extracellular vesicles. Nat Rev Mol Cell Biol. 2018;19(4):213–228. 4. Lampugnani MG. Cell migration into a wounded area in vitro. Methods Mol Biol. 1999;96:177–182.5. Sociedade Brasileira de Diabetes. Diretrizes da Sociedade de Diabetes 2017–2018. ISBN: 978-85-93746-02-4. São Paulo: Editora Clannad, 2017.6. Sociedade Brasileira de Cardiologia. Atualização da Diretrizes da Sociedade de Dislipidemia e Prevenção da Aterosclerose - 2017. ISBN: 0066-783X, vol 109, nº2, supl 1, agosto, 2017.7. Leroyer AS, Tedgui A, Boulanger CM. Microparticles and type 2 diabetes. Diabetes Metab. 2008;34 Suppl 1:S27-S32.8. International Society for Extracellular Vesicles. Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. Journal of Extracellular Vesicles, 7:1, 2018.9. Deng F, Wang S, Zhang L. Endothelial Microparticles Act as Novel Diagnostic and Therapeutic Biomarkers of Diabetes and Its Complications: A Literature Review. Biomed Res Int. 2016;2016:9802026.