Cargando…

Circulating MicroRNA-23a-5p Is a Potential Biomarker for Recovery of Bone Mass After Parathyroidectomy in Primary Hyperparathyroidism

Circulating microRNA (miRNA), a non-coding RNA molecule, is the potential biomarker for bone and mineral metabolism. Primary hyperparathyroidism (PHPT) is one of the leading causes of secondary osteoporosis. After parathyroidectomy, BMD improves as parathyroid hormone (PTH) decreases in patients wit...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Seunghyun, Hong, Namki, Kim, Kyoung Jin, Jeong, Jong Ju, Rhee, Yumie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8090189/
http://dx.doi.org/10.1210/jendso/bvab048.524
_version_ 1783687221937700864
author Lee, Seunghyun
Hong, Namki
Kim, Kyoung Jin
Jeong, Jong Ju
Rhee, Yumie
author_facet Lee, Seunghyun
Hong, Namki
Kim, Kyoung Jin
Jeong, Jong Ju
Rhee, Yumie
author_sort Lee, Seunghyun
collection PubMed
description Circulating microRNA (miRNA), a non-coding RNA molecule, is the potential biomarker for bone and mineral metabolism. Primary hyperparathyroidism (PHPT) is one of the leading causes of secondary osteoporosis. After parathyroidectomy, BMD improves as parathyroid hormone (PTH) decreases in patients with PHPT but degree of recovery in BMD vary greatly among patients. Biomarkers which are able to predict postoperative bone response may facilitate individualized approach to PHPT treatment. In this study, we aimed to investigate whether changes of miRNA related to PHPT can predict postoperative BMD recovery. A total 12 patients (10 women) who underwent parathyroidectomy for PHPT at Severance hospital from 2016 to 2019 were analyzed. A total of 15 selected miRNAs (miR-19b-3p, miR-21-5p, miR-23a-3p, miR-152-3p, miR-335-5p, miR-375, miR-532-3p, miR-24-3p, miR-100-5p, miR-125b-5p, miR-24-5p, miR-93-5p, miR-122-5p, miR-124-3p, and miR-148a-3p) from literature review were measured from pre- and pot-operative serum samples. Among pre-, post-operative level, and fold changes [the normalized gene expression(2(-ΔCt)) in postoperative period divided the normalized gene expression (2(-ΔCt)) in pre-operative period] of each miRNA, potential predictors of femoral neck BMD changes (%) as primary outcome were selected by univariable regression. The mean age of patients during parathyroidectomy was 56±16 years. PTH level and calcium level restored to normal range after parathyroidectomy in all subjects. (pre to postoperative: PTH, median 113 to 42; calcium, mean 10.6 to 9.2). Circulating level of miR-19b-3p, miR-21-5p, miR-100-5p, miR-125-5p, miR-152-3p and miR-335-5p dropped significantly after parathyroidectomy (p<0.05 for all). A fold change in miR-23a-5p showed a negative correlation with changes (%) in femur neck BMD (β coefficient=-10.58, 95% CI=-19.84 to -1.31, p=0.029). The association remained robust (β coefficient=-7.25, 95% CI=-14.17 to -0.34, p=0.042) even after adjustment for gender, age, and preoperative PTH level in multiple linear regression model. Preoperative miR-124-3p showed a positive correlation with changes in total hip BMD (β coefficient=-9.748, 95% CI= 3.740 to 15.755, p=0.005), but there was no association after adjustment for gender, age, and preoperative PTH level. In conclusion, a fold change in circulating miRNA-23a-5p predict the degree of improvement for femoral neck BMD. MiRNA have potential to serve as novel biomarker to predict treatment response in patients with PHPT, which merits further investigation.
format Online
Article
Text
id pubmed-8090189
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-80901892021-05-06 Circulating MicroRNA-23a-5p Is a Potential Biomarker for Recovery of Bone Mass After Parathyroidectomy in Primary Hyperparathyroidism Lee, Seunghyun Hong, Namki Kim, Kyoung Jin Jeong, Jong Ju Rhee, Yumie J Endocr Soc Bone and Mineral Metabolism Circulating microRNA (miRNA), a non-coding RNA molecule, is the potential biomarker for bone and mineral metabolism. Primary hyperparathyroidism (PHPT) is one of the leading causes of secondary osteoporosis. After parathyroidectomy, BMD improves as parathyroid hormone (PTH) decreases in patients with PHPT but degree of recovery in BMD vary greatly among patients. Biomarkers which are able to predict postoperative bone response may facilitate individualized approach to PHPT treatment. In this study, we aimed to investigate whether changes of miRNA related to PHPT can predict postoperative BMD recovery. A total 12 patients (10 women) who underwent parathyroidectomy for PHPT at Severance hospital from 2016 to 2019 were analyzed. A total of 15 selected miRNAs (miR-19b-3p, miR-21-5p, miR-23a-3p, miR-152-3p, miR-335-5p, miR-375, miR-532-3p, miR-24-3p, miR-100-5p, miR-125b-5p, miR-24-5p, miR-93-5p, miR-122-5p, miR-124-3p, and miR-148a-3p) from literature review were measured from pre- and pot-operative serum samples. Among pre-, post-operative level, and fold changes [the normalized gene expression(2(-ΔCt)) in postoperative period divided the normalized gene expression (2(-ΔCt)) in pre-operative period] of each miRNA, potential predictors of femoral neck BMD changes (%) as primary outcome were selected by univariable regression. The mean age of patients during parathyroidectomy was 56±16 years. PTH level and calcium level restored to normal range after parathyroidectomy in all subjects. (pre to postoperative: PTH, median 113 to 42; calcium, mean 10.6 to 9.2). Circulating level of miR-19b-3p, miR-21-5p, miR-100-5p, miR-125-5p, miR-152-3p and miR-335-5p dropped significantly after parathyroidectomy (p<0.05 for all). A fold change in miR-23a-5p showed a negative correlation with changes (%) in femur neck BMD (β coefficient=-10.58, 95% CI=-19.84 to -1.31, p=0.029). The association remained robust (β coefficient=-7.25, 95% CI=-14.17 to -0.34, p=0.042) even after adjustment for gender, age, and preoperative PTH level in multiple linear regression model. Preoperative miR-124-3p showed a positive correlation with changes in total hip BMD (β coefficient=-9.748, 95% CI= 3.740 to 15.755, p=0.005), but there was no association after adjustment for gender, age, and preoperative PTH level. In conclusion, a fold change in circulating miRNA-23a-5p predict the degree of improvement for femoral neck BMD. MiRNA have potential to serve as novel biomarker to predict treatment response in patients with PHPT, which merits further investigation. Oxford University Press 2021-05-03 /pmc/articles/PMC8090189/ http://dx.doi.org/10.1210/jendso/bvab048.524 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of the Endocrine Society. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Bone and Mineral Metabolism
Lee, Seunghyun
Hong, Namki
Kim, Kyoung Jin
Jeong, Jong Ju
Rhee, Yumie
Circulating MicroRNA-23a-5p Is a Potential Biomarker for Recovery of Bone Mass After Parathyroidectomy in Primary Hyperparathyroidism
title Circulating MicroRNA-23a-5p Is a Potential Biomarker for Recovery of Bone Mass After Parathyroidectomy in Primary Hyperparathyroidism
title_full Circulating MicroRNA-23a-5p Is a Potential Biomarker for Recovery of Bone Mass After Parathyroidectomy in Primary Hyperparathyroidism
title_fullStr Circulating MicroRNA-23a-5p Is a Potential Biomarker for Recovery of Bone Mass After Parathyroidectomy in Primary Hyperparathyroidism
title_full_unstemmed Circulating MicroRNA-23a-5p Is a Potential Biomarker for Recovery of Bone Mass After Parathyroidectomy in Primary Hyperparathyroidism
title_short Circulating MicroRNA-23a-5p Is a Potential Biomarker for Recovery of Bone Mass After Parathyroidectomy in Primary Hyperparathyroidism
title_sort circulating microrna-23a-5p is a potential biomarker for recovery of bone mass after parathyroidectomy in primary hyperparathyroidism
topic Bone and Mineral Metabolism
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8090189/
http://dx.doi.org/10.1210/jendso/bvab048.524
work_keys_str_mv AT leeseunghyun circulatingmicrorna23a5pisapotentialbiomarkerforrecoveryofbonemassafterparathyroidectomyinprimaryhyperparathyroidism
AT hongnamki circulatingmicrorna23a5pisapotentialbiomarkerforrecoveryofbonemassafterparathyroidectomyinprimaryhyperparathyroidism
AT kimkyoungjin circulatingmicrorna23a5pisapotentialbiomarkerforrecoveryofbonemassafterparathyroidectomyinprimaryhyperparathyroidism
AT jeongjongju circulatingmicrorna23a5pisapotentialbiomarkerforrecoveryofbonemassafterparathyroidectomyinprimaryhyperparathyroidism
AT rheeyumie circulatingmicrorna23a5pisapotentialbiomarkerforrecoveryofbonemassafterparathyroidectomyinprimaryhyperparathyroidism