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Circulating MicroRNA-23a-5p Is a Potential Biomarker for Recovery of Bone Mass After Parathyroidectomy in Primary Hyperparathyroidism
Circulating microRNA (miRNA), a non-coding RNA molecule, is the potential biomarker for bone and mineral metabolism. Primary hyperparathyroidism (PHPT) is one of the leading causes of secondary osteoporosis. After parathyroidectomy, BMD improves as parathyroid hormone (PTH) decreases in patients wit...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8090189/ http://dx.doi.org/10.1210/jendso/bvab048.524 |
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author | Lee, Seunghyun Hong, Namki Kim, Kyoung Jin Jeong, Jong Ju Rhee, Yumie |
author_facet | Lee, Seunghyun Hong, Namki Kim, Kyoung Jin Jeong, Jong Ju Rhee, Yumie |
author_sort | Lee, Seunghyun |
collection | PubMed |
description | Circulating microRNA (miRNA), a non-coding RNA molecule, is the potential biomarker for bone and mineral metabolism. Primary hyperparathyroidism (PHPT) is one of the leading causes of secondary osteoporosis. After parathyroidectomy, BMD improves as parathyroid hormone (PTH) decreases in patients with PHPT but degree of recovery in BMD vary greatly among patients. Biomarkers which are able to predict postoperative bone response may facilitate individualized approach to PHPT treatment. In this study, we aimed to investigate whether changes of miRNA related to PHPT can predict postoperative BMD recovery. A total 12 patients (10 women) who underwent parathyroidectomy for PHPT at Severance hospital from 2016 to 2019 were analyzed. A total of 15 selected miRNAs (miR-19b-3p, miR-21-5p, miR-23a-3p, miR-152-3p, miR-335-5p, miR-375, miR-532-3p, miR-24-3p, miR-100-5p, miR-125b-5p, miR-24-5p, miR-93-5p, miR-122-5p, miR-124-3p, and miR-148a-3p) from literature review were measured from pre- and pot-operative serum samples. Among pre-, post-operative level, and fold changes [the normalized gene expression(2(-ΔCt)) in postoperative period divided the normalized gene expression (2(-ΔCt)) in pre-operative period] of each miRNA, potential predictors of femoral neck BMD changes (%) as primary outcome were selected by univariable regression. The mean age of patients during parathyroidectomy was 56±16 years. PTH level and calcium level restored to normal range after parathyroidectomy in all subjects. (pre to postoperative: PTH, median 113 to 42; calcium, mean 10.6 to 9.2). Circulating level of miR-19b-3p, miR-21-5p, miR-100-5p, miR-125-5p, miR-152-3p and miR-335-5p dropped significantly after parathyroidectomy (p<0.05 for all). A fold change in miR-23a-5p showed a negative correlation with changes (%) in femur neck BMD (β coefficient=-10.58, 95% CI=-19.84 to -1.31, p=0.029). The association remained robust (β coefficient=-7.25, 95% CI=-14.17 to -0.34, p=0.042) even after adjustment for gender, age, and preoperative PTH level in multiple linear regression model. Preoperative miR-124-3p showed a positive correlation with changes in total hip BMD (β coefficient=-9.748, 95% CI= 3.740 to 15.755, p=0.005), but there was no association after adjustment for gender, age, and preoperative PTH level. In conclusion, a fold change in circulating miRNA-23a-5p predict the degree of improvement for femoral neck BMD. MiRNA have potential to serve as novel biomarker to predict treatment response in patients with PHPT, which merits further investigation. |
format | Online Article Text |
id | pubmed-8090189 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-80901892021-05-06 Circulating MicroRNA-23a-5p Is a Potential Biomarker for Recovery of Bone Mass After Parathyroidectomy in Primary Hyperparathyroidism Lee, Seunghyun Hong, Namki Kim, Kyoung Jin Jeong, Jong Ju Rhee, Yumie J Endocr Soc Bone and Mineral Metabolism Circulating microRNA (miRNA), a non-coding RNA molecule, is the potential biomarker for bone and mineral metabolism. Primary hyperparathyroidism (PHPT) is one of the leading causes of secondary osteoporosis. After parathyroidectomy, BMD improves as parathyroid hormone (PTH) decreases in patients with PHPT but degree of recovery in BMD vary greatly among patients. Biomarkers which are able to predict postoperative bone response may facilitate individualized approach to PHPT treatment. In this study, we aimed to investigate whether changes of miRNA related to PHPT can predict postoperative BMD recovery. A total 12 patients (10 women) who underwent parathyroidectomy for PHPT at Severance hospital from 2016 to 2019 were analyzed. A total of 15 selected miRNAs (miR-19b-3p, miR-21-5p, miR-23a-3p, miR-152-3p, miR-335-5p, miR-375, miR-532-3p, miR-24-3p, miR-100-5p, miR-125b-5p, miR-24-5p, miR-93-5p, miR-122-5p, miR-124-3p, and miR-148a-3p) from literature review were measured from pre- and pot-operative serum samples. Among pre-, post-operative level, and fold changes [the normalized gene expression(2(-ΔCt)) in postoperative period divided the normalized gene expression (2(-ΔCt)) in pre-operative period] of each miRNA, potential predictors of femoral neck BMD changes (%) as primary outcome were selected by univariable regression. The mean age of patients during parathyroidectomy was 56±16 years. PTH level and calcium level restored to normal range after parathyroidectomy in all subjects. (pre to postoperative: PTH, median 113 to 42; calcium, mean 10.6 to 9.2). Circulating level of miR-19b-3p, miR-21-5p, miR-100-5p, miR-125-5p, miR-152-3p and miR-335-5p dropped significantly after parathyroidectomy (p<0.05 for all). A fold change in miR-23a-5p showed a negative correlation with changes (%) in femur neck BMD (β coefficient=-10.58, 95% CI=-19.84 to -1.31, p=0.029). The association remained robust (β coefficient=-7.25, 95% CI=-14.17 to -0.34, p=0.042) even after adjustment for gender, age, and preoperative PTH level in multiple linear regression model. Preoperative miR-124-3p showed a positive correlation with changes in total hip BMD (β coefficient=-9.748, 95% CI= 3.740 to 15.755, p=0.005), but there was no association after adjustment for gender, age, and preoperative PTH level. In conclusion, a fold change in circulating miRNA-23a-5p predict the degree of improvement for femoral neck BMD. MiRNA have potential to serve as novel biomarker to predict treatment response in patients with PHPT, which merits further investigation. Oxford University Press 2021-05-03 /pmc/articles/PMC8090189/ http://dx.doi.org/10.1210/jendso/bvab048.524 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of the Endocrine Society. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Bone and Mineral Metabolism Lee, Seunghyun Hong, Namki Kim, Kyoung Jin Jeong, Jong Ju Rhee, Yumie Circulating MicroRNA-23a-5p Is a Potential Biomarker for Recovery of Bone Mass After Parathyroidectomy in Primary Hyperparathyroidism |
title | Circulating MicroRNA-23a-5p Is a Potential Biomarker for Recovery of Bone Mass After Parathyroidectomy in Primary Hyperparathyroidism |
title_full | Circulating MicroRNA-23a-5p Is a Potential Biomarker for Recovery of Bone Mass After Parathyroidectomy in Primary Hyperparathyroidism |
title_fullStr | Circulating MicroRNA-23a-5p Is a Potential Biomarker for Recovery of Bone Mass After Parathyroidectomy in Primary Hyperparathyroidism |
title_full_unstemmed | Circulating MicroRNA-23a-5p Is a Potential Biomarker for Recovery of Bone Mass After Parathyroidectomy in Primary Hyperparathyroidism |
title_short | Circulating MicroRNA-23a-5p Is a Potential Biomarker for Recovery of Bone Mass After Parathyroidectomy in Primary Hyperparathyroidism |
title_sort | circulating microrna-23a-5p is a potential biomarker for recovery of bone mass after parathyroidectomy in primary hyperparathyroidism |
topic | Bone and Mineral Metabolism |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8090189/ http://dx.doi.org/10.1210/jendso/bvab048.524 |
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