Cargando…

Manipulation of genes could inhibit SARS-CoV-2 infection that causes COVID-19 pandemics

The year 2020 witnessed an unpredictable pandemic situation due to novel coronavirus (COVID-19) outbreaks. This condition can be more severe if the patient has comorbidities. Failure of viable treatment for such viral infection caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) i...

Descripción completa

Detalles Bibliográficos
Autores principales: Banerjee, Arnab, Mukherjee, Sandip, Maji, Bithin K
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8090827/
https://www.ncbi.nlm.nih.gov/pubmed/33899542
http://dx.doi.org/10.1177/15353702211008106
_version_ 1783687373621559296
author Banerjee, Arnab
Mukherjee, Sandip
Maji, Bithin K
author_facet Banerjee, Arnab
Mukherjee, Sandip
Maji, Bithin K
author_sort Banerjee, Arnab
collection PubMed
description The year 2020 witnessed an unpredictable pandemic situation due to novel coronavirus (COVID-19) outbreaks. This condition can be more severe if the patient has comorbidities. Failure of viable treatment for such viral infection caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is due to lack of identification. Thus, modern and productive biotechnology-based tools are being used to manipulate target genes by introducing the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas (CRISPR-associated) system. Moreover, it has now been used as a tool to inhibit viral replication. Hence, it can be hypothesized that the CRISPR/Cas system can be a viable tool to target both the SARS-CoV-2 genome with specific target RNA sequence and host factors to destroy the SARS-CoV-2 community via inhibition of viral replication and infection. Moreover, comorbidities and COVID-19 escalate the rate of mortality globally, and as a result, we have faced this pandemic. CRISPR/Cas-mediated genetic manipulation to knockdown viral sequences may be a preventive strategy against such pandemic caused by SARS-CoV-2. Furthermore, prophylactic antiviral CRISPR in human cells (PAC-MAN) along with CRISPR/Cas13d efficiently degrades the specific RNA sequence to inhibit viral replication. Therefore, we suggest that CRISPR/Cas system with PAC-MAN could be a useful tool to fight against such a global pandemic caused by SARS-CoV-2. This is an alternative preventive approach of management against the pandemic to destroy the target sequence of RNA in SARS-CoV-2 by viral inhibition.
format Online
Article
Text
id pubmed-8090827
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher SAGE Publications
record_format MEDLINE/PubMed
spelling pubmed-80908272021-05-03 Manipulation of genes could inhibit SARS-CoV-2 infection that causes COVID-19 pandemics Banerjee, Arnab Mukherjee, Sandip Maji, Bithin K Exp Biol Med (Maywood) Minireview The year 2020 witnessed an unpredictable pandemic situation due to novel coronavirus (COVID-19) outbreaks. This condition can be more severe if the patient has comorbidities. Failure of viable treatment for such viral infection caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is due to lack of identification. Thus, modern and productive biotechnology-based tools are being used to manipulate target genes by introducing the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas (CRISPR-associated) system. Moreover, it has now been used as a tool to inhibit viral replication. Hence, it can be hypothesized that the CRISPR/Cas system can be a viable tool to target both the SARS-CoV-2 genome with specific target RNA sequence and host factors to destroy the SARS-CoV-2 community via inhibition of viral replication and infection. Moreover, comorbidities and COVID-19 escalate the rate of mortality globally, and as a result, we have faced this pandemic. CRISPR/Cas-mediated genetic manipulation to knockdown viral sequences may be a preventive strategy against such pandemic caused by SARS-CoV-2. Furthermore, prophylactic antiviral CRISPR in human cells (PAC-MAN) along with CRISPR/Cas13d efficiently degrades the specific RNA sequence to inhibit viral replication. Therefore, we suggest that CRISPR/Cas system with PAC-MAN could be a useful tool to fight against such a global pandemic caused by SARS-CoV-2. This is an alternative preventive approach of management against the pandemic to destroy the target sequence of RNA in SARS-CoV-2 by viral inhibition. SAGE Publications 2021-04-25 2021-07 /pmc/articles/PMC8090827/ /pubmed/33899542 http://dx.doi.org/10.1177/15353702211008106 Text en © 2021 by the Society for Experimental Biology and Medicine https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Minireview
Banerjee, Arnab
Mukherjee, Sandip
Maji, Bithin K
Manipulation of genes could inhibit SARS-CoV-2 infection that causes COVID-19 pandemics
title Manipulation of genes could inhibit SARS-CoV-2 infection that causes COVID-19 pandemics
title_full Manipulation of genes could inhibit SARS-CoV-2 infection that causes COVID-19 pandemics
title_fullStr Manipulation of genes could inhibit SARS-CoV-2 infection that causes COVID-19 pandemics
title_full_unstemmed Manipulation of genes could inhibit SARS-CoV-2 infection that causes COVID-19 pandemics
title_short Manipulation of genes could inhibit SARS-CoV-2 infection that causes COVID-19 pandemics
title_sort manipulation of genes could inhibit sars-cov-2 infection that causes covid-19 pandemics
topic Minireview
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8090827/
https://www.ncbi.nlm.nih.gov/pubmed/33899542
http://dx.doi.org/10.1177/15353702211008106
work_keys_str_mv AT banerjeearnab manipulationofgenescouldinhibitsarscov2infectionthatcausescovid19pandemics
AT mukherjeesandip manipulationofgenescouldinhibitsarscov2infectionthatcausescovid19pandemics
AT majibithink manipulationofgenescouldinhibitsarscov2infectionthatcausescovid19pandemics