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In utero exposure to chlordecone affects histone modifications and activates LINE-1 in cord blood

Environmental factors can induce detrimental consequences into adulthood life. In this study, we examined the epigenetic effects induced by in utero chlordecone (CD) exposure on human male cord blood as well as in blood-derived Ke-37 cell line. Genome-wide analysis of histone H3K4me3 distribution re...

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Autores principales: Legoff, Louis, D’Cruz, Shereen Cynthia, Bouchekhchoukha, Katia, Monfort, Christine, Jaulin, Christian, Multigner, Luc, Smagulova, Fatima
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Life Science Alliance LLC 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8091598/
https://www.ncbi.nlm.nih.gov/pubmed/33837044
http://dx.doi.org/10.26508/lsa.202000944
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author Legoff, Louis
D’Cruz, Shereen Cynthia
Bouchekhchoukha, Katia
Monfort, Christine
Jaulin, Christian
Multigner, Luc
Smagulova, Fatima
author_facet Legoff, Louis
D’Cruz, Shereen Cynthia
Bouchekhchoukha, Katia
Monfort, Christine
Jaulin, Christian
Multigner, Luc
Smagulova, Fatima
author_sort Legoff, Louis
collection PubMed
description Environmental factors can induce detrimental consequences into adulthood life. In this study, we examined the epigenetic effects induced by in utero chlordecone (CD) exposure on human male cord blood as well as in blood-derived Ke-37 cell line. Genome-wide analysis of histone H3K4me3 distribution revealed that genes related to chromosome segregation, chromatin organization, and cell cycle have altered occupancy in their promoters. The affected regions were enriched in ESR1, SP family, and IKZF1 binding motifs. We also observed a global reduction in H3K9me3, markedly in repeated sequences of the genome. Decrease in H3K9me3 after CD exposure correlates with decreased methylation in LINE-1 promoters and telomere length extension. These observations on human cord blood were assessed in the Ke-37 human cell line. H3K4me3 and the expression of genes related to immune response, DNA repair, and chromatin organization, which were affected in human cord blood were also altered in CD-exposed Ke-37 cells. Our data suggest that developmental exposure to CD leads to profound changes in histone modification patterns and affects the processes controlled by them in human cord blood.
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spelling pubmed-80915982021-05-12 In utero exposure to chlordecone affects histone modifications and activates LINE-1 in cord blood Legoff, Louis D’Cruz, Shereen Cynthia Bouchekhchoukha, Katia Monfort, Christine Jaulin, Christian Multigner, Luc Smagulova, Fatima Life Sci Alliance Research Articles Environmental factors can induce detrimental consequences into adulthood life. In this study, we examined the epigenetic effects induced by in utero chlordecone (CD) exposure on human male cord blood as well as in blood-derived Ke-37 cell line. Genome-wide analysis of histone H3K4me3 distribution revealed that genes related to chromosome segregation, chromatin organization, and cell cycle have altered occupancy in their promoters. The affected regions were enriched in ESR1, SP family, and IKZF1 binding motifs. We also observed a global reduction in H3K9me3, markedly in repeated sequences of the genome. Decrease in H3K9me3 after CD exposure correlates with decreased methylation in LINE-1 promoters and telomere length extension. These observations on human cord blood were assessed in the Ke-37 human cell line. H3K4me3 and the expression of genes related to immune response, DNA repair, and chromatin organization, which were affected in human cord blood were also altered in CD-exposed Ke-37 cells. Our data suggest that developmental exposure to CD leads to profound changes in histone modification patterns and affects the processes controlled by them in human cord blood. Life Science Alliance LLC 2021-04-09 /pmc/articles/PMC8091598/ /pubmed/33837044 http://dx.doi.org/10.26508/lsa.202000944 Text en © 2021 Legoff et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Articles
Legoff, Louis
D’Cruz, Shereen Cynthia
Bouchekhchoukha, Katia
Monfort, Christine
Jaulin, Christian
Multigner, Luc
Smagulova, Fatima
In utero exposure to chlordecone affects histone modifications and activates LINE-1 in cord blood
title In utero exposure to chlordecone affects histone modifications and activates LINE-1 in cord blood
title_full In utero exposure to chlordecone affects histone modifications and activates LINE-1 in cord blood
title_fullStr In utero exposure to chlordecone affects histone modifications and activates LINE-1 in cord blood
title_full_unstemmed In utero exposure to chlordecone affects histone modifications and activates LINE-1 in cord blood
title_short In utero exposure to chlordecone affects histone modifications and activates LINE-1 in cord blood
title_sort in utero exposure to chlordecone affects histone modifications and activates line-1 in cord blood
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8091598/
https://www.ncbi.nlm.nih.gov/pubmed/33837044
http://dx.doi.org/10.26508/lsa.202000944
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