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A novel nanoemulsion-based microalgal growth medium for enhanced biomass production

BACKGROUND: Microalgae are well-established feedstocks for applications ranging from biofuels to valuable pigments and therapeutic proteins. However, the low biomass productivity using commercially available growth mediums is a roadblock for its mass production. This work describes a strategy to boo...

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Autores principales: Nigam, Harshita, Malik, Anushree, Singh, Vikram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8091788/
https://www.ncbi.nlm.nih.gov/pubmed/33941238
http://dx.doi.org/10.1186/s13068-021-01960-8
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author Nigam, Harshita
Malik, Anushree
Singh, Vikram
author_facet Nigam, Harshita
Malik, Anushree
Singh, Vikram
author_sort Nigam, Harshita
collection PubMed
description BACKGROUND: Microalgae are well-established feedstocks for applications ranging from biofuels to valuable pigments and therapeutic proteins. However, the low biomass productivity using commercially available growth mediums is a roadblock for its mass production. This work describes a strategy to boost algal biomass productivity by using an effective CO(2) supplement. RESULTS: In the present study, a novel nanoemulsion-based media has been tested for the growth of freshwater microalgae strain Chlorella pyrenoidosa. Two different nanoemulsion-based media were developed using 1% silicone oil nanoemulsion (1% SE) and 1% paraffin oil nanoemulsion (1% PE) supplemented in Blue-green 11 media (BG11). After 12 days of cultivation, biomass yield was found highest in 1% PE followed by 1% SE and control, i.e., 3.20, 2.75, and 1.03 g L(−1), respectively. The chlorophyll-a synthesis was improved by 76% in 1% SE and 53% in 1% PE compared with control. The respective microalgal cell numbers for 1% PE, 1% SE and control measured using the cell counter were 3.00 × 10(6), 2.40 × 10(6), and 1.34 × 10(6) cells mL(−1). The effective CO(2) absorption tendency of the emulsion was highlighted as the key mechanism for enhanced algal growth and biomass production. On the biochemical characterization of the produced biomass, it was found that the nanoemulsion-cultivated C. pyrenoidosa had increased lipid (1% PE = 26.80%, 1% SE = 23.60%) and carbohydrates (1% PE = 17.20%, 1% SE = 18.90%) content compared to the control (lipid = 18.05%, carbohydrates = 13.60%). CONCLUSIONS: This study describes a novel nanoemulsion which potentially acts as an effective CO(2) supplement for microalgal growth media thereby increasing the growth of microalgal cells. Further, nanoemulsion-cultivated microalgal biomass depicts an increase in lipid and carbohydrate content. The approach provides high microalgal biomass productivity without altering morphological characteristics like cell shape and size as revealed by field emission scanning electron microscope (FESEM) images. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13068-021-01960-8.
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spelling pubmed-80917882021-05-04 A novel nanoemulsion-based microalgal growth medium for enhanced biomass production Nigam, Harshita Malik, Anushree Singh, Vikram Biotechnol Biofuels Research BACKGROUND: Microalgae are well-established feedstocks for applications ranging from biofuels to valuable pigments and therapeutic proteins. However, the low biomass productivity using commercially available growth mediums is a roadblock for its mass production. This work describes a strategy to boost algal biomass productivity by using an effective CO(2) supplement. RESULTS: In the present study, a novel nanoemulsion-based media has been tested for the growth of freshwater microalgae strain Chlorella pyrenoidosa. Two different nanoemulsion-based media were developed using 1% silicone oil nanoemulsion (1% SE) and 1% paraffin oil nanoemulsion (1% PE) supplemented in Blue-green 11 media (BG11). After 12 days of cultivation, biomass yield was found highest in 1% PE followed by 1% SE and control, i.e., 3.20, 2.75, and 1.03 g L(−1), respectively. The chlorophyll-a synthesis was improved by 76% in 1% SE and 53% in 1% PE compared with control. The respective microalgal cell numbers for 1% PE, 1% SE and control measured using the cell counter were 3.00 × 10(6), 2.40 × 10(6), and 1.34 × 10(6) cells mL(−1). The effective CO(2) absorption tendency of the emulsion was highlighted as the key mechanism for enhanced algal growth and biomass production. On the biochemical characterization of the produced biomass, it was found that the nanoemulsion-cultivated C. pyrenoidosa had increased lipid (1% PE = 26.80%, 1% SE = 23.60%) and carbohydrates (1% PE = 17.20%, 1% SE = 18.90%) content compared to the control (lipid = 18.05%, carbohydrates = 13.60%). CONCLUSIONS: This study describes a novel nanoemulsion which potentially acts as an effective CO(2) supplement for microalgal growth media thereby increasing the growth of microalgal cells. Further, nanoemulsion-cultivated microalgal biomass depicts an increase in lipid and carbohydrate content. The approach provides high microalgal biomass productivity without altering morphological characteristics like cell shape and size as revealed by field emission scanning electron microscope (FESEM) images. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13068-021-01960-8. BioMed Central 2021-04-30 /pmc/articles/PMC8091788/ /pubmed/33941238 http://dx.doi.org/10.1186/s13068-021-01960-8 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Nigam, Harshita
Malik, Anushree
Singh, Vikram
A novel nanoemulsion-based microalgal growth medium for enhanced biomass production
title A novel nanoemulsion-based microalgal growth medium for enhanced biomass production
title_full A novel nanoemulsion-based microalgal growth medium for enhanced biomass production
title_fullStr A novel nanoemulsion-based microalgal growth medium for enhanced biomass production
title_full_unstemmed A novel nanoemulsion-based microalgal growth medium for enhanced biomass production
title_short A novel nanoemulsion-based microalgal growth medium for enhanced biomass production
title_sort novel nanoemulsion-based microalgal growth medium for enhanced biomass production
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8091788/
https://www.ncbi.nlm.nih.gov/pubmed/33941238
http://dx.doi.org/10.1186/s13068-021-01960-8
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