Reflex Detection of Ciprofloxacin Resistance in Neisseria gonorrhoeae by Use of the SpeeDx ResistancePlus GC Assay

Resistance-guided therapy (RGT) for gonorrhea may reduce unnecessary use of broad-spectrum antibiotics. When reflexed from the Aptima Combo 2 assay, the ResistancePlus GC assay demonstrated 94.8% sensitivity and 100.0% specificity for Neisseria gonorrhoeae detection. Of the 379 concordant N. gonorrh...

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Detalles Bibliográficos
Autores principales: Lee, Darren Y. J., Ashcroft, Melinda M., Chow, Eric P. F., Sait, Michelle, De Petra, Vesna, Tschaepe, Marlene, Lange, Sigrid, Taiaroa, George, Bradshaw, Catriona S., Whiley, David M., Fairley, Christopher K., Howden, Benjamin P., Chen, Marcus Y., Pasricha, Shivani, Williamson, Deborah A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Bacteriology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8091848/
https://www.ncbi.nlm.nih.gov/pubmed/33658263
http://dx.doi.org/10.1128/JCM.00089-21
Descripción
Sumario:Resistance-guided therapy (RGT) for gonorrhea may reduce unnecessary use of broad-spectrum antibiotics. When reflexed from the Aptima Combo 2 assay, the ResistancePlus GC assay demonstrated 94.8% sensitivity and 100.0% specificity for Neisseria gonorrhoeae detection. Of the 379 concordant N. gonorrhoeae-positive samples, 86.8% were found to possess the gyrA S91F mutation, which was highly predictive for ciprofloxacin resistance and stable across 3,144 publicly available N. gonorrhoeae genomes. Our work supports the feasibility of implementing RGT for gonorrhea into routine molecular workflows.

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